公开(公告)号：US20240200108A1

公开(公告)日：2024-06-20

申请号：US16500637

申请日：2018-04-05

申请人： POSTECH ACADEMY-INDUSTRY FOUNDATION ,  SEOUL NATIONAL UNIVERSITY R & DB FOUNDATION

发明人： Gyoo Yeol Jung ,  Sang Woo Seo ,  Chae Won Kang ,  Hyun Gyu Lim

IPC分类号： C12P7/46 ,  C12N15/70 ,  C12P5/02

CPC分类号： C12P7/46 ,  C12N15/70 ,  C12P5/026 ,  C12N2800/101 ,  C12N2830/36 ,  C12N2840/10

摘要： The present invention relates to a gene expression cassette including a synthetic 5′ untranslated region (5′ UTR), a promoter, and a regulatory gene; a recombinant vector including a replication origin and the gene expression cassette; a recombinant microorganism which has the recombinant vector introduced thereinto and shows alleviated segregational instability and; a method for preparing a recombinant microorganism having alleviated segregational instability by introducing the recombinant vector thereinto; and a method for quantitatively controlling a plasmid copy number in a recombinant microorganism. According to the present invention, removal of infA and efp, which are genes indispensable for cells, encoding respectively for a translation initiation factor and a protein elongation factor (EF-P), from a microbial chromosome and introduction of the gene expression cassette including the regulatory gene with Escherichia coli serving as a host allow the stable maintenance of plasmids in an antibiotic-free medium without causing intercellular intrinsic variations. In addition, the precise control of expression levels of infA and efp in the recombinant microorganism by means of a promoter can lead to the quantitative control of PCN at high yield as well. Therefore, the present invention can find a broad spectrum of applications in a variety of industries producing recombinant proteins.

公开(公告)号：US20230407362A1

公开(公告)日：2023-12-21

申请号：US18338958

申请日：2023-06-21

申请人： LanzaTech, Inc.

发明人： Sean Dennis Simpson ,  Michael Koepke ,  James MacAllister Clomburg ,  Juniper Dee Gramling ,  Stephanie Rhianon Jones ,  Jaeyoung Jung ,  Timothy James Politano

IPC分类号： C12P21/00 ,  C12P5/02 ,  C12P7/06 ,  C07K14/435

CPC分类号： C12P21/00 ,  C12P5/026 ,  C12P7/065 ,  C07K14/43586 ,  C07K14/43504 ,  C07K14/43568

摘要： Microorganisms are genetically engineered to continuously co-produce amino acids, proteins, microbial biomass, chemicals, or any combination thereof by microbial fermentation, particularly by microbial fermentation of a gaseous substrate. The microorganisms are C1-fixing. The production of ethylene, microbial biomass, and heterologous tandem repeat proteins can be improved. This can be effected by improved promoters or nutrient limiting means.

公开(公告)号：US20230407271A1

公开(公告)日：2023-12-21

申请号：US18339050

申请日：2023-06-21

申请人： LanzaTech, Inc.

发明人： Sean Dennis Simpson ,  Jennifer Rosa Holmgren ,  James MacAllister Clomburg ,  Jim Jeffrey Daleiden ,  Audrey Jean Harris ,  Stephanie Rhianon Jones ,  Michael Koepke ,  Timothy James Politano

IPC分类号： C12N9/02 ,  C12P5/02

CPC分类号： C12N9/0069 ,  C12Y113/12019 ,  C12P5/026

摘要： Methods and microorganisms are genetically engineered to continuously produce ethylene by microbial fermentation, particularly by the microbial fermentation of a gaseous substrate. The microorganisms are C1-fixing. Further, the gaseous substrate comprises CO2 and an energy source. The production of ethylene can be improved by varying promoters or nutrient limiting means.

公开(公告)号：US20230348886A1

公开(公告)日：2023-11-02

申请号：US18310636

申请日：2023-05-02

申请人： LanzaTech, Inc.

发明人： Ching Leang

IPC分类号： C12N9/88 ,  C12N9/10 ,  C12P7/6409 ,  C12P7/30 ,  C12P7/04 ,  C12P5/02 ,  C12P7/42 ,  C12P7/26 ,  C12P5/00 ,  C12P7/18

CPC分类号： C12N9/88 ,  C12Y401/01005 ,  C12N9/1029 ,  C12N9/13 ,  C12Y208/03 ,  C12Y401/01001 ,  C12P7/6409 ,  C12P7/30 ,  C12P7/04 ,  C12P5/026 ,  C12P7/42 ,  C12P7/26 ,  C12P5/007 ,  C12P7/18

摘要： Provided herein is a genetically engineered microorganism comprising knock-in of DNA at an acetolactate decarboxylase gene locus. Replacement of the acetolactate decarboxylase gene with DNA encoding one or more native or nonnative enzymes confers certain advantages, including fermentation stability and increased production of native and nonnative products from gaseous substrates.

公开(公告)号：US20230340543A1

公开(公告)日：2023-10-26

申请号：US17998759

申请日：2021-04-23

申请人： Iogen Corporation

发明人： Brian Foody ,  Jeffrey S. Tolan ,  Kristin Martens

IPC分类号： C12P7/06 ,  C10L1/02 ,  C12P5/02 ,  C12P19/14 ,  C12P19/02 ,  C08H8/00

CPC分类号： C12P7/06 ,  C08H8/00 ,  C10L1/02 ,  C12P5/026 ,  C12P19/02 ,  C12P19/14 ,  C10L2200/0469 ,  C10L2290/10 ,  C10L2290/12 ,  C10L2290/26 ,  C12P2201/00

摘要： A process for converting lignocellulosic biomass to fuel, wherein lignocellulosic feedstock is soaked in a sulfuric acid solution to demineralize the feedstock, the demineralized feedstock is pretreated at a temperature between 150° C. and 230° C. and a pH between 1 and 2.5, at least part of the pretreated material is converted to a fermentation production such as ethanol, and at least a portion of a secondary stream, such as still bottoms from the distillation of ethanol, is converted to biogas by anaerobic digestion. Soaking the lignocellulosic feedstock in sulfuric acid solution reduces the amount of sulfuric acid required for the pretreatment, and thus the amount of sulfate carried downstream to the anaerobic digestion. This increases the biogas yield and/or xylose yield. A recycling process, wherein mineralized soaking liquid produced in the soaking process is fed to cation exchange to remove minerals, reduces excess waste of the sulfuric acid and water usage.

公开(公告)号：US20230183627A1

公开(公告)日：2023-06-15

申请号：US17912242

申请日：2021-03-19

申请人： CEMVITA FACTORY, INC.

发明人： Tahereh KARIMI ,  Truong Huu NGUYEN ,  MohammadMatin HANIFZADEH ,  Samantha COOK ALBRIGHT ,  Miguel Eugenio CUEVA

IPC分类号： C12M1/00 ,  C12M1/107 ,  C12P21/00 ,  C12P5/02

CPC分类号： C12M23/58 ,  C12M21/04 ,  C12P21/00 ,  C12P5/026

摘要： The present disclosure relates to biomanufacturing systems for producing an organic product. The present disclosure relates to recombinant microorganisms having an improved organic substrate producing ability, and to recombinant microorganisms having an improved organic product producing ability. A benefit of the systems and recombinant microorganisms disclosed herein can include an ability to separately produce an organic product and an organic substrate that generates a culture impurity during its production. The present disclosure relates to methods of producing an organic product using biomanufacturing systems and recombinant microorganisms disclosed herein.

公开(公告)号：US20190194695A1

公开(公告)日：2019-06-27

申请号：US16184377

申请日：2018-11-08

申请人： Arizona Board of Regents on Behalf of Arizona State University

发明人： David Nielsen ,  Shawn Pugh ,  Rebekah McKenna

IPC分类号： C12P5/02 ,  C12N9/88

CPC分类号： C12P5/026 ,  C12N9/88 ,  C12Y401/01 ,  Y02E50/343

摘要： A method for the in vivo production of 1,3-butadiene from 2,4-pentadienoate is described (FIG. 1). Enzymes capable of decarboxylating 2,4-pentadienoate to 1,3-butadiene have been discovered. Recombinant expression of these newly discovered enzymes has resulted in the engineering of microorganisms capable of producing 1,3-butadiene when cultured in the presence of 2,4-pentadienoate. 1,3-butadienoate is an important monomer used in the manufacturing of rubbers and plastics. This invention will help to enable the biological production of 1,3-butadiene from renewable resources such as sugar, for example.

公开(公告)号：US20180327790A1

公开(公告)日：2018-11-15

申请号：US15763257

申请日：2016-09-23

申请人： DANMARKS TEKNISKE UNIVERSITET

发明人： Peter RUGBJERG ,  Kira SARUP-LYTZEN ,  Morten SOMMER

IPC分类号： C12P7/42 ,  C12N15/72 ,  C12N9/16

CPC分类号： C12P7/42 ,  C12N9/16 ,  C12N15/1086 ,  C12N15/67 ,  C12N15/72 ,  C12P1/02 ,  C12P1/04 ,  C12P5/026 ,  C12P7/40 ,  C12P7/64 ,  C12P21/02

摘要： The invention provides a genetically modified micro-organism for intracellular biosynthesis of a cellular metabolite, comprising a synthetic error correction system having a penalty gene, whose expression leads to arrested growth or cell death (e.g. a toxin gene) in combination with a survival gene, whose expression provides an antidote that restores cell viability and normal growth (e.g. a cognate antitoxin gene). Alternatively, the system has a survival gene, alone, whose expression is essential for growth (i.e. essential gene). The synthetic error correction system further comprises a biosensor, whose function is to induce expression of the survival gene which leads to cell growth, only, when the cell produces a pre-defined level of a given metabolite. The invention further encompasses: a method for producing the genetically modified micro-organism; a method for producing a cellular metabolite with the genetically modified micro-organism; and use of the genetically modified micro-organism for producing a cellular metabolite.

公开(公告)号：US10059963B2

公开(公告)日：2018-08-28

申请号：US14885311

申请日：2015-10-16

申请人： Braskem S.A.

发明人： Mateus Schreiner Garcez Lopes ,  Avram Michael Slovic ,  Iuri Estrada Gouvea ,  Johana Rincones Perez ,  Lucas Pedersen Parizzi

IPC分类号： C12P5/02 ,  C12N15/52 ,  C12N9/88

CPC分类号： C12P5/026 ,  C12N9/88 ,  C12N15/52 ,  C12P5/02 ,  C12Y402/01127

摘要： The present disclosure generally relates to microorganisms that comprise one or more polynucleotides coding for enzymes in one or more pathways that catalyze a conversion of a fermentable carbon source to butadiene. Also provided are methods of using the microorganisms in industrial processes including, for use in the production of butadiene and products derived therefrom.

公开(公告)号：US20180187121A1

公开(公告)日：2018-07-05

申请号：US15736581

申请日：2016-06-14

申请人： White Dog Labs, Inc.

发明人： Aharon M. EYAL ,  Bryan P. TRACY ,  Christopher Joseph MCWILLIAMS

IPC分类号： C11B1/10 ,  C07C7/10 ,  C12P7/64 ,  C12P5/02

CPC分类号： C11B1/104 ,  C07C7/10 ,  C07C11/18 ,  C11B1/10 ,  C11B1/102 ,  C11B3/00 ,  C11B3/001 ,  C12P5/026 ,  C12P7/6409 ,  Y02E50/343 ,  Y02P30/20

摘要： Disclosed are methods for making one or more bioproducts such as isoprene derivatives, fatty acids, fatty acid derivatives, fatty alcohols, fatty alcohol derivatives, mixtures thereof, and compositions having one or more such bioproducts with microorganisms and for separating the bioproduct(s) or bioproduct composition(s). The methods include providing an extractant having a solvent with a boiling point at atmospheric pressure of less than 20° C.; feeding an aqueous suspension of cells that have produced the bioproduct(s) into a pressurized column; feeding the extractant into the pressurized column; removing an extract having the extractant and the bioproduct(s) from said pressurized column; and separating the extractant from the removed extract to obtain a separated oil-soluble composition having the bioproduct.