公开(公告)号：US20240363243A1

公开(公告)日：2024-10-31

申请号：US18424596

申请日：2024-01-26

申请人： Tata Consultancy Services Limited

发明人： CHANDRANI BOSE ,  MOHAMMED MONZOORUL HAQUE ,  RASHMI SINGH ,  SHARMILA SHEKHAR MANDE ,  VENKATA SIVA KUMAR REDDY CHENNAREDDY

IPC分类号： G16H50/20 ,  A61B10/00 ,  C12Q1/689 ,  G16B40/00

CPC分类号： G16H50/20 ,  A61B10/0041 ,  C12Q1/689 ,  G16B40/00 ,  C12Q2600/16

摘要： The present disclosure is related to methods and systems for predicting a category of mammographic breast density (MBD) for a subject using a microbial profile obtained from biological sample of the subject. The state-of-art diagnostic/screening strategies for breast cancer are limited by one or more of factors like technical shortcomings, radiation exposure, and physical discomfort. In the present disclosure, a biological sample is collected from a subject. Then a quantitative abundance of each of a plurality of predetermined microbes associated with the biological sample is determined using a set of probes through a multiplex quantitative Polymerase Chain Reaction (qPCR) technique. Further the quantitative abundance is collated to obtain a microbial abundance matrix. Next a model score is determined based on the microbial abundance matrix, using a pre-determined machine learning (ML) model. Lastly the risk category of breast cancer of the subject is assessed based on the model score.

公开(公告)号：US20240360522A1

公开(公告)日：2024-10-31

申请号：US18287776

申请日：2022-04-21

申请人： Rutgers, The State University of New Jersey

发明人： Bassel Ghaddar ,  Subhajyoti De

IPC分类号： C12Q1/689 ,  C12Q1/6886 ,  C12Q1/6895

CPC分类号： C12Q1/689 ,  C12Q1/6886 ,  C12Q1/6895 ,  C12Q2600/118 ,  C12Q2600/158

摘要： Disclosed herein are systems and methods for identifying biomarkers. Biomarker identification can be achieved while increasing efficiency and decreasing data and computation complexity but maintaining accuracy. Such biomarker identification can be achieved via analysis of differential gene expression, such as determined using single cell RNA-sequencing data sets.

公开(公告)号：US20240352540A1

公开(公告)日：2024-10-24

申请号：US18642242

申请日：2024-04-22

申请人： McMaster University

发明人： Yingfu Li ,  Shuwen Qian ,  Dingran Chang ,  Jimmy Gu ,  Bruno Salena

IPC分类号： C12Q1/689 ,  C12Q1/6818

CPC分类号： C12Q1/689 ,  C12Q1/6818

摘要： This disclosure relates to catalytic nucleic acids, catalytic nucleic acid probes, biosensors, and kits for detecting the presence of Clostridium difficile. Also provided are methods for detecting the presence of Clostridium difficile in a test sample, using the catalytic nucleic acids, catalytic nucleic acid probes, biosensors, and kits.

公开(公告)号：US12123062B2

公开(公告)日：2024-10-22

申请号：US18054778

申请日：2022-11-11

申请人： The Florida State University Research Foundation, Inc. ,  The United State of America, as presented by the Secretary of Agriculture

发明人： Prashant Singh ,  Joseph M. Bosilevac

IPC分类号： C12Q1/68 ,  C12Q1/689 ,  C12R1/42

CPC分类号： C12Q1/689 ,  C12Q2600/16 ,  C12R2001/42

摘要： Disclosed herein are methods and kits for quantifying the presence of a microorganism in a sample. Specifically, disclosed are methods for quantifying a pathogen in a sample, such as a food sample, to determine if the levels of pathogen present in the sample are within an acceptable range.

公开(公告)号：US20240309466A1

公开(公告)日：2024-09-19

申请号：US18572850

申请日：2022-06-21

申请人： The Johns Hopkins University

发明人： Subhra Chakraborty

IPC分类号： C12Q1/689 ,  C12Q1/6844

CPC分类号： C12Q1/689 ,  C12Q1/6844

摘要： Disclosed herein are methods for detecting a pathogenic microorganism in a sample. The methods can include the steps of contacting the sample with a lysis solution to form a mixture; filtering the mixture through a filter to form a filtered mixture, wherein the filtered mixture comprises DNA or RNA of the target microorganism; contacting the filtered mixture with loop mediated isothermal amplification (LAMP) reagents and one or more primer sets specific to the DNA or RNA of the target microorganism, amplifying the DNA or RNA of the target microorganism, thereby producing one or more amplicons; and detecting the presence or absence of the one or more amplicons, wherein the presence of the one or more of the amplicons indicates the presence of the pathogenic microorganism. The LAMP reagents and the one or more primer sets can be lyophilized.

公开(公告)号：US12084725B2

公开(公告)日：2024-09-10

申请号：US17147549

申请日：2021-01-13

申请人： BENED BIOMEDICAL CO., LTD.

发明人： Ying-Chieh Tsai ,  Chien-Chen Wu ,  Chih-Chieh Hsu

IPC分类号： C12Q1/68 ,  C12Q1/6851 ,  C12Q1/689

CPC分类号： C12Q1/689 ,  C12Q1/6851

摘要： Provided are compositions and methods for identifying a specific strain of Lactobacillus plantarum, e.g., Lactobacillus plantarum subsp. plantarum PS128 deposited under DSMZ Accession No. DSM 28632.

公开(公告)号：US20240294995A1

公开(公告)日：2024-09-05

申请号：US18593638

申请日：2024-03-01

申请人： Hangzhou Medical College

发明人： Fangwei Dai ,  Huiqiong Yan ,  Wei Han ,  Shasang Zhou ,  Jiangtao Du ,  Junhao Tao ,  Honggang Guo ,  Huazhong Ying

IPC分类号： C12Q1/689 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6844

CPC分类号： C12Q1/689 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6844

摘要： The present disclosure provides a primer combination, a kit, and a method for detecting Clostridium piliforme based on loop-mediated isothermal amplification-lateral flow dipstick (LAMP-LFD), belonging to the technical field of medical molecular biology detection. In the present disclosure, the primer combination includes a forward outer primer F3, a backward outer primer B3, a forward inner primer FIP, a backward inner primer BIP, a loop primer LF, and a probe PB1. The present disclosure further provides a kit and a method for detecting Clostridium piliforme based on the primer combination. The kit and the method can detect the Clostridium piliforme with a simple detection process, a low time consumption, easy determination of results, and high detection accuracy and sensitivity.

公开(公告)号：US12077827B2

公开(公告)日：2024-09-03

申请号：US16651426

申请日：2018-09-28

申请人： INSERM (INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE) ,  UNIVERSITÉ DE BRETAGNE OCCIDENTALE ,  ETABLISSEMENT FRANCAIS DU SANG (EFS) ,  CENTRE HOSPITALIER REGIONSL ET UNIVERSITAIRE DE BREST ,  INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT

发明人： Geneviève Hery-Arnaud ,  Jérôme Mounier ,  Marlène Keravec ,  Stanislas Mondot ,  Patricia Lepage ,  Charles-Antoine Guilloux

IPC分类号： C12Q1/68 ,  A61K35/741 ,  C07K16/12 ,  C12Q1/689 ,  A61K35/00 ,  A61K45/06

CPC分类号： C12Q1/689 ,  A61K35/741 ,  C07K16/1214 ,  A61K2035/115 ,  A61K45/06 ,  C12Q2600/118 ,  G01N2800/12 ,  G01N2800/50

摘要： The present invention relates to methods for predicting the risk of developing pulmonary colonization/infection by P. aeruginosa. The inventors analyzed the respiratory tract microbiota from 65 patients sputum samples and compared microbiota data. The inventors found that patients that will remain uninfected from P. aeruginosa exhibited 3-fold higher abundance of Porphyromonas compared to the other groups. In particular, the present invention relates to a method for predicting the risk of developing pulmonary colonization/infection by P. aeruginosa in a subject suffering from cystic fibrosis (CF) comprising measuring the abundance of Porphyromonas genus bacteria in a biological sample obtained from said subject.

公开(公告)号：US20240287607A1

公开(公告)日：2024-08-29

申请号：US18410314

申请日：2024-01-11

申请人： IMMUNEXPRESS PTY LTD

发明人： Richard Bruce Brandon ,  Leo Charles McHugh

IPC分类号： C12Q1/6883 ,  C12Q1/689 ,  G16B25/00 ,  G16B25/10

CPC分类号： C12Q1/6883 ,  C12Q1/689 ,  G16B25/00 ,  G16B25/10 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/158

摘要： Disclosed are method and apparatus for identifying biomarkers and in particular for identifying biomarkers for use in making clinical assessments, such as early diagnostic, diagnostic, disease stage, disease severity, disease subtype, response to therapy or prognostic assessments. In one particular example, the techniques are applied to allow assessments of patients suffering from, suspected of suffering from, or with clinical signs of SIRS (Systemic Inflammatory Response Syndrome) being either infection-negative SIRS or infection-positive SIRS.

公开(公告)号：US12071673B2

公开(公告)日：2024-08-27

申请号：US16694845

申请日：2019-11-25

申请人： Cedars-Sinai Medical Center

发明人： Mark Pimentel ,  Ruchi Mathur ,  Christopher Chang

IPC分类号： C12Q1/689 ,  A61K31/137 ,  A61K31/155 ,  A61K31/22 ,  A61K31/337 ,  A61K31/351 ,  A61K31/357 ,  A61K31/366 ,  A61K31/40 ,  A61K31/404 ,  A61K31/4164 ,  A61K31/437 ,  A61K31/454 ,  A61K31/47 ,  A61K31/505 ,  A61K31/55 ,  A61K31/7036 ,  A61K31/7048 ,  A61K35/741 ,  A61K45/06

CPC分类号： C12Q1/689 ,  A61K31/137 ,  A61K31/155 ,  A61K31/22 ,  A61K31/337 ,  A61K31/351 ,  A61K31/357 ,  A61K31/366 ,  A61K31/40 ,  A61K31/404 ,  A61K31/4164 ,  A61K31/437 ,  A61K31/454 ,  A61K31/47 ,  A61K31/505 ,  A61K31/55 ,  A61K31/7036 ,  A61K31/7048 ,  A61K35/741 ,  A61K45/06 ,  C12Q2600/106 ,  A61K35/741 ,  A61K2300/00 ,  A61K31/155 ,  A61K2300/00 ,  A61K31/437 ,  A61K2300/00 ,  A61K31/7036 ,  A61K2300/00 ,  A61K31/4164 ,  A61K2300/00 ,  A61K31/40 ,  A61K2300/00 ,  A61K31/404 ,  A61K2300/00 ,  A61K31/366 ,  A61K2300/00 ,  A61K31/47 ,  A61K2300/00 ,  A61K31/22 ,  A61K2300/00 ,  A61K31/505 ,  A61K2300/00 ,  A61K31/137 ,  A61K2300/00 ,  A61K31/7048 ,  A61K2300/00 ,  A61K31/454 ,  A61K2300/00

摘要： The invention described herein provides for methods and systems for determining, selecting, and/or treating diseases and conditions caused by or associated with high quantities of methanogens in a subject, or diseases and conditions caused by or associated with low quantities of methanogens in a subject. In various embodiments, a therapy to inhibit the growth of methanogens or to promote the growth of methanogens are selected and/or administered to a subject in need thereof.