公开(公告)号：US12129494B2

公开(公告)日：2024-10-29

申请号：US17558454

申请日：2021-12-21

Applicant: Codexis, Inc.

Inventor： Mathew G. Miller ,  Chinping Chng ,  Oscar Alvizo ,  Melissa Ann Mayo ,  James Nicholas Riggins ,  Xiang Yi ,  Jonathan S. Penfield ,  Gjalt W. Huisman ,  Jared Davis ,  Yasushi Saotome

IPC: C12N9/12 ,  C12P19/34 ,  C12N15/00

CPC classification number: C12N9/1247 ,  C12P19/34 ,  C12Y207/07006 ,  C12N15/00

Abstract: The present invention provides engineered RNA polymerase variants and compositions comprising these variants. The present invention further provides engineered T7 RNA polymerase variants and compositions comprising these variants. These variants have been evolved for selective incorporation of the m7G(5′)ppp(5′)m7G cap analog over GTP at the initiation of in vitro transcription. The present invention also provides methods for selective capping of RNA transcripts.

公开(公告)号：US12091685B2

公开(公告)日：2024-09-17

申请号：US17742033

申请日：2022-05-11

Applicant: New England Biolabs, Inc.

Inventor： Jennifer Ong ,  Vladimir Potapov ,  Kuo-Chan Hung ,  Haruichi Asahara ,  Shaorong Chong ,  George Tzertzinis

IPC: C12N9/12 ,  C12Q1/6858

CPC classification number: C12N9/1247 ,  C12Q1/6858 ,  C12Y207/07006

Abstract: A bacteriophage RNA polymerase variant is provided. In some embodiments, the variant may have increased thermostability relative to the corresponding wild type bacteriophage RNA polymerase and/or wild type T7 RNA polymerase. Compositions, kits and methods that employ the variant are also provided.

公开(公告)号：US20240294979A1

公开(公告)日：2024-09-05

申请号：US18486005

申请日：2023-10-12

Applicant: Karius, Inc.

Inventor： Timothy A. Blauwkamp ,  Rene Sit ,  Igor D. Vilfan

IPC: C12Q1/6869 ,  C12N9/00 ,  C12N9/12 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6876

CPC classification number: C12Q1/6869 ,  C12N9/1247 ,  C12N9/93 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6876 ,  C12Q2521/107 ,  C12Q2533/101 ,  C12Y207/07006 ,  C12Y207/07049 ,  C12Y605/01001 ,  C12Y605/01003

Abstract: The disclosure provides methods, compositions, systems, and kits for the concurrent detection and analysis of different structural and chemical forms of nucleic acids in a sample.

公开(公告)号：US11827885B1

公开(公告)日：2023-11-28

申请号：US17207507

申请日：2021-03-19

Applicant: New England Biolabs, Inc.

Inventor： Nathan Tanner ,  Jennifer Ong ,  Esta Slayton ,  Lisa Maduzia ,  Salvatore V. Russello

IPC: C12N15/11 ,  C12N15/113 ,  C12N15/10 ,  C12N9/12 ,  C12Q1/6848 ,  C12N9/58

CPC classification number: C12N15/1137 ,  C12N9/1247 ,  C12N9/1276 ,  C12N15/1003 ,  C12Q1/6848 ,  C12N9/58 ,  C12N2310/16 ,  C12Y207/07006 ,  C12Y207/07049 ,  C12Q1/6848 ,  C12Q2525/205 ,  C12Q2527/127

Abstract: Compositions, methods and kits are provided that include an inhibitory oligonucleotide RNase inhibitor capable of inhibiting one or more types of RNase that coexist with biological samples or are introduced in the laboratory, thereby protecting RNA in the sample from degradation. More than one type of oligonucleotide RNase inhibitor may be combined in a mixture to inhibit a plurality of different RNases. Single oligonucleotides were identified to have inhibitory activity for a plurality of different RNases. The RNase oligonucleotide inhibitor may be immobilized on beads or other surface. It may be stored in a lyophilized form or in solution.

公开(公告)号：US20230313156A1

公开(公告)日：2023-10-05

申请号：US18019847

申请日：2021-05-13

Applicant: Tsinghua University

Inventor： Ting ZHU ,  Chuyao FAN ,  Qiang DENG ,  Yuan XU

IPC: C12N9/12 ,  C07K1/02 ,  C12P19/34

CPC classification number: C12N9/1252 ,  C07K1/026 ,  C12P19/34 ,  C12Y207/07007 ,  C12N9/1247 ,  C12Y207/07006

Abstract: Provided herein is a general method for producing large (more than 400 aa long) D-amino acids proteins, also referred to as mirror image protein (with respect to their naturally occurring L-amino acids counterparts), including RNA/DNA manipulating enzymes, and uses thereof in a wide range of research, practical data storage and medicinal applications.

公开(公告)号：US11760986B2

公开(公告)日：2023-09-19

申请号：US17123632

申请日：2020-12-16

Applicant: President and Fellows of Harvard College

Inventor： David R. Liu ,  Bryan Dickinson ,  Michael S. Packer ,  Ahmed Hussein Badran

IPC: C12N15/10 ,  C12N9/50 ,  C12N15/73 ,  C12N7/00 ,  C12N9/12 ,  C12N15/52 ,  C12N15/62 ,  C12N15/86

CPC classification number: C12N9/50 ,  C12N7/00 ,  C12N9/1247 ,  C12N15/1037 ,  C12N15/1058 ,  C12N15/52 ,  C12N15/62 ,  C12N15/73 ,  C12N15/86 ,  C12Y207/07006 ,  C12Y304/00 ,  C07K2319/50 ,  C12N2795/00022

Abstract: Some aspects of this disclosure provide methods for phage-assisted continuous evolution (PACE) of proteases. Some aspects of this invention provide methods for evaluating and selecting protease inhibitors based on the likelihood of the emergence of resistant proteases as determined by the protease PACE methods provided herein. Some aspects of this disclosure provide strategies, methods, and reagents for protease PACE, including fusion proteins for translating a desired protease activity into a selective advantage for phage particles encoding a protease exhibiting such an activity and improved mutagenesis-promoting expression constructs. Evolved proteases that recognize target cleavage sites which differ from their canonical cleavage site are also provided herein.

公开(公告)号：US20230235373A1

公开(公告)日：2023-07-27

申请号：US18009673

申请日：2021-06-11

Applicant: SYNHELIX

Inventor： Irina RANDRIANJATOVO-GBALOU ,  Ahmed SAID ,  Renaud RAHIER

IPC: C12P19/34 ,  C12N9/12

CPC classification number: C12P19/34 ,  C12N9/1247 ,  C12Y207/07006

Abstract: The invention relates to methods for template-independent synthesis of nucleic acids, comprising iteratively contacting an initiator sequence comprising a 3′-end nucleotide with a free 3′-hydroxyl group, with at least one nucleoside triphosphate, or a combination of nucleoside triphosphates, in the presence of an archaeal DNA primase or a functionally active fragment and/or variant thereof, thereby covalently binding said nucleoside triphosphate to the free 3-hydroxyl group of the 3-end nucleotide. It also relates to isolated functionally active fragments of archaeal DNA primases which are capable of template-independent terminal nucleotidyl transferase activity but are devoid of a template-independent primase activity.

公开(公告)号：US20180258423A1

公开(公告)日：2018-09-13

申请号：US15907163

申请日：2018-02-27

Applicant: Translate Bio, Inc.

Inventor： Anusha Dias ,  Daniel Crawford ,  Frank DeRosa ,  Jonathan Abysalh ,  Michael Heartlein

IPC: C12N15/10 ,  G01N27/447

CPC classification number: C12N15/1096 ,  C12N9/1247 ,  C12N15/1068 ,  C12P19/34 ,  C12Y207/07006 ,  G01N27/44778

Abstract: The present invention provides methods for large-scale production of a composition enriched for full-length mRNA molecules using an SP6 RNA polymerase and compositions produced using such methods and uses thereof.

公开(公告)号：US09758771B2

公开(公告)日：2017-09-12

申请号：US14392186

申请日：2014-06-24

Applicant: CJ CHEILJEDANG CORPORATION

Inventor： Sang Hee Park ,  Jun Ok Moon ,  Kwang Ho Lee

IPC: C12N9/12 ,  C12P13/08 ,  C12N15/77

CPC classification number: C12N9/1247 ,  C12N15/77 ,  C12P13/08 ,  C12Y207/07006

Abstract: Provided are a beta prime subunit mutant of RNA polymerase, a microorganism of the Corynebacterium genus including a polynucleotide coding the same, and a method for producing L-lysine by culturing the same.

公开(公告)号：US20160281070A1

公开(公告)日：2016-09-29

申请号：US14899676

申请日：2014-04-24

Applicant: CJ Cheiljedang Corporation

Inventor： Ji Sun Lee ,  Kwang Ho Lee ,  Eun Sung Koh ,  Hyung Joon Kim ,  Keun Cheol Lee ,  Young Bin Hwang

IPC: C12N9/12 ,  C12P13/08

CPC classification number: C12N9/1247 ,  C07K14/245 ,  C12N15/70 ,  C12P13/08 ,  C12Y207/07006

Abstract: The present application relates to an L-threonine-producing microorganism and a production method for L-threonine using the same, and more specifically, to a microorganism having enhanced L-threonine productivity and a method for producing L-threonine in high yield using the same.

Abstract translation: 本申请涉及L-苏氨酸生产微生物和使用其的L-苏氨酸的制备方法，更具体地说，涉及具有增强的L-苏氨酸生产力的微生物和使用该产物的高产率L-赖氨酸生产方法 相同。