公开(公告)号：US20240352495A1

公开(公告)日：2024-10-24

申请号：US18685259

申请日：2022-09-13

申请人： Moderna Enzymatics Co., Ltd.

发明人： Masayuki Suetsugu ,  Seia Nara

IPC分类号： C12P19/34 ,  C12N9/12 ,  C12N15/11

CPC分类号： C12P19/34 ,  C12N9/1205 ,  C12N9/1217 ,  C12N9/1223 ,  C12N9/1229 ,  C12N15/11 ,  C12Y207/0104 ,  C12Y207/02001 ,  C12Y207/03002 ,  C12Y207/04001 ,  C12Y207/04006

摘要： Provided is a method for producing circular DNA in which a region that is sandwiched by a region Ha and a region Hb in circular double-stranded DNA is substituted with the entirety or a portion of a linear-DNA fragment, wherein the region Hb is located downstream of the region Ha in the circular double-stranded DNA; the linear-DNA fragment is single-stranded or double-stranded linear DNA that has a homologous region that corresponds to the region Ha and a homologous region that corresponds to the region Hb, the latter homologous region being positioned downstream of the former homologous region; and the method comprising: preparing a reaction solution that contains the circular double-stranded DNA, the linear-DNA fragment, and a protein that has RecA-family-recombinase activity, and performing homologous recombination reaction by incubating the reaction solution for a predetermined period of time, thereby producing circular DNA in which the region that is from the region Ha to the region Hb in the circular double-stranded DNA is substituted with the region that is from the homologous region that corresponds to the region Ha to the homologous region that corresponds to the region Hb in the linear-DNA fragment.

公开(公告)号：US20180208909A1

公开(公告)日：2018-07-26

申请号：US15746300

申请日：2016-07-19

申请人： CJ Cheiljedang Corporation

发明人： Su Jin PARK ,  Young Lyeol YANG ,  Hye Won UM ,  Hong Xian LI ,  Kyoung Min LEE ,  Baek Seok LEE ,  Hyo Hyoung LEE ,  Hee Kyoung JUNG

IPC分类号： C12N9/10 ,  C12N9/80 ,  C12P13/00 ,  C12P13/10 ,  C12N9/00 ,  C12N9/88 ,  C12N9/12

CPC分类号： C12N9/1029 ,  C12N9/1217 ,  C12N9/80 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12P13/001 ,  C12P13/10 ,  C12Y203/01001 ,  C12Y203/01008 ,  C12Y207/02001 ,  C12Y305/01016 ,  C12Y401/01017 ,  C12Y602/01001

摘要： Disclosed is a modified microorganism producing putrescine or ornithine, and a method for producing putrescine or ornithine using the same.

公开(公告)号：US09957531B1

公开(公告)日：2018-05-01

申请号：US15658668

申请日：2017-07-25

申请人： LanzaTech New Zealand Limited

发明人： Michael Koepke ,  Rasmus Overgaard Jensen ,  James Bruce Yarnton Haycock Behrendorff ,  Ryan Edward Hill ,  Darmawi Juminaga ,  Alexander Paul Mueller

IPC分类号： C12N1/20 ,  C12P7/42 ,  C12N9/10 ,  C12N9/02 ,  C12N9/04 ,  C12N9/12 ,  C12N9/16 ,  C12P7/24 ,  C12P7/18

CPC分类号： C12P7/42 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/1217 ,  C12N9/16 ,  C12P5/007 ,  C12P5/026 ,  C12P7/04 ,  C12P7/18 ,  C12P7/24 ,  C12P7/28 ,  C12P7/625 ,  C12Y101/01001 ,  C12Y101/01002 ,  C12Y102/07005 ,  C12Y203/01008 ,  C12Y203/01009 ,  C12Y203/01019 ,  C12Y207/02001 ,  C12Y207/02007 ,  C12Y301/0202

摘要： The invention relates to a genetically engineered bacterium having an enzyme that converts acetyl-CoA to acetoacetyl-CoA, an enzyme that converts acetoacetyl-CoA to 3-hydroxybutyryl-CoA, and an enzyme that converts 3-hydroxybutyryl-CoA to 3-hydroxybutyrate. The bacterium may also have enzymes to produce other downstream products, such as 3-hydroxybutyryaldehyde, and 1,3-butanediol. Typically, the bacterium is capable of producing these products from a gaseous substrate, such as syngas or an industrial waste gas.

公开(公告)号：US20170253896A1

公开(公告)日：2017-09-07

申请号：US15511684

申请日：2015-09-16

申请人： Global Bioenergies ,  Scientist of Fortune, S.A.

发明人： Philippe MARLIERE ,  Maria ANISSIMOVA ,  Mathieu ALLARD

IPC分类号： C12P7/52 ,  C12N15/52 ,  C12N9/12 ,  C12N9/88 ,  C12N9/10

CPC分类号： C12P7/52 ,  C12N9/1029 ,  C12N9/1217 ,  C12N9/88 ,  C12N15/52 ,  C12Y203/01008 ,  C12Y203/01019 ,  C12Y207/02001 ,  C12Y207/02007 ,  C12Y207/02014 ,  C12Y207/02015 ,  C12Y402/01017 ,  C12Y402/01018 ,  C12Y402/01055 ,  C12Y402/01059 ,  C12Y402/01074 ,  C12Y402/01116

摘要： Described is a method for the conversion of 3-methylcrotonyl-CoA into 3-hydroxy-3-methylbutyric acid comprising the steps of:(a) enzymatically converting 3-methylcrotonyl-CoA into 3-hydroxy-3-methylbutyryl-CoA; and (b) further enzymatically converting the thus produced 3-hydroxy-3-methylbutyryl-CoA into 3-hydroxy-3-methylbutyric acid wherein the enzymatic conversion of 3-hydroxy-3-methylbutyryl-CoA into 3-hydroxy-3-methylbutyric acid according to step (b) is achieved by first converting 3-hydroxy-3-methylbutyryl-CoA into 3-hydroxy-3-methylbutyryl phosphate and then subsequently converting the thus produced 3-hydroxy-3-methylbutyryl phosphate into 3-hydroxy-3-methylbutyric acid.

公开(公告)号：US20170137829A1

公开(公告)日：2017-05-18

申请号：US15348984

申请日：2016-11-11

申请人： Industrial Technology Research Institute

发明人： Pei-Ching Chang ,  Guang-Way Jang ,  Hsi-Yen Hsu ,  Hsiang-Yuan Chu ,  Jhong-De Lin ,  Ya-Lin Lin

IPC分类号： C12N15/63 ,  C12N9/10 ,  C12N9/04 ,  C12N9/12 ,  C12P7/56 ,  C12P7/46

CPC分类号： C12N15/63 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/1217 ,  C12N2510/02 ,  C12P7/46 ,  C12P7/56 ,  C12Y101/01001 ,  C12Y101/01027 ,  C12Y203/01008 ,  C12Y207/02001

摘要： A genetic engineered bacteria without or comprising a plurality of important metabolic enzyme related genes is provided. When the by-product or waste of fruit and vegetable is used as the culture medium, a large quantity of succinic acid or lactic acid can be produced via fermentation. A method of producing succinic acid and lactic acid using the genetic engineered bacteria is also provided.

公开(公告)号：US20180100168A1

公开(公告)日：2018-04-12

申请号：US15568347

申请日：2016-04-21

申请人： NUtech Ventures

发明人： Raghuveer Singh ,  Derrick White ,  Paul Blum

IPC分类号： C12P7/06 ,  C12N1/36 ,  C12N9/04 ,  C12N9/12 ,  C12N9/88 ,  C12P7/44 ,  C12P7/54 ,  C12P3/00 ,  C12R1/15 ,  C12R1/145 ,  C12R1/865

CPC分类号： C12P7/065 ,  C12N1/36 ,  C12N9/0006 ,  C12N9/1217 ,  C12N9/88 ,  C12P3/00 ,  C12P7/44 ,  C12P7/54 ,  C12R1/145 ,  C12R1/15 ,  C12R1/865 ,  C12Y101/01027 ,  C12Y207/02001 ,  C12Y401/0103 ,  C12Y401/01039 ,  Y02E50/17

摘要： This disclosure describes methods that allow for the uncoupling of microbial growth from product formation, which allows for maximal use of raw material and optimal end-product formation.

公开(公告)号：US09738875B2

公开(公告)日：2017-08-22

申请号：US15293191

申请日：2016-10-13

申请人： LanzaTech New Zealand Limited

发明人： Michael Koepke ,  Rasmus Overgaard Jensen ,  James Bruce Yarnton Haycock Behrendorff ,  Ryan Edward Hill

IPC分类号： C12N1/20 ,  C12N9/12 ,  C12N9/10 ,  C12N9/02 ,  C12P7/28 ,  C12P7/04 ,  C12P7/42 ,  C12P5/00

CPC分类号： C12P7/42 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/1217 ,  C12N9/16 ,  C12P5/007 ,  C12P5/026 ,  C12P7/04 ,  C12P7/18 ,  C12P7/24 ,  C12P7/28 ,  C12P7/625 ,  C12Y101/01001 ,  C12Y101/01002 ,  C12Y102/07005 ,  C12Y203/01008 ,  C12Y203/01009 ,  C12Y203/01019 ,  C12Y207/02001 ,  C12Y207/02007 ,  C12Y301/0202

摘要： The invention relates to a genetically engineered bacterium comprising an energy-generating fermentation pathway and methods related thereto. In particular, the invention provides a bacterium comprising a phosphate butyryltransferase (Ptb) and a butyrate kinase (Buk) (Ptb-Buk) that act on non-native substrates to produce a wide variety of products and intermediates. In certain embodiments, the invention relates to the introduction of Ptb-Buk into a C1-fixing microoorgansim capable of producing products from a gaseous substrate.

公开(公告)号：US20140315215A1

公开(公告)日：2014-10-23

申请号：US14344771

申请日：2012-09-14

申请人： John Mark Sutton ,  Philip James Alister Skipper

发明人： John Mark Sutton ,  Philip James Alister Skipper

IPC分类号： G01N33/573 ,  C07K16/44 ,  C12N9/12

CPC分类号： G01N33/573 ,  C07K16/44 ,  C07K2319/02 ,  C07K2319/30 ,  C12N9/12 ,  C12N9/1205 ,  C12N9/1217 ,  C12N9/1229 ,  C12Y207/0104 ,  C12Y207/02001 ,  C12Y207/04003

摘要： There is provided a single-chain fusion protein comprising: (i) a thermostable kinase and (ii) a single-domain antibody or single-domain antibody fragment. There is also provided a method of preparing a single-domain antibody or single-domain antibody fragment, the method comprising: (i) expressing the single-domain antibody or antibody fragment as a single-chain fusion protein with a thermostable kinase, in a host cell such as E. coli; and (ii) purifying the fusion protein from the cytoplasm of the host cell.

摘要翻译： 提供了单链融合蛋白，其包含：（i）热稳定激酶和（ii）单结构域抗体或单结构域抗体片段。 还提供了制备单结构域抗体或单域抗体片段的方法，所述方法包括：（i）将单结构域抗体或抗体片段表达为具有热稳定性激酶的单链融合蛋白， 宿主细胞如大肠杆菌; 和（ii）从宿主细胞的细胞质纯化融合蛋白。

公开(公告)号：US20120077239A9

公开(公告)日：2012-03-29

申请号：US12299070

申请日：2007-05-01

申请人： Arthur Josephus Shaw, IV ,  Sunil G. Desai ,  Lee R. Lynd ,  Mikhail V. Tyurin ,  Kara Podkaminer ,  John Bardsley ,  David Anthony Hogsett

发明人： Arthur Josephus Shaw, IV ,  Sunil G. Desai ,  Lee R. Lynd ,  Mikhail V. Tyurin ,  Kara Podkaminer ,  John Bardsley ,  David Anthony Hogsett

IPC分类号： C12P7/06 ,  C12N15/11 ,  C12N15/00 ,  C12N1/21

CPC分类号： C12N9/1029 ,  C12N9/0006 ,  C12N9/1217 ,  C12P7/10 ,  C12R1/145 ,  C12Y203/01008 ,  C12Y207/02001 ,  Y02E50/16 ,  Y02E50/17

摘要： Mutant thermophilic organisms that consume a variety of biomass derived substrates are disclosed herein. Strains of Thermoanaerobacterium saccharolyticum with acetate kinase and phosphotransacetylase expression eliminated are disclosed herein. Further, strain ALK1 has been engineered by site directed homologous recombination to knockout both acetic acid and lactic acid production. Continuous culture involving a substrate concentration challenge lead to evolution of ALK1, and formation of a more robust strain designated ALK2. The organisms may be utilized for example in thermophilic SSF and SSCF reactions performed at temperatures that are optimal for cellulase activity to produce near theoretical ethanol yields without expressing pyruvate decarboxylase.

摘要翻译： 本文公开了消耗多种生物质衍生底物的突变嗜热生物。 本文公开了消除了乙酸激酶和磷酸转乙酰酶表达的酿酒酵母属杆菌菌株。 此外，菌株ALK1已经通过位点定向同源重组进行工程化，以敲除乙酸和乳酸生产。 涉及底物浓度挑战的连续培养导致ALK1的进化，并形成称为ALK2的更强壮的菌株。 生物可以用于例如在对于纤维素酶活性最佳以在不表达丙酮酸脱羧酶的情况下产生接近理论乙醇产生的温度下进行的嗜热SSF和SSCF反应。

公开(公告)号：US20120034635A1

公开(公告)日：2012-02-09

申请号：US12954824

申请日：2010-11-26

申请人： Ching-Yi Hsu ,  Meng-Shun Huang ,  Hwan-You Chang ,  Hui-Ju Lee ,  Ming-Rong Ho

发明人： Ching-Yi Hsu ,  Meng-Shun Huang ,  Hwan-You Chang ,  Hui-Ju Lee ,  Ming-Rong Ho

IPC分类号： C12Q1/66 ,  C12P19/32

CPC分类号： C12P19/32 ,  C12Q1/04 ,  C12Q1/66 ,  C12Y113/12007 ,  C12Y207/02001 ,  C12Y207/04003 ,  C12Y207/07004

摘要： A method for amplifying adenosine triphosphate is provided, including mixing adenosine triphosphate sulfurylase, adenosine 5′ phosphosulfate, adenylate kinase, uridine triphosphate, acetate kinase, acetyl phosphate, luciferin and luciferase in the presence of ATP to form a mixture, and reacting the mixture to amplify ATP. A method and reagent for detecting a concentration of microorganisms are also provided.

摘要翻译： 提供了一种扩增三磷酸腺苷的方法，包括在ATP存在下混合三磷酸腺苷硫酸化酶，腺苷5'磷酸硫酸，腺苷酸激酶，尿苷三磷酸，乙酸激酶，乙酰磷酸，荧光素和萤光素酶，形成混合物， 放大ATP 还提供了用于检测微生物浓度的方法和试剂。