公开(公告)号：US20190194695A1

公开(公告)日：2019-06-27

申请号：US16184377

申请日：2018-11-08

Applicant: Arizona Board of Regents on Behalf of Arizona State University

Inventor： David Nielsen ,  Shawn Pugh ,  Rebekah McKenna

IPC: C12P5/02 ,  C12N9/88

CPC classification number: C12P5/026 ,  C12N9/88 ,  C12Y401/01 ,  Y02E50/343

Abstract: A method for the in vivo production of 1,3-butadiene from 2,4-pentadienoate is described (FIG. 1). Enzymes capable of decarboxylating 2,4-pentadienoate to 1,3-butadiene have been discovered. Recombinant expression of these newly discovered enzymes has resulted in the engineering of microorganisms capable of producing 1,3-butadiene when cultured in the presence of 2,4-pentadienoate. 1,3-butadienoate is an important monomer used in the manufacturing of rubbers and plastics. This invention will help to enable the biological production of 1,3-butadiene from renewable resources such as sugar, for example.