公开(公告)号：US12098407B2

公开(公告)日：2024-09-24

申请号：US17616843

申请日：2020-06-05

Applicant: AMYRIS, INC.

Inventor： Penelope R. Chua ,  Joshua Adam Lerman ,  Thomas Jon Scherbart ,  Chandresh Thakker ,  Annie Ening Tsong ,  Hanxiao Jiang

IPC: C12N1/14 ,  C12N1/18 ,  C12N9/02 ,  C12N9/14 ,  C12N15/81 ,  C12P5/00

CPC classification number: C12P5/007 ,  C12N1/18 ,  C12N9/0036 ,  C12N9/14 ,  C12N15/81

Abstract: Provided herein are compositions and methods for uncoupling the yield and productivity of an isoprenoid compound produced in a host cell. In some embodiments, the yield and productivity are uncoupled by genetically modifying the host cell to reduce flux through the citric acid cycle (TCA). In other embodiments, the yield and productivity are uncoupled by reducing the levels of ATP in the host cell.

公开(公告)号：US20240287560A1

公开(公告)日：2024-08-29

申请号：US17757752

申请日：2020-12-22

Applicant: FIRMENICH SA

Inventor： Laurent DAVIET ,  Yang HUANG ,  Vincent HARRACA ,  Michel SCHALK ,  Monica BANDERA

IPC: C12P15/00 ,  A01N65/08 ,  A01P7/04 ,  A61K31/045 ,  A61K31/11 ,  A61K36/185 ,  C11B9/00 ,  C11B9/02 ,  C12N9/02 ,  C12N9/88 ,  C12P5/00 ,  C12P7/02

CPC classification number: C12P15/00 ,  A01N65/08 ,  A01P7/04 ,  A61K31/045 ,  A61K31/11 ,  A61K36/185 ,  C11B9/0003 ,  C11B9/02 ,  C12N9/0042 ,  C12N9/0071 ,  C12N9/88 ,  C12P5/007 ,  C12P7/02 ,  C12Y106/02004 ,  C12Y402/03082

Abstract: Described herein is a biochemically produced sandalwood oil including at least 85% santalol and bergamotol and 1% or less cis-lanceol, where the oil has one or more of the following features: i) 5% or less alpha-santalal; ii) 5% or less farnesol; and iii) 0.5% or less spirosantalol. Also described herein are a perfuming composition including the sandalwood oil and a perfuming consumer product including the sandalwood oil. Also described herein are a method of using the biochemically produced sandalwood oil as an anti-microbial and anti-inflammatory agent, and an arthropod control composition including the sandalwood oil.

公开(公告)号：US20240060075A1

公开(公告)日：2024-02-22

申请号：US18454635

申请日：2023-08-23

Applicant: AMYRIS, INC.

Inventor： Andrew MAIN ,  Grzegorz WOJCIECHOWSKI ,  Yue YANG ,  Lishan ZHAO

IPC: C12N15/52 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12P5/00

CPC classification number: C12N15/52 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12P5/007 ,  C12Y205/01029 ,  C12Y402/03015

Abstract: Provided herein are compositions and methods for producing myrcene by culturing genetically modified microbial host cells that express a myrcene synthase and optionally a geranyl pyroplosphate synthase. Also provided herein are isolated nucleic acid molecules that encode myrcene synthase variants derived from the Ocimum species myrcene synthase, which comprise one or more amino acid substitutions that improve in vivo performance of myrcene synthase in genetically modified microbial host cells. Also provided herein are isolated myrcene synthase variants that exhibit an improved activity for converting geranyl diphosphate into myrcene.

公开(公告)号：US11845940B2

公开(公告)日：2023-12-19

申请号：US17187697

申请日：2021-02-26

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  NATIONAL TECHNOLOGY AND ENGINEERING SOLUTIONS OF SANDIA, LLC

Inventor： Gina Marie Geiselman ,  James Kirby ,  Taek Soon Lee ,  John M. Gladden ,  Blake A. Simmons

IPC: C12N15/81 ,  C12N9/88 ,  C12P5/00 ,  C12N1/16 ,  C10L1/04

CPC classification number: C12N15/815 ,  C10L1/04 ,  C12N1/16 ,  C12N9/88 ,  C12P5/007 ,  C12Y402/03037 ,  C10L2200/043 ,  C10L2270/04 ,  C10L2290/26

Abstract: The present invention provides for a genetically modified fungal host cell capable of producing prespatane and/or epi-isozizaene comprising prespatane synthase (PPS) and/or epi-isozizaene synthase (EIZS).

公开(公告)号：US20230323314A1

公开(公告)日：2023-10-12

申请号：US18161938

申请日：2023-01-31

Applicant: MANUS BIO INC.

Inventor： Jason DONALD ,  Christopher PIRIE ,  Liwei LI ,  Huey-Ming MAK ,  Srishti TIBREWALA ,  Ajikumar PARAYIL KUMARAN

IPC: C12N15/52 ,  C12N9/00 ,  C12N9/02 ,  C12P19/56 ,  C12P5/00 ,  C12N15/70

CPC classification number: C12N9/0079 ,  C12N9/00 ,  C12N15/52 ,  C12N15/70 ,  C12P5/007 ,  C12P19/56 ,  C07K2319/03

Abstract: The present invention relates to the production of chemical species in bacterial host cells. Particularly, the present invention provides for the production of chemical species in Escherichia coli (E. coli) host cells that functionally express engineered P450 enzymes.

公开(公告)号：US20230167457A1

公开(公告)日：2023-06-01

申请号：US17787493

申请日：2020-12-20

Applicant: ENGENES BIOTECH GMBH

Inventor： Juergen Mairhofer

IPC: C12N15/70 ,  C12M1/06 ,  C12M1/00 ,  C12P5/00

CPC classification number: C12N15/70 ,  C12M27/02 ,  C12M23/58 ,  C12P5/007

Abstract: The present invention is in the field of recombinant biotechnology, in particular in the field of protein expression and nucleotide production. The invention generally relates to systems and processes that are suitable or comprise a two stage production process, in which the growth of the bacterial host cell is spatiotemporally separated from the production of the protein or nucleic acid of interest. Accordingly, the present invention relates to a system and a process for use in continuous production of a protein of interest or a nucleotide of interest by a bacterial host cell.

公开(公告)号：US11660961B2

公开(公告)日：2023-05-30

申请号：US17067610

申请日：2020-10-09

Applicant: The Regents of the University of California

Inventor： Taek Soon Lee ,  Aram Kang

IPC: C12N9/88 ,  B60K35/00 ,  C07F9/09 ,  C12P5/00 ,  C12N9/16 ,  C12P7/04 ,  C12N9/04 ,  C12N9/10 ,  C12N9/12 ,  C08F136/08 ,  B65G69/00 ,  G01S13/931

CPC classification number: B60K35/00 ,  B65G69/006 ,  C07F9/091 ,  C07F9/098 ,  C08F136/08 ,  C12N9/0006 ,  C12N9/1025 ,  C12N9/1205 ,  C12N9/16 ,  C12N9/88 ,  C12P5/007 ,  C12P7/04 ,  C12Y101/01088 ,  C12Y203/0301 ,  C12Y207/01036 ,  C12Y401/01033 ,  B60K2370/173 ,  G01S2013/9314 ,  G01S2013/9317 ,  G01S2013/9323 ,  G01S2013/9324

Abstract: The present invention provides for a genetically modified host cell capable of producing isopentenol and/or 3-methyl-3-butenol, comprising (a) an increased expression of phosphomevalonate decarboxylase (PMD) (b) an increased expression of a phosphatase capable of converting isopentenol into 3-methyl-3-butenol, (c) optionally the genetically modified host cell does not express, or has a decreased expression of one or more of NudB, phosphomevalonate kinase (PMK), and/or PMD, and (d) optionally one or more further enzymes capable of converting isopentenol and/or 3-methyl-3-butenol into a third compound, such as isoprene.

公开(公告)号：US20190211364A1

公开(公告)日：2019-07-11

申请号：US16310910

申请日：2017-06-30

Applicant: INTERNATIONAL FLAVORS & FRAGRANCES INC.

Inventor： Ingo De Abreu ,  Mauricio Poulsen

IPC: C12P5/00 ,  C12N9/88 ,  C12N9/02

CPC classification number: C12P5/007 ,  C12N9/0042 ,  C12N9/88 ,  C12N15/52 ,  C12Y106/02004

Abstract: Compositions and methods for synthesizing a citrus terpenoid using a recombinant host cell that produces isopentenyl pyrophosphate and dimethylallyl pyrophosphate, and expresses one or more enzymes that convert the IPP and DMAPP to a citrus terpenoid are described.

公开(公告)号：US20180327794A1

公开(公告)日：2018-11-15

申请号：US15775139

申请日：2016-11-21

Applicant: Novozymes A/S

Inventor： Suchitra Tripathy

IPC: C12P17/02 ,  C08C19/06

CPC classification number: C12P17/02 ,  C08C19/06 ,  C08L15/00 ,  C12N9/0006 ,  C12N9/0061 ,  C12N9/0065 ,  C12P5/007 ,  C12P7/00

Abstract: Disclosed is a method for epoxidating natural rubber using enzymes that generate reactive oxygen species.

公开(公告)号：US20180282766A1

公开(公告)日：2018-10-04

申请号：US15924700

申请日：2018-03-19

Applicant: Massachusetts Institute of Technology ,  National University of Singapore

Inventor： Parayil K. Ajikumar ,  Gregory Stephanopoulos ,  Heng Phon Too

IPC: C12P5/00 ,  C12P19/56 ,  C12P23/00 ,  C12P17/02 ,  C12P15/00 ,  C12P9/00 ,  C12N15/70 ,  C12P7/42 ,  C12Q1/689

CPC classification number: C12P5/007 ,  C12N15/70 ,  C12P7/42 ,  C12P9/00 ,  C12P15/00 ,  C12P17/02 ,  C12P19/56 ,  C12P23/00 ,  C12Q1/689 ,  C12Q2600/158 ,  H05K999/99

Abstract: The invention relates to the production of one or more terpenoids through microbial engineering, and relates to the manufacture of products comprising terpenoids.