公开(公告)号：US12122995B2

公开(公告)日：2024-10-22

申请号：US16726163

申请日：2019-12-23

申请人： DNA2.0, INC.

发明人： Jeremy Minshull ,  Mark Welch ,  Sridhar Govindrajan ,  Maggie Lee ,  Kate Caves ,  Jon Ness

IPC分类号： C12N9/12 ,  C12N1/16 ,  C12N1/18 ,  C12N15/10 ,  C12N15/52 ,  C12N15/62 ,  C12N15/63 ,  C12N15/67 ,  C12N15/81 ,  C12N15/85 ,  C12N15/90 ,  C12R1/84 ,  C12R1/865

CPC分类号： C12N1/165 ,  C12N1/185 ,  C12N9/12 ,  C12N9/1241 ,  C12N15/1082 ,  C12N15/52 ,  C12N15/625 ,  C12N15/63 ,  C12N15/81 ,  C12N15/815 ,  C12N15/85 ,  C12N15/8509 ,  C12N15/90 ,  C12Y207/00 ,  C12Y207/07 ,  C07K2319/09 ,  C12N15/67 ,  C12N2800/90 ,  C12N2830/007 ,  C12N2830/40 ,  C12N2830/42 ,  C12N2840/203 ,  C12R2001/84 ,  C12R2001/865

摘要： The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.

公开(公告)号：US12098407B2

公开(公告)日：2024-09-24

申请号：US17616843

申请日：2020-06-05

申请人： AMYRIS, INC.

发明人： Penelope R. Chua ,  Joshua Adam Lerman ,  Thomas Jon Scherbart ,  Chandresh Thakker ,  Annie Ening Tsong ,  Hanxiao Jiang

IPC分类号： C12N1/14 ,  C12N1/18 ,  C12N9/02 ,  C12N9/14 ,  C12N15/81 ,  C12P5/00

CPC分类号： C12P5/007 ,  C12N1/18 ,  C12N9/0036 ,  C12N9/14 ,  C12N15/81

摘要： Provided herein are compositions and methods for uncoupling the yield and productivity of an isoprenoid compound produced in a host cell. In some embodiments, the yield and productivity are uncoupled by genetically modifying the host cell to reduce flux through the citric acid cycle (TCA). In other embodiments, the yield and productivity are uncoupled by reducing the levels of ATP in the host cell.

公开(公告)号：US20240309406A1

公开(公告)日：2024-09-19

申请号：US18257456

申请日：2021-12-14

申请人： SPECIALTY OPERATIONS FRANCE

发明人： Valéry Dambrin ,  Mirjana Gelo-Pujic

IPC分类号： C12P7/26 ,  C12N1/18 ,  C12N9/02

CPC分类号： C12P7/26 ,  C12N1/185 ,  C12N9/001 ,  C12N2511/00

摘要： The present invention relates to a process for preparing natural raspberry ketone, comprising a step (a) of bioconversion of p-coumaric acid allowing the preparation of p-hydroxybenzaldehyde, a step (b) in which the p-hydroxybenzaldehyde obtained on conclusion of step (a) is condensed with acetone to allow the formation of p-hydroxybenzalacetone, a step (c) in which the p-hydroxybenzalacetone obtained on conclusion of step (b) is converted into raspberry ketone by bioconversion or biocatalysis.

公开(公告)号：US20240309313A1

公开(公告)日：2024-09-19

申请号：US18577903

申请日：2022-07-07

申请人： VIB VZW ,  Katholieke Universiteit Leuven ,  THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY

发明人： Kevin Verstrepen ,  Ping-Wei Ho ,  Dan Jarosz

IPC分类号： C12N1/18 ,  A61K36/064 ,  C12N9/22 ,  C12N15/11 ,  C12N15/81 ,  C12P7/16 ,  C12P7/54 ,  C12R1/865

CPC分类号： C12N1/185 ,  A61K36/064 ,  C12N9/22 ,  C12N15/111 ,  C12N15/81 ,  C12P7/16 ,  C12P7/54 ,  C12N2310/20 ,  C12R2001/865

摘要： The present invention relates to the field of yeast fermentations. More particularly, the invention relates to mutant alleles useful to engineer industrially relevant traits in yeast.

公开(公告)号：US20240301434A1

公开(公告)日：2024-09-12

申请号：US18658840

申请日：2024-05-08

申请人： Wisconsin Alumni Research Foundation

发明人： Chris Todd Hittinger ,  David Peris Navarro ,  EmilyClare Patricia Baker

IPC分类号： C12N15/81 ,  C12N1/14 ,  C12N1/18 ,  C12R1/645

CPC分类号： C12N15/81 ,  C12N1/145 ,  C12N1/18 ,  C12R2001/645

摘要： Herein we demonstrate that mitochondrial DNA (mtDNA) influences temperature tolerance in Saccharomyces yeasts. The present invention provides methods for manipulating the mitotype of yeast, including methods to produce synthetic yeast hybrids with a selected mitotype and methods to exchange the native mtDNA present in polyploid yeast with mtDNA from a desired source. Saccharomyces cerevisiae x Saccharomyces eubayanus hybrids with selected mitotypes are also provided. The yeast and methods of the present invention may be utilized in a variety of applications, including in fermentation to produce beer and wine.

公开(公告)号：US20240200021A1

公开(公告)日：2024-06-20

申请号：US18555663

申请日：2022-04-15

申请人： INBIOSE N.V.

发明人： Joeri Beauprez ,  Pieter Coussement ,  Thomas Decoene

IPC分类号： C12N1/20 ,  C12N1/18 ,  C12N9/10 ,  C12P9/00 ,  C12P19/04 ,  C12P19/12 ,  C12P19/26 ,  C12R1/19

CPC分类号： C12N1/205 ,  C12N1/185 ,  C12N9/1081 ,  C12P9/00 ,  C12P19/04 ,  C12P19/12 ,  C12P19/26 ,  C12N2500/34 ,  C12R2001/19

摘要： The present invention is in the technical field of synthetic biology and metabolic engineering. More particularly, the present invention is in the technical field of metabolically engineered cells of microorganisms and the use of said cells in a fermentation. The present invention describes a metabolically engineered cell of a microorganism and a method by fermentation with said cell for production of a sialylated di- and/or oligosaccharide. The metabolically engineered cell comprises a pathway for production of said sialylated di- and/or oligosaccharide, synthesizes sialic acid, expresses at least one sialyltransferase, preferably is modified in the expression or activity of at least one sialyltransferase, is modified to have a fully or partially knocked out or rendered less functional sialic acid catabolic pathway and is modified for overexpression of an endogenous sialic acid transporter and/or expression, preferably overexpression, of an exogenous, homologous and/or heterologous sialic acid transporter. Furthermore, the present invention provides for purification of said sialylated di- and/or oligosaccharide from the cultivation, preferably fermentation.

公开(公告)号：US20240183000A1

公开(公告)日：2024-06-06

申请号：US18556302

申请日：2022-04-18

申请人： GABIO BIYOLOJIK URUNLER ANONiM SiRKETi

发明人： Mehmet Sinan OKAY

IPC分类号： C22B1/00 ,  B03B1/04 ,  B03B5/00 ,  C12N1/18

CPC分类号： C22B1/00 ,  B03B1/04 ,  B03B5/00 ,  C12N1/18

摘要： The present invention relates to an environmentally-friendly biochemical composition for use as an additive into an aqueous medium in order to increase the process efficiency and resulting grade in all gravimetric and magnetic wet ore concentration and classification methods that essentially require the use of water, in the mining industry. The invention particularly relates to a biochemical liquid concentrate composition for use as an additive into the existing process or feed water, and a method of use thereof, comprising a fermentation supernatant obtained from the culture of Saccharomyces cerevisiae, one or more surfactants selected from the group consisting of non-ionic surfactants and anionic surfactants, preferably hydrogen peroxide or chlorine dioxide, and urea-based or other suitable preservatives, which increases the process efficiency and grade recovery, without requiring an additional facility investment, in said ore beneficiation processes.

公开(公告)号：US20240174722A1

公开(公告)日：2024-05-30

申请号：US18281117

申请日：2022-03-11

申请人： TENZA, INC.

发明人： Anik Debnath ,  Ameet Shetty

IPC分类号： C07K14/395 ,  A61K36/064 ,  A61K38/00 ,  C12N1/18 ,  C12R1/685 ,  C12R1/84 ,  C12R1/87 ,  C12R1/885

CPC分类号： C07K14/395 ,  A61K36/064 ,  C12N1/185 ,  A61K38/00 ,  C07K2319/02 ,  C12R2001/685 ,  C12R2001/84 ,  C12R2001/87 ,  C12R2001/885

摘要： Provided herein are signal peptides that direct secretion of expressed payload proteins in yeast. Methods of using the signal peptides for therapeutic and non-therapeutic utilities are also provided. Compositions comprising yeast comprising the signal peptides and methods of using said yeast comprising the signal peptides for therapeutic and non-therapeutic utilities are also provided. Methods to design and generate the disclosed signal peptides are also provided.

公开(公告)号：US20230416127A1

公开(公告)日：2023-12-28

申请号：US18460056

申请日：2023-09-01

申请人： Neozyme International, Inc.

发明人： Parker Dale ,  Parker David Dale

IPC分类号： C02F3/34 ,  C12N1/18 ,  C12N1/16 ,  C02F3/02 ,  C02F3/28 ,  A01N63/32 ,  C02F11/04 ,  C12P5/02

CPC分类号： C02F3/347 ,  C12N1/18 ,  C12N1/16 ,  C02F3/02 ,  C02F3/28 ,  A01N63/32 ,  C02F11/04 ,  C12P5/023 ,  C12N2500/74 ,  Y02W10/30 ,  C02F3/342 ,  C02F2101/32

摘要： The present invention provides a process for the treatment of sewage sludge with enzymes, which process comprises treating a sewage sludge resulting from the treatment of municipal or industrial waste water with a composition comprising a fermentation supernatant product from a Saccharomyces cerevisiae culture and a non-ionic surfactant, wherein said fermentation supernatant product is free of active enzymes, at conditions suitable for generating said active enzymes from said sewage sludge in situ.

公开(公告)号：US11814629B2

公开(公告)日：2023-11-14

申请号：US17206900

申请日：2021-03-19

申请人： Lallemand Hungary Liquidity Management LLC

发明人： Aaron Argyros ,  Alexandra-Elena Panaitiu

IPC分类号： C12N9/34 ,  C12N1/18 ,  C12P7/06 ,  C07K14/395 ,  C12N15/81 ,  C07K14/39 ,  C12N9/30 ,  C12R1/865

CPC分类号： C12N15/81 ,  C07K14/39 ,  C12N1/185 ,  C12N9/242 ,  C12R2001/865 ,  C12Y302/01001

摘要： The present disclosure concerns recombinant yeast host cell for saccharification of a biomass. The recombinant yeast host cell has a genetic modification for expressing a heterologous polypeptide having glucoamylase activity (Penicillum oxalicum glucoamylase). In some embodiments, the heterologous polypeptide can comprise a signal sequence. The present disclosure also concerns a process for saccharification of a biomass using the recombinant yeast host cell as well as a process for fermenting the saccharified biomass into a fermentation product.