公开(公告)号：US20240360488A1

公开(公告)日：2024-10-31

申请号：US18429780

申请日：2024-02-01

Applicant: 4BASEBIO, S.L.U. ,  4BASEBIO UK LTD

Inventor： Heikki Lanckriet ,  Amy Walker ,  Ángel Picher

IPC: C12P19/34 ,  C12N9/22 ,  C12N15/10

CPC classification number: C12P19/34 ,  C12N9/22 ,  C12N15/10 ,  C12N2310/122 ,  C12N2310/315 ,  C12Y207/07 ,  C12Y301/11001 ,  C12Y301/11002

Abstract: Methods for producing a linear deoxyribonucleic acid (DNA) product with enhanced resistance to nuclease digestion are provided. The methods comprise, (a) digesting a double-stranded DNA molecule with an endonuclease that cleaves an endonuclease target sequence to generate a digested double-stranded DNA molecule, wherein the digested double-stranded DNA molecule comprises a linear double-stranded region, and a truncated protelomerase sequence at a first end, wherein the truncated protelomerase target sequence is non-functional; (b) appending a first adaptor molecule to the first end of the digested double-stranded DNA molecule and appending a second adaptor molecule to the second end of the digested double-stranded DNA molecule to generate a precursor double-stranded DNA molecule, wherein the first adaptor molecule comprises a truncated protelomerase target sequence that forms a first functional protelomerase target sequence with the truncated protelomerase sequence at the first end of the digested double-stranded DNA molecule; and (c) incubating the precursor double-stranded DNA molecule with a protelomerase to generate the linear DNA product, wherein the protelomerase closes the first end of the precursor double-stranded DNA molecule at the first functional protelomerase target sequence.

公开(公告)号：US12129494B2

公开(公告)日：2024-10-29

申请号：US17558454

申请日：2021-12-21

Applicant: Codexis, Inc.

Inventor： Mathew G. Miller ,  Chinping Chng ,  Oscar Alvizo ,  Melissa Ann Mayo ,  James Nicholas Riggins ,  Xiang Yi ,  Jonathan S. Penfield ,  Gjalt W. Huisman ,  Jared Davis ,  Yasushi Saotome

IPC: C12N9/12 ,  C12P19/34 ,  C12N15/00

CPC classification number: C12N9/1247 ,  C12P19/34 ,  C12Y207/07006 ,  C12N15/00

Abstract: The present invention provides engineered RNA polymerase variants and compositions comprising these variants. The present invention further provides engineered T7 RNA polymerase variants and compositions comprising these variants. These variants have been evolved for selective incorporation of the m7G(5′)ppp(5′)m7G cap analog over GTP at the initiation of in vitro transcription. The present invention also provides methods for selective capping of RNA transcripts.

公开(公告)号：US20240352496A1

公开(公告)日：2024-10-24

申请号：US18687892

申请日：2022-08-29

Applicant: GLAXOSMITHKLINE BIOLOGICALS SA

Inventor： Justin Ross HALMAN ,  Nilesh VAIDYA

IPC: C12P19/34

CPC classification number: C12P19/34

Abstract: Improved methods for manufacturing a RNA by IVT and compositions for use therein are provided.

公开(公告)号：US20240352495A1

公开(公告)日：2024-10-24

申请号：US18685259

申请日：2022-09-13

Applicant: Moderna Enzymatics Co., Ltd.

Inventor： Masayuki Suetsugu ,  Seia Nara

IPC: C12P19/34 ,  C12N9/12 ,  C12N15/11

CPC classification number: C12P19/34 ,  C12N9/1205 ,  C12N9/1217 ,  C12N9/1223 ,  C12N9/1229 ,  C12N15/11 ,  C12Y207/0104 ,  C12Y207/02001 ,  C12Y207/03002 ,  C12Y207/04001 ,  C12Y207/04006

Abstract: Provided is a method for producing circular DNA in which a region that is sandwiched by a region Ha and a region Hb in circular double-stranded DNA is substituted with the entirety or a portion of a linear-DNA fragment, wherein the region Hb is located downstream of the region Ha in the circular double-stranded DNA; the linear-DNA fragment is single-stranded or double-stranded linear DNA that has a homologous region that corresponds to the region Ha and a homologous region that corresponds to the region Hb, the latter homologous region being positioned downstream of the former homologous region; and the method comprising: preparing a reaction solution that contains the circular double-stranded DNA, the linear-DNA fragment, and a protein that has RecA-family-recombinase activity, and performing homologous recombination reaction by incubating the reaction solution for a predetermined period of time, thereby producing circular DNA in which the region that is from the region Ha to the region Hb in the circular double-stranded DNA is substituted with the region that is from the homologous region that corresponds to the region Ha to the homologous region that corresponds to the region Hb in the linear-DNA fragment.

公开(公告)号：US20240336945A1

公开(公告)日：2024-10-10

申请号：US18292098

申请日：2022-07-26

Applicant: Flagship Pioneering Innovations VI, LLC

Inventor： David Arthur Berry ,  Theonie Anastassiadis ,  Christine Elizabeth Hajdin ,  Noubar Boghos Afeyan

IPC: C12P19/34 ,  A61K31/7105 ,  C12N15/10

CPC classification number: C12P19/34 ,  A61K31/7105 ,  C12N15/1003

Abstract: The invention relates generally to tRNA-based effector molecules and methods relating thereto.

公开(公告)号：US20240287582A1

公开(公告)日：2024-08-29

申请号：US18652923

申请日：2024-05-02

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Je-hyuk Lee ,  Richard C. Terry ,  Evan R. Daugharthy

IPC: C12Q1/6806 ,  C12P19/34 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12Q1/6874

CPC classification number: C12Q1/6806 ,  C12P19/34 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12Q1/6874

Abstract: Methods of making a three-dimensional matrix of nucleic acids within a cell is provided.

公开(公告)号：US12060613B2

公开(公告)日：2024-08-13

申请号：US17219666

申请日：2021-03-31

Applicant: EARLI Inc.

Inventor： David Suhy ,  Paul Escarpe ,  Cyriac Roeding ,  Justin Lin ,  Alex Harwig ,  Shireen Rudina ,  Leland Harrison Hartwell

IPC: C12Q1/68 ,  C12Q1/28 ,  C12Q1/42 ,  C12Q1/66 ,  C12Q1/6883 ,  C12Q1/6886

CPC classification number: C12Q1/6883 ,  C12Q1/28 ,  C12Q1/42 ,  C12Q1/66 ,  C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/158 ,  C12Q2600/178 ,  C12Q1/68 ,  C12P19/34

Abstract: The present disclosure encompasses embodiments of nucleic acids comprising genetic elements which are useful for the detection of diseased cells.

公开(公告)号：US12054734B2

公开(公告)日：2024-08-06

申请号：US17338795

申请日：2021-06-04

Applicant: The Trustees of the University of Pennsylvania

Inventor： Drew Weissman ,  Katalin Kariko

IPC: C12N15/85 ,  C07H21/02 ,  C07K14/475 ,  C07K14/505 ,  C07K14/52 ,  C12P19/34 ,  A61K48/00

CPC classification number: C12N15/85 ,  C07H21/02 ,  C07K14/475 ,  C07K14/505 ,  C07K14/52 ,  C12P19/34 ,  A61K48/00 ,  C12N2310/335 ,  C12N2320/30

Abstract: This invention provides purified preparations of an RNA, oligoribonucleotide, or polyribonucleotide comprising a modified nucleoside, and methods of assessing purity of purified preparations of an RNA, oligoribonucleotide, or polyribonucleotide comprising a modified nucleoside.

公开(公告)号：US20240254546A1

公开(公告)日：2024-08-01

申请号：US18487416

申请日：2023-10-16

Applicant: AMPLIWISE INC.

Inventor： Kai Wu ,  Mindy Su ,  Xing Su

IPC: C12Q1/6853 ,  C12P19/34 ,  C12Q1/6827 ,  C12Q1/686

CPC classification number: C12Q1/6853 ,  C12P19/34 ,  C12Q1/6827 ,  C12Q1/686

Abstract: An embodiment relates to system comprising: (i) a reaction mixture comprises: a) a target nucleic acid molecule; b) a forward primer complementary to a strand of the target nucleic acid molecule, the forward primer comprises a first molecular moiety at a 3′ end, wherein the first molecular moiety is non-complementary to the strand of the target nucleic acid molecule; c) a reverse primer complementary to a complementary sequence of the strand of the target nucleic acid molecule, the reverse primer comprises a second molecular moiety at a 3′ end, wherein the second molecular moiety is non-complementary to the complementary sequence of the strand of the target nucleic acid molecule; d) a polymerase with 3′-5′ exonuclease activity; and (ii a device suitable of detecting an amplification products.

公开(公告)号：US20240252622A1

公开(公告)日：2024-08-01

申请号：US18615472

申请日：2024-03-25

Applicant: INSTITUT PASTEUR

Inventor： Etienne SIMON-LORIERE ,  Matthieu PROT ,  Xavier MONTAGUTELLI

IPC: A61K39/215 ,  A61K39/00 ,  C12N15/63 ,  C12P19/34

CPC classification number: A61K39/215 ,  C12N15/63 ,  C12P19/34 ,  A61K2039/51

Abstract: The invention relates to an immunogenic or vaccine composition against the 2019 novel coronavirus (SARS-COV-2), comprising a nucleic acid construct encoding a SARS-COV-2 coronavirus Spike (S) protein antigen or a fragment thereof comprising the receptor-binding domain, wherein the nucleic acid construct sequence is codon-optimized for expression in 5 human.