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    TAQ POLYMERASE MUTANT, AND PREPARATION METHOD THEREFOR AND USE THEREOF
    1.
    发明申请
    TAQ POLYMERASE MUTANT, AND PREPARATION METHOD THEREFOR AND USE THEREOF 有权

    公开(公告)号：US20240392266A1

    公开(公告)日：2024-11-28

    申请号：US18694726

    申请日：2022-06-22

    Applicant: DAAN GENE CO., LTD.

    Inventor： Xiwen JIANG ,  Aishan LIU ,  Wei ZHANG ,  Xiaocheng XIE ,  Xuelan LU

    IPC: C12N9/12 ,  C12N15/70

    Abstract: Provided are a Taq polymerase mutant, a preparation method therefor and the use thereof. The Taq polymerase mutant includes an amino acid sequence having at least 70% identity to the amino acid sequence shown in SEQ ID NO:2, wherein the amino acid sequence has mutations occurring at one or more positions selected from the group consisting of: P40, L125, G200, D335, G499, E634, F769 or a combination thereof. The provided Taq polymerase mutant has high amplification activity, can obtain more amplification products compared with wild-type Taq polymerase under the same number of PCR cycles, and moreover, is resistant to whole blood and high salt and has low 5′-3′ exonuclease activity.

    METHOD FOR PREPARING CONSTANT-TEMPERATURE AMPLIFICATION MIXED ENZYME SYSTEM
    3.
    发明申请
    METHOD FOR PREPARING CONSTANT-TEMPERATURE AMPLIFICATION MIXED ENZYME SYSTEM 有权

    公开(公告)号：US20250002986A1

    公开(公告)日：2025-01-02

    申请号：US18713019

    申请日：2022-07-04

    Applicant: DAAN GENE CO., LTD.

    Inventor： Xiwen JIANG ,  Xianlan LIAN ,  Xiaocheng XIE ,  Xuelan LU ,  Haojian XU

    IPC: C12Q1/6851

    Abstract: Provided in the present invention is a method for preparing a constant-temperature amplification mixed enzyme system. Specifically, disclosed in the present invention is a method comprising: removing the vast majority of glycerol from a prepared glycerol-containing RPA enzyme system by means of dialysis, and then concentrating the enzyme system containing trace glycerol by using an ultrafiltration method. Experiments show that the amplification performance of an RPA mixed enzyme system prepared by means of such method is significantly improved.

    KIT FOR TESTING COVID-19 ANTIGEN AND METHOD FOR TESTING SAME
    4.
    发明公开
    KIT FOR TESTING COVID-19 ANTIGEN AND METHOD FOR TESTING SAME 审中-公开

    公开(公告)号：US20230152315A1

    公开(公告)日：2023-05-18

    申请号：US17721382

    申请日：2022-04-15

    Applicant: DAAN GENE CO., LTD.

    Inventor： Xiwen Jiang ,  Wenchuang Qi ,  Shuang Liu ,  Xiuhua Pan ,  Songkang Su ,  Hong Xu

    IPC: G01N33/569 ,  G01N33/577 ,  G01N33/558 ,  G01N33/543

    CPC classification number: G01N33/56983 ,  G01N33/577 ,  G01N33/558 ,  G01N33/54388 ,  G01N2333/165

    Abstract: Disclosed is a kit for testing a COVID-19 antigen. The kit includes a test card, a conjugate pad and a reaction pad being arranged on the test card; wherein the conjugate pad is provided with a labeled antibody Ab1 and a labeled antibody Ab3 of the COVID-19 antigen, and a quality control line and a test line are arranged in parallel with an interval on the reaction pad, the test line being provided with a coating antibody Ab2, and the quality control line being provided with a coating antibody Ab4. With the kit, by the AIE immunofluorescence chromatographic technique, the AIE fluorescent microspheres are used as labels, and the test result of the test card is directly determined and read by using a fluorescent flashlight, or the test result is read by using a fluorescent tester. Such kits have the advantages of high sensitivity, strong specificity and simple operation.

    Nucleic Acid Detection Kit For Novel Coronavirus 2019-nCoV
    5.
    发明申请
    Nucleic Acid Detection Kit For Novel Coronavirus 2019-nCoV 有权

    公开(公告)号：US20230070496A1

    公开(公告)日：2023-03-09

    申请号：US17760195

    申请日：2020-12-30

    Applicant: Daan Gene Co., Ltd.

    Inventor： Xiwen JIANG ,  Jian FAN ,  Hailong PENG

    IPC: C12Q1/70 ,  C12Q1/686 ,  C12Q1/6876

    Abstract: Provided is a nucleic acid detection kit for novel coronavirus 2019-nCoV, and in particular, provided are a kit and a method for multiple detection of the nucleic acid of the novel coronavirus 2019-nCoV. Three nucleic acid targets of the novel coronavirus 2019-nCoV can be detected at the same time. False positives can be prevented by means of mutual authentication among different targets, and detection omissions, which may be caused by mutations, are confirmed, such that the accuracy of virus identification is significantly improved.

    HAND-HELD NUCLEIC ACID DETECTION APPARATUS EQUIPPED WITH MICROFLUIDIC CHIP, AND USE METHOD THEREOF
    8.
    发明公开
    HAND-HELD NUCLEIC ACID DETECTION APPARATUS EQUIPPED WITH MICROFLUIDIC CHIP, AND USE METHOD THEREOF 审中-公开

    公开(公告)号：US20230383222A1

    公开(公告)日：2023-11-30

    申请号：US18061481

    申请日：2022-12-05

    Applicant: DAAN GENE CO., LTD.

    Inventor： XIWEN JIANG ,  LINBO LIU ,  HAITAO SHU ,  JUNJIE LI

    IPC: C12M1/24 ,  C12Q1/68 ,  B01L3/00

    CPC classification number: C12M1/24 ,  C12Q1/68 ,  B01L3/50273 ,  B01L2200/10 ,  B01L2200/0689

    Abstract: Disclosed are a hand-held nucleic acid detection apparatus equipped with microfluidic chip, and a use method thereof. The detection apparatus includes a sample collection tube, a chip connector, and a detection chip. The chip connector is provided with a piercing tube configured to pierce the sample collection tube. The detection chip is connected to the chip connector. The detection chip is provided with a plurality of chambers and a plurality of flow channels. The flow channels are in communication with the piercing tube and the chambers. The reaction reagent and all the reaction processes are integrated on a chip, with no need of solution injection by virtue of an external instrument, solution transfer or the like operations, and the nucleic acid detection may be carried out even in the case of no dedicated experiment sites or special conditions.

    PRESERVATIVE FOR IN VITRO DIAGNOSTIC REAGENT AND USE THEREOF
    10.
    发明申请
    PRESERVATIVE FOR IN VITRO DIAGNOSTIC REAGENT AND USE THEREOF 有权

    公开(公告)号：US20230115749A1

    公开(公告)日：2023-04-13

    申请号：US17726486

    申请日：2022-04-21

    Applicant: DAAN GENE CO., LTD.

    Inventor： Xiwen Jiang ,  Wenchuang Qi ,  Runfeng Wu ,  Shaoqing Duan ,  Junxing Jian ,  Xue Diao

    IPC: C12Q1/52 ,  G01N33/92

    Abstract: The embodiments of the present application, belonging to the technical field of medical test and assay, and provide a preservative for an in vitro diagnostic reagent. The preservative includes a combination of a sulfadoxine solution and a dimethoprim solution, wherein a molar ratio of sulfadoxine in the sulfadoxine solution to dimethoprim in the dimethoprim solution is from 0.002 to 1. The present disclosure also provides use of the preservative for the in vitro diagnostic reagent. The technical solutions according to the embodiments of the present disclosure improve stability of the reagent, do not affect reactions of the reagent, and may be extensively applied.

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