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    NUCLEIC ACID AMPLIFICATION PROCESSES
    5.
    发明公开
    NUCLEIC ACID AMPLIFICATION PROCESSES 审中-公开

    公开(公告)号：US20230340579A1

    公开(公告)日：2023-10-26

    申请号：US18102031

    申请日：2023-01-26

    申请人： LumiraDx UK Ltd.

    发明人： Jarrod Provins ,  Daiwei Shen ,  Bryan Kraynack

    IPC分类号： C12Q1/6844 ,  B01L7/00 ,  C12Q1/6848 ,  C12Q1/6853

    CPC分类号： C12Q1/6844 ,  B01L7/52 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q2521/307 ,  C12Q2525/131 ,  C12Q2527/101 ,  C12Q2537/137 ,  C12Q2521/531 ,  C12Q2521/107 ,  C12Q2525/113 ,  C12Q2525/301 ,  C12Q2521/101

    摘要： Disclosed is a method of performing a non-isothermal nucleic acid amplification reaction, the method comprising the steps of: (a) mixing a target sequence with one or more complementary single stranded primers in conditions which permit a hybridisation event in which the primers hybridise to the target, which hybridisation event, directly or indirectly, leads to the formation of a duplex structure comprising two nicking sites disposed at or near opposite ends of the duplex; and performing an amplification process by; (b) causing a nick at each of said nicking sites in the strands of the duplex; (c) using a polymerase to extend the nicked strands so as to form newly synthesised nucleic acid, which extension with the polymerase recreates nicking sites; (d) repeating steps (b) and (c) as desired so as to cause the production of multiple copies of the newly synthesised nucleic acid; characterised in that the temperature at which the method is performed is non-isothermal, and subject to a reduction of at least 2° C. during the amplification process of steps (b)-(d).

    AMPLIFICATION AND DETECTION OF RIBONUCLEIC ACIDS
    10.
    发明申请
    AMPLIFICATION AND DETECTION OF RIBONUCLEIC ACIDS 审中-公开

    公开(公告)号：US20190211387A1

    公开(公告)日：2019-07-11

    申请号：US16363508

    申请日：2019-03-25

    申请人： APPLIED BIOSYSTEM, LLC

    发明人： R. Scott KUERSTEN

    IPC分类号： C12Q1/6855 ,  C12Q1/68 ,  C12Q1/6806 ,  C12N15/10

    CPC分类号： C12Q1/6855 ,  C12N15/1096 ,  C12Q1/68 ,  C12Q1/6806 ,  C12Q2565/125 ,  C12Q2525/207 ,  C12Q2521/107 ,  C12Q2565/137

    摘要： Compositions, methods, and kits for detecting one or more species of RNA molecules are disclosed. In one embodiment, a first adaptor and a second adaptor are ligated to the RNA molecule using a polypeptide comprising double-strand specific RNA ligase activity, without an intervening purification step. The ligated product is reverse transcribed, then at least some of the ribonucleosides in the reverse transcription product are removed. Primers are added and amplified products are generated. In certain embodiments, the sequence of at least part of at least one species of amplified product is determined and at least part of the corresponding RNA molecule is determined. In some embodiments, at least some of the amplified product species are detected, directly or indirectly, allowing the presence and/or quantity of the RNA molecule of interest to be determined.