公开(公告)号：US20240294995A1

公开(公告)日：2024-09-05

申请号：US18593638

申请日：2024-03-01

申请人： Hangzhou Medical College

发明人： Fangwei Dai ,  Huiqiong Yan ,  Wei Han ,  Shasang Zhou ,  Jiangtao Du ,  Junhao Tao ,  Honggang Guo ,  Huazhong Ying

IPC分类号： C12Q1/689 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6844

CPC分类号： C12Q1/689 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6844

摘要： The present disclosure provides a primer combination, a kit, and a method for detecting Clostridium piliforme based on loop-mediated isothermal amplification-lateral flow dipstick (LAMP-LFD), belonging to the technical field of medical molecular biology detection. In the present disclosure, the primer combination includes a forward outer primer F3, a backward outer primer B3, a forward inner primer FIP, a backward inner primer BIP, a loop primer LF, and a probe PB1. The present disclosure further provides a kit and a method for detecting Clostridium piliforme based on the primer combination. The kit and the method can detect the Clostridium piliforme with a simple detection process, a low time consumption, easy determination of results, and high detection accuracy and sensitivity.