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    NUCLEIC ACID AMPLIFICATION PROCESSES
    2.
    发明公开
    NUCLEIC ACID AMPLIFICATION PROCESSES 审中-公开

    公开(公告)号：US20230340579A1

    公开(公告)日：2023-10-26

    申请号：US18102031

    申请日：2023-01-26

    Applicant: LumiraDx UK Ltd.

    Inventor： Jarrod Provins ,  Daiwei Shen ,  Bryan Kraynack

    IPC: C12Q1/6844 ,  B01L7/00 ,  C12Q1/6848 ,  C12Q1/6853

    CPC classification number: C12Q1/6844 ,  B01L7/52 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q2521/307 ,  C12Q2525/131 ,  C12Q2527/101 ,  C12Q2537/137 ,  C12Q2521/531 ,  C12Q2521/107 ,  C12Q2525/113 ,  C12Q2525/301 ,  C12Q2521/101

    Abstract: Disclosed is a method of performing a non-isothermal nucleic acid amplification reaction, the method comprising the steps of: (a) mixing a target sequence with one or more complementary single stranded primers in conditions which permit a hybridisation event in which the primers hybridise to the target, which hybridisation event, directly or indirectly, leads to the formation of a duplex structure comprising two nicking sites disposed at or near opposite ends of the duplex; and performing an amplification process by; (b) causing a nick at each of said nicking sites in the strands of the duplex; (c) using a polymerase to extend the nicked strands so as to form newly synthesised nucleic acid, which extension with the polymerase recreates nicking sites; (d) repeating steps (b) and (c) as desired so as to cause the production of multiple copies of the newly synthesised nucleic acid; characterised in that the temperature at which the method is performed is non-isothermal, and subject to a reduction of at least 2° C. during the amplification process of steps (b)-(d).

    In or relating to uncleic acid amplification processes
    3.
    发明授权
    In or relating to uncleic acid amplification processes 有权

    公开(公告)号：US11591643B2

    公开(公告)日：2023-02-28

    申请号：US16313750

    申请日：2017-06-30

    Applicant: LumiraDx UK Ltd.

    Inventor： Jarrod Provins ,  Daiwei Shen ,  Bryan Kraynack

    IPC: C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6853 ,  B01L7/00

    Abstract: Disclosed is a method of performing a non-isothermal nucleic acid amplification reaction, wherein the temperature at which the method is performed is non-isothermal and subject to a reduction of at least 2° C. during amplification process steps. The present invention provides an improved nucleic acid amplification technique having one or more advantages over existing techniques including, for example, decreased reaction time, increased yield, and decreased non-specific amplification products.

    AMPLIFICATION OF NUCLEIC ACIDS
    5.
    发明公开
    AMPLIFICATION OF NUCLEIC ACIDS 审中-公开

    公开(公告)号：US20240026433A1

    公开(公告)日：2024-01-25

    申请号：US18304282

    申请日：2023-04-20

    Applicant: LumiraDx UK Ltd.

    Inventor： Daiwei Shen ,  Bryan Kraynack ,  Victor Perez ,  Jarrod Provins

    IPC: C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/6853

    CPC classification number: C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q2521/101 ,  C12Q2521/107 ,  C12Q2521/307 ,  C12Q2525/113 ,  C12Q2525/131 ,  C12Q2525/301 ,  C12Q2527/101 ,  C12Q2537/137

    Abstract: Disclosed is a method of performing a non-isothermal nucleic acid amplification reaction, the method comprising the steps of: (a) mixing a target sequence with one or more complementary single stranded primers in conditions which permit a hybridisation event in which the primers hybridise to the target, which hybridisation event, directly or indirectly, leads to the formation of a duplex structure comprising two nicking sites disposed at or near opposite ends of the duplex; and performing an amplification process by; (b) using a nicking enzyme to cause a nick at each of said nicking sites in the strands of the duplex; (c) using a polymerase to extend the nicked strands to as to form newly synthesised nucleic acid, which extension with the polymerase recreates nicking sites; (d) repeating steps (b) and (c) as desired so as to cause the production of multiple copies of the newly synthesised nucleic acid; characterised in that the temperature at which the method is performed is non-isothermal, and subject to shuttling, a plurality of times, between an upper temperature and a lower temperature during the amplification process of steps (b)-(d), wherein at the upper temperature, one of said polymerase or nicking enzyme is more active than the other of said enzymes, such that there is a disparity in the activity of the enzymes, and at the lower temperature the disparity in the activity of the enzymes is reduced or reversed.

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