公开(公告)号：US12129494B2

公开(公告)日：2024-10-29

申请号：US17558454

申请日：2021-12-21

申请人： Codexis, Inc.

发明人： Mathew G. Miller ,  Chinping Chng ,  Oscar Alvizo ,  Melissa Ann Mayo ,  James Nicholas Riggins ,  Xiang Yi ,  Jonathan S. Penfield ,  Gjalt W. Huisman ,  Jared Davis ,  Yasushi Saotome

IPC分类号： C12N9/12 ,  C12P19/34 ,  C12N15/00

CPC分类号： C12N9/1247 ,  C12P19/34 ,  C12Y207/07006 ,  C12N15/00

摘要： The present invention provides engineered RNA polymerase variants and compositions comprising these variants. The present invention further provides engineered T7 RNA polymerase variants and compositions comprising these variants. These variants have been evolved for selective incorporation of the m7G(5′)ppp(5′)m7G cap analog over GTP at the initiation of in vitro transcription. The present invention also provides methods for selective capping of RNA transcripts.

公开(公告)号：US11236311B2

公开(公告)日：2022-02-01

申请号：US16938793

申请日：2020-07-24

申请人： Codexis, Inc.

发明人： Mathew G. Miller ,  Chinping Chng ,  Oscar Alvizo ,  Melissa Ann Mayo ,  James Nicholas Riggins ,  Xiang Yi ,  Jonathan S. Penfield ,  Gjalt W. Huisman ,  Jared Davis ,  Yasushi Saotome

IPC分类号： C12N9/12 ,  C12N1/20 ,  C12N15/00 ,  C12P19/34

摘要： The present invention provides engineered RNA polymerase variants and compositions comprising these variants. The present invention further provides engineered T7 RNA polymerase variants and compositions comprising these variants. These variants have been evolved for selective incorporation of the m7G(5′)ppp(5′)m7G cap analog over GTP at the initiation of in vitro transcription. The present invention also provides methods for selective capping of RNA transcripts.

公开(公告)号：US11236312B2

公开(公告)日：2022-02-01

申请号：US16942599

申请日：2020-07-29

申请人： Codexis, Inc.

发明人： Mathew G. Miller ,  Chinping Chng ,  Oscar Alvizo ,  Melissa Ann Mayo ,  James Nicholas Riggins ,  Xiang Yi ,  Jonathan S. Penfield ,  Gjalt W. Huisman ,  Jared Davis ,  Yasushi Saotome

IPC分类号： C12N9/12 ,  C12P19/34 ,  C12N15/00

摘要： The present invention provides engineered RNA polymerase variants and compositions comprising these variants. The present invention further provides engineered T7 RNA polymerase variants and compositions comprising these variants. These variants have been evolved for selective incorporation of the m7G(5′)ppp(5′)m7G cap analog over GTP at the initiation of in vitro transcription. The present invention also provides methods for selective capping of RNA transcripts.

公开(公告)号：US10738286B2

公开(公告)日：2020-08-11

申请号：US16012462

申请日：2018-06-19

申请人： Codexis, Inc.

发明人： Mathew G. Miller ,  Chinping Chng ,  Oscar Alvizo ,  Melissa Ann Mayo ,  James Nicholas Riggins ,  Xiang Yi ,  Jonathan S. Penfield ,  Gjalt W. Huisman ,  Jared Davis ,  Yasushi Saotome

IPC分类号： C12N9/12 ,  C12P19/34 ,  C12N15/00 ,  C12P21/06 ,  C12P21/04

摘要： The present invention provides engineered RNA polymerase variants and compositions comprising these variants. The present invention further provides engineered T7 RNA polymerase variants and compositions comprising these variants. These variants have been evolved for selective incorporation of the m7G(5′)ppp(5′)m7G cap analog over GTP at the initiation of in vitro transcription. The present invention also provides methods for selective capping of RNA transcripts.

公开(公告)号：US20220186197A1

公开(公告)日：2022-06-16

申请号：US17558454

申请日：2021-12-21

申请人： Codexis, Inc.

发明人： Mathew G. Miller ,  Chinping Chng ,  Oscar Alvizo ,  Melissa Ann Mayo ,  James Nicholas Riggins ,  Xiang Yi ,  Jonathan S. Penfield ,  Gjalt W. Huisman ,  Jared Davis ,  Yasushi Saotome

IPC分类号： C12N9/12 ,  C12P19/34

摘要： The present invention provides engineered RNA polymerase variants and compositions comprising these variants. The present invention further provides engineered T7 RNA polymerase variants and compositions comprising these variants. These variants have been evolved for selective incorporation of the m7G(5′)ppp(5′)m7G cap analog over GTP at the initiation of in vitro transcription. The present invention also provides methods for selective capping of RNA transcripts.

公开(公告)号：US20190002850A1

公开(公告)日：2019-01-03

申请号：US16012462

申请日：2018-06-19

申请人： Codexis, Inc. ,  Alexion Pharmaceuticals, Inc.

发明人： Mathew G. Miller ,  Chinping Chng ,  Oscar Alvizo ,  Melissa Ann Mayo ,  James Nicholas Riggins ,  Xiang Yi ,  Jonathan S. Penfield ,  Gjalt W. Huisman ,  Jared Davis ,  Yasushi Saotome

IPC分类号： C12N9/12 ,  C12P19/34

摘要： The present invention provides engineered RNA polymerase variants and compositions comprising these variants. The present invention further provides engineered T7 RNA polymerase variants and compositions comprising these variants. These variants have been evolved for selective incorporation of the m7G(5′)ppp(5′)m7G cap analog over GTP at the initiation of in vitro transcription. The present invention also provides methods for selective capping of RNA transcripts.