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公开(公告)号：US12110534B2
公开(公告)日：2024-10-08
申请号：US16945099
申请日：2020-07-31
Applicant: Roche Sequencing Solutions, Inc. , Kapa Biosystems, Inc.
Inventor： Daleen Badenhorst , Michael S. Berry , Richard Dannebaum , Ashley Hayes , Severine Margeridon , Martin Ranik , Etienne Slabbert
IPC: C12Q1/6806 , C12Q1/6869
CPC classification number: C12Q1/6806 , C12Q1/6869 , C12Q2521/319 , C12Q2525/155 , C12Q2525/191 , C12Q2525/307 , C12Q2535/122 , C12Q1/6869 , C12Q2525/307 , C12Q2535/122 , C12Q1/6806 , C12Q2525/191 , C12Q2525/155 , C12Q2521/319
Abstract: The invention is a novel method of sequencing nucleic acids involving making and sequencing a library of single stranded circular target nucleic acids.
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公开(公告)号：US12084724B2
公开(公告)日：2024-09-10
申请号：US17494669
申请日：2021-10-05
Applicant: Inivata Ltd.
Inventor： Samuel Woodhouse , Stefanie Lensing , Tim Forshew , Vincent Plagnol , Matthew Edward Smith , Karen Howarth , Michael Epstein
IPC: C12Q1/6886 , C12Q1/6806 , C12Q1/6853 , C12Q1/686 , G16B20/00 , G16B30/00
CPC classification number: C12Q1/6886 , C12Q1/6806 , C12Q1/6853 , C12Q1/686 , C12Q2600/106 , C12Q2600/156 , C12Q2600/16 , G16B20/00 , G16B30/00 , C12Q1/6853 , C12Q2525/155 , C12Q2525/161 , C12Q2531/113 , C12Q2535/122 , C12Q2537/159
Abstract: The present disclosure relates to methods for detecting and targeting genomic rearrangements, in particular gene fusion events, by targeting a DNA molecule of interest with a set or pool of primers, wherein the forward primers and reverse primers produce a PCR amplification product when a genomic rearrangement is present. The present disclosure also relates to methods of bioinformatic analysis to determine whether or not the detection of an amplification product from the selective PCR is actually indicative of the presence of a gene fusion. The present disclosure also related to related methods of diagnosis and treatment of diseases and conditions associated with such genomic rearrangements, in particular cancers, such as lung cancer.
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公开(公告)号：US12054768B2
公开(公告)日：2024-08-06
申请号：US17474424
申请日：2021-09-14
Applicant: The Regents of the University of California
Inventor： Peter Robinson , Cheng-Ting Tsai , Carolyn Bertozzi
IPC: C12Q1/6804 , C12Q1/6806 , C12Q1/686 , G01N33/543
CPC classification number: C12Q1/6804 , C12Q1/6806 , C12Q1/686 , G01N33/54306 , C12Q2525/117 , C12Q2525/155 , C12Q2525/197 , C12Q2531/113
Abstract: Methods are provided for detecting antigen binding agents in samples. Aspects of the methods include detection of the aggregation of antigen binding agents with polynucleotide-bound antigens by sensitive proximity-based association of the antigen-bound polynucleotides. Aspects of the methods also include methods for the detection of such proximity-based association through nucleic acid amplification. In addition, compositions, e.g., reagents, kits, and devices, useful in practicing various embodiments of the methods are provided.
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公开(公告)号：US11834699B2
公开(公告)日：2023-12-05
申请号：US16372186
申请日：2019-04-01
Applicant: ILLUMINA CAMBRIDGE LIMITED
Inventor： Niall Anthony Gormley , Louise Fraser , Paula Kokko-Gonzales
IPC: C12Q1/6806 , C12Q1/6834 , C12Q1/6869
CPC classification number: C12Q1/6806 , C12Q1/6834 , C12Q1/6869 , C12Q1/6806 , C12Q2525/155 , C12Q2525/191 , C12Q2535/122 , C12Q2565/518 , C12Q1/6869 , C12Q2525/155 , C12Q2525/191 , C12Q2535/122 , C12Q2565/518 , C12Q1/6834 , C12Q2525/155 , C12Q2525/191 , C12Q2535/122 , C12Q2565/518
Abstract: Provided herein is a method of using transposition to improve methods of sequencing RNA molecules. Provided herein is a method of tagging nucleic acid duplexes, such as DNA:RNA duplexes or DNA:DNA duplexes. The method includes the steps of providing a transposase and a transposon composition, providing one or more nucleic acid duplexes immobilized on a support, and contacting the transposase and transposon composition with the one or more nucleic acid duplexes under conditions wherein the one or more nucleic acid duplexes and transposon composition undergo a transposition reaction to produce one or more tagged nucleic acid duplexes, wherein the transposon composition comprises a double stranded nucleic acid molecule comprising a transferred strand and a non-transferred strand.
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公开(公告)号：US20190241977A1
公开(公告)日：2019-08-08
申请号：US16389588
申请日：2019-04-19
Applicant: Inivata Ltd.
Inventor： Samuel Woodhouse , Stefanie Lensing , Tim Forshew , Vincent Plagnol , Matthew Edward Smith , Karen Howarth , Michael Epstein
IPC: C12Q1/6886 , C12Q1/6853
CPC classification number: C12Q1/6886 , C12Q1/6806 , C12Q1/6853 , C12Q1/686 , C12Q2600/106 , C12Q2600/16 , G16B20/00 , G16B30/00 , C12Q2525/155 , C12Q2525/161 , C12Q2531/113 , C12Q2535/122 , C12Q2537/159
Abstract: The present disclosure relates to methods for detecting and targeting genomic rearrangements, in particular gene fusion events, by targeting a DNA molecule of interest with a set or pool of primers, wherein the forward primers and reverse primers produce a PCR amplification product when a genomic rearrangement is present. The present disclosure also relates to methods of bioinformatic analysis to determine whether or not the detection of an amplification product from the selective PCR is actually indicative of the presence of a gene fusion. The present disclosure also related to related methods of diagnosis and treatment of diseases and conditions associated with such genomic rearrangements, in particular cancers, such as lung cancer.
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公开(公告)号：US20190241976A1
公开(公告)日：2019-08-08
申请号：US16389584
申请日：2019-04-19
Applicant: Inivata Ltd.
Inventor： Samuel Woodhouse , Stefanie Lensing , Tim Forshew , Vincent Plagnol , Matthew Edward Smith , Karen Howarth , Michael Epstein
IPC: C12Q1/6886 , C12Q1/6853
CPC classification number: C12Q1/6886 , C12Q1/6806 , C12Q1/6853 , C12Q1/686 , C12Q2600/106 , C12Q2600/16 , G16B20/00 , G16B30/00 , C12Q2525/155 , C12Q2525/161 , C12Q2531/113 , C12Q2535/122 , C12Q2537/159
Abstract: The present disclosure relates to methods for detecting and targeting genomic rearrangements, in particular gene fusion events, by targeting a DNA molecule of interest with a set or pool of primers, wherein the forward primers and reverse primers produce a PCR amplification product when a genomic rearrangement is present. The present disclosure also relates to methods of bioinformatic analysis to determine whether or not the detection of an amplification product from the selective PCR is actually indicative of the presence of a gene fusion. The present disclosure also related to related methods of diagnosis and treatment of diseases and conditions associated with such genomic rearrangements, in particular cancers, such as lung cancer.
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公开(公告)号：US20190241975A1
公开(公告)日：2019-08-08
申请号：US16389573
申请日：2019-04-19
Applicant: Inivata Ltd.
Inventor： Samuel Woodhouse , Stefanie Lensing , Tim Forshew , Vincent Plagnol , Matthew Edward Smith , Karen Howarth , Michael Epstein
IPC: C12Q1/6886 , C12Q1/6853
CPC classification number: C12Q1/6886 , C12Q1/6806 , C12Q1/6853 , C12Q1/686 , C12Q2600/106 , C12Q2600/16 , G16B20/00 , G16B30/00 , C12Q2525/155 , C12Q2525/161 , C12Q2531/113 , C12Q2535/122 , C12Q2537/159
Abstract: The present disclosure relates to methods for detecting and targeting genomic rearrangements, in particular gene fusion events, by targeting a DNA molecule of interest with a set or pool of primers, wherein the forward primers and reverse primers produce a PCR amplification product when a genomic rearrangement is present. The present disclosure also relates to methods of bioinformatic analysis to determine whether or not the detection of an amplification product from the selective PCR is actually indicative of the presence of a gene fusion. The present disclosure also related to related methods of diagnosis and treatment of diseases and conditions associated with such genomic rearrangements, in particular cancers, such as lung cancer.
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公开(公告)号：US20190241974A1
公开(公告)日：2019-08-08
申请号：US16389565
申请日：2019-04-19
Applicant: Inivata Ltd.
Inventor： Samuel Woodhouse , Stefanie Lensing , Tim Forshew , Vincent Plagnol , Matthew Edward Smith , Karen Howarth , Michael Epstein
IPC: C12Q1/6886 , C12Q1/6806 , C12Q1/686
CPC classification number: C12Q1/6886 , C12Q1/6806 , C12Q1/6853 , C12Q1/686 , C12Q2600/106 , C12Q2600/16 , G16B20/00 , G16B30/00 , C12Q2525/155 , C12Q2525/161 , C12Q2531/113 , C12Q2535/122 , C12Q2537/159
Abstract: The present disclosure relates to methods for detecting and targeting genomic rearrangements, in particular gene fusion events, by targeting a DNA molecule of interest with a set or pool of primers, wherein the forward primers and reverse primers produce a PCR amplification product when a genomic rearrangement is present. The present disclosure also relates to methods of bioinformatic analysis to determine whether or not the detection of an amplification product from the selective PCR is actually indicative of the presence of a gene fusion. The present disclosure also related to related methods of diagnosis and treatment of diseases and conditions associated with such genomic rearrangements, in particular cancers, such as lung cancer.
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公开(公告)号：US20190203278A1
公开(公告)日：2019-07-04
申请号：US16190625
申请日：2018-11-14
Applicant: Theranos IP Company, LLC
Inventor： Kamila Belhocine , Josephine Lee , Pranav Patel , Aaron Richardson , Scott Tabakman
IPC: C12Q1/6844 , C07H1/00 , C12P19/34 , C12Q1/686
CPC classification number: C12Q1/6844 , C07H1/00 , C12P19/34 , C12Q1/686 , C12Q2525/151 , C12Q2525/155 , C12Q2525/161 , C12Q2527/101 , C12Q2531/119
Abstract: Methods and compositions for the amplification of nucleic acids and generation of concatemers are disclosed. Amplification methods provided herein may be performed under isothermal conditions. Methods and compositions may include reagents such as nucleic acid polymerases and primers.
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公开(公告)号：US20190194722A1
公开(公告)日：2019-06-27
申请号：US16304114
申请日：2017-05-25
Applicant: Oxford Nanopore Technologies Ltd.
Inventor： David Jackson Stoddart , James White
IPC: C12Q1/6806 , C12Q1/6869
CPC classification number: C12Q1/6806 , C12Q1/6869 , C12Q2521/513 , C12Q2525/155 , C12Q2525/191 , C12Q2535/122 , C12Q2565/631
Abstract: The invention relates to a method for modifying a template double stranded polynucleotide, especially for characterisation using nanopore sequencing. The method produces from the template a plurality of modified double stranded polynucleotides. These modified polynucleotides can then be characterised.
