公开(公告)号：US20240254460A1

公开(公告)日：2024-08-01

申请号：US17254376

申请日：2020-11-13

Applicant: Daan Gene Co., Ltd.

Inventor： Xiwen JIANG ,  Aishan LIU ,  Zhouquan WANG ,  Sangsang ZHENG ,  Jiguang ZHAO ,  Xianlan LIAN ,  Xiaocheng XIE ,  Xuelan LU ,  Wei ZHANG ,  Haojian XU ,  Zhikeng FANG ,  Zhijian WU

IPC: C12N9/12 ,  C12N15/10

CPC classification number: C12N9/1276 ,  C12N15/1096 ,  C12Y207/07049

Abstract: The present invention provides a high temperature resistant reverse transcriptase mutant and its use. In particular, the present invention constructs a reverse transcriptase (M-MLV) mutant library, and the mutants with improved thermostability and higher amplification efficiency are finally screened out by stepwise screening. Under high temperature conditions, the reverse transcription efficiency of the reverse transcriptase mutant of the present invention is greatly improved compared to the wild type.

公开(公告)号：US20250019751A1

公开(公告)日：2025-01-16

申请号：US18713016

申请日：2022-07-04

Applicant: DAAN GENE CO., LTD.

Inventor： Xiwen JIANG ,  Aishan LIU ,  Zhouquan WANG ,  Wenya ZHU

IPC: C12Q1/6844

Abstract: Provided are a reverse-transcription amplification system and method based on recombinase polymerase amplification (RPA) technology. specifically, provided are that an RNA template in a sample can be effectively amplified after a reverse transcriptase and RNasin are added to an RPA system, and on this basis, the nucleic acid amplification efficiency of a reverse-transcription real-time fluorescent RPA system (RT-qRPA) can be effectively improved by means of adding inorganic pyrophosphatase (IPP).