公开(公告)号：US12127577B2

公开(公告)日：2024-10-29

申请号：US13695672

申请日：2011-05-03

Applicant: Guylaine Lacaze ,  Isabel Trogh ,  Bernard Genot ,  Filip Arnaut

Inventor： Guylaine Lacaze ,  Isabel Trogh ,  Bernard Genot ,  Filip Arnaut

IPC: A23L33/22 ,  A21D2/18 ,  A21D8/04 ,  A21D10/00 ,  A23L7/10 ,  A23L7/104 ,  A23L33/10 ,  C12P19/04

CPC classification number: A23L33/22 ,  A21D2/18 ,  A21D8/045 ,  A21D10/002 ,  A23L7/104 ,  A23L7/115 ,  A23L33/10 ,  C12P19/04

Abstract: A method for preparing a liquid composition having a high arabinoxylan oligosaccharides content (AXOS), the product obtained by this method, and its use in food applications, in particular in baked products.

公开(公告)号：US20240344095A1

公开(公告)日：2024-10-17

申请号：US18633236

申请日：2024-04-11

Applicant: Sanko Tekstil Isletmeleri San. Ve Tic. A.S.

Inventor： Halil Ibrahim AKBAS ,  Mehmet Erkan ÖZSEYHAN ,  Gözde ÖKTEM ,  Melih KANSU ,  Zeynep KARDES ,  Semih KAZANÇ

IPC: C12P19/04 ,  C08J11/06 ,  C12P19/02 ,  C12P19/14

CPC classification number: C12P19/04 ,  C08J11/06 ,  C12P19/02 ,  C12P19/14 ,  C08J2367/00

Abstract: The present invention refer to a process for the production of microbial cellulose using a waste textile material, for example 100% cotton or blended cotton/polyester/elastane waste textile, said waste textile material comprising cellulose, comprising the following steps: providing an amount of said waste textile material; pre-treating said waste textile material with an aqueous solution comprising NaOH and urea, to obtain a pre-treated fabric, wherein said step of treating said waste textile with an aqueous solution comprising NaOH and urea is carried out at a temperature ranging from −25° C. to +30° C.; treating said pre-treated fabric with cellulase enzymes, to convert said cellulose into glucose, whereby a mixture including glucose is obtained; preparing a culture medium comprising said mixture including glucose; inoculating said culture medium with microbial cellulose producing microorganisms; and incubating said microbial cellulose producing microorganisms to obtain microbial cellulose.

公开(公告)号：US20240318214A1

公开(公告)日：2024-09-26

申请号：US18576607

申请日：2022-10-12

Applicant: SYNAURA BIOTECHNOLOGY (SHANGHAI) CO., LTD.

Inventor： Yan WU ,  Jing TANG ,  Jin ZHAO ,  Shu WANG ,  Zhenhua TIAN ,  Fei YAO ,  Miao LI ,  Hong XU ,  Chenxi HUANG ,  Yurou LIU

IPC: C12P19/04 ,  C12N1/20 ,  C12N9/10 ,  C12N15/70 ,  C12P19/18 ,  C12R1/19

CPC classification number: C12P19/04 ,  C12N1/20 ,  C12N9/1051 ,  C12N15/70 ,  C12P19/18 ,  C07K2319/24 ,  C07K2319/35 ,  C12R2001/19 ,  C12Y204/01069

Abstract: A genetically engineered bacterium and a preparation method and use thereof are disclosed. The genetically engineered bacteria contain a gene encoding α-1,2-fucosyltransferase, and a gene encoding a protein tag is connected to the gene encoding α-1,2-fucosyltransferase; the protein tag is MBP, SUMO1, SUMO2 or TrxA, the amino acid sequence of the MBP is shown in SEQ ID NO: 2, the amino acid sequence of the SUMO1 is shown in SEQ ID NO: 3, the amino acid sequence of the SUMO2 is shown in SEQ ID NO: 4, the amino acid sequence of the TrxA is shown in SEQ ID NO: 5. Fermentation with the genetically engineered bacteria can greatly increase the yield of 2′-fucosyllactose compared to the genetically engineered bacteria that only expresses α-1,2-fucosyltransferase exogenously, and the yield can be more than doubled in a preferred case.

公开(公告)号：US20240309415A1

公开(公告)日：2024-09-19

申请号：US18576585

申请日：2022-10-11

Applicant: SYNAURA BIOTECHNOLOGY (SHANGHAI) CO., LTD.

Inventor： Qi JIAO ,  Zhenhua TIAN ,  Shu WANG ,  Zhanbing CHENG ,  Xiaolan XU ,  Fei YAO ,  Miao LI ,  Hong XU ,  Chenxi HUANG ,  Yurou LIU

IPC: C12P19/04 ,  C12N1/20 ,  C12R1/19

CPC classification number: C12P19/04 ,  C12N1/205 ,  C12N2500/12 ,  C12N2500/34 ,  C12N2500/35 ,  C12N2500/74 ,  C12R2001/19

Abstract: The present invention discloses a genetically engineered bacteria, which is E. coli integrated with lysogenic λDE3, and lacZ gene is completely inactivated, but does not affect exogenous protein expression of the genetically engineered bacteria. The present invention also discloses a method for culturing the genetically engineered bacteria, and a method for preparing human milk oligosaccharides using the same, and use of the genetically engineered bacteria. The genetically engineered bacteria of the present invention can efficiently produce human milk oligosaccharides, such as 2′-fucosyllactose, and have wide industrial application prospects.

公开(公告)号：US12091470B2

公开(公告)日：2024-09-17

申请号：US17667707

申请日：2022-02-09

Applicant: NUTRITION & BIOSCIENCES USA 4, INC.

Inventor： Rahul B. Kasat ,  Jayme L. Paullin ,  Andrea M. Perticone ,  T. Joseph Dennes ,  Rakesh Nambiar ,  Michael W. Cobb

IPC: C08B37/00 ,  A61K8/73 ,  C08L5/00 ,  C11D3/22 ,  C12P19/04 ,  D06L1/00 ,  D06L1/12 ,  A61Q11/00

CPC classification number: C08B37/0009 ,  A61K8/73 ,  C08L5/00 ,  C11D3/225 ,  C12P19/04 ,  D06L1/00 ,  D06L1/12 ,  A61K2800/48 ,  A61Q11/00

Abstract: A hydrocolloid or aqueous solution comprising a poly alpha-1,3-glucan ether compound is disclosed having a viscosity of at least about 10 centipoise (cPs). The poly alpha-1,3-glucan ether compound in these compositions has a degree of substitution of about 0.05 to about 3.0. Also disclosed is a method for increasing the viscosity of a hydrocolloid or aqueous composition using a poly alpha-1,3-glucan ether compound.

公开(公告)号：US12049654B2

公开(公告)日：2024-07-30

申请号：US17164536

申请日：2021-02-01

Applicant: NATIONAL TECHNOLOGY & ENGINEERING SOLUTIONS OF SANDIA, LLC

Inventor： Parthasarathi Ramakrishnan ,  Tanmoy Dutta ,  Blake Simmons ,  Seema Singh

IPC: C12P19/04 ,  C08B37/00 ,  C08H8/00 ,  C12P19/02 ,  C13K1/02 ,  C13K13/00

CPC classification number: C12P19/04 ,  C08B37/0057 ,  C08H8/00 ,  C12P19/02 ,  C13K1/02 ,  C12P2201/00 ,  C12P2203/00 ,  C13K13/002

Abstract: Methods and compositions are provided for ionic liquid processing of biomass.

公开(公告)号：US20240229092A1

公开(公告)日：2024-07-11

申请号：US17769331

申请日：2020-12-16

Applicant: ADVENTUS LIFEFOODS INC.

Inventor： Mohammad Hassan QURESHI

IPC: C12P19/18 ,  C07H3/06 ,  C12P19/04 ,  C12P19/14

CPC classification number: C12P19/18 ,  C07H3/06 ,  C12P19/04 ,  C12P19/14

Abstract: A novel protocol is presented for the production of resistant-isomalto-oligosaccharides (“IMO-R”) that are resistant to human gut enzymes, in particular, dextranase, α-glucosidase and stomach and pancreatic α-amylases, thus exhibiting an increasing dietary Fiber effect.

公开(公告)号：US12018306B2

公开(公告)日：2024-06-25

申请号：US18373423

申请日：2023-09-27

Applicant: Jiangnan University

Inventor： Jian Yin ,  Jing Hu ,  Xiaopeng Zou

IPC: C12P19/04

CPC classification number: C12P19/04

Abstract: The disclosure discloses a method for chemically synthesizing a Helicobacter pylori core lipopolysaccharide oligosaccharide antigen carbohydrate chain, and belongs to the technical field of carbohydrate chemistry. The disclosure uses D-glucose, D-galactose and D-mannose as starting materials, which undergo a series of protection and deprotection reactions to prepare eight monosaccharide blocks. The eight monosaccharide block compounds as shown in formulas 2 to 9 undergo glycosylation reactions under catalysis of corresponding activating reagents, to prepare H. pylori lipopolysaccharide core oligosaccharide antigen fragments as shown in formula 1. The disclosure further combines the synthesized oligosaccharide fragments with a chip to make a carbohydrate chip, screens the optimal antigen fragments using patient serum, or combines the synthesized oligosaccharide fragments with carrier proteins to make glycoconjugates.

公开(公告)号：US12012622B1

公开(公告)日：2024-06-18

申请号：US17045149

申请日：2019-03-28

Applicant: GODO SHUSEI CO., LTD.

Inventor： Rei Odaka ,  Yasuhiro Baba ,  Junki Ogasawara ,  Jun Yoshikawa

IPC: C12P19/04 ,  C12N9/24

CPC classification number: C12N9/2402 ,  C12P19/04 ,  C12Y302/01108

Abstract: [Problem] To provide: a novel enzyme which is derived from a microorganism other than Bacillus circulans and is highly selective for galactotrisaccharide production and with which the galactooligosaccharide can be highly efficiently produced; and a novel method capable of producing the galactooligosaccharide. [Solution] A method for producing a galatooligosaccharide, the method including bringing an enzyme having an amino acid sequence selected from the group consisting of (a) to (f) and cells of a bacterium belonging to the genus Paenibacillus and/or a galactooligosaccharide-producing enzyme for the bacterium, into contact with lactose. (a) An amino acid sequence of sequence number 1. (b) An amino acid sequence of sequence number 2. (c) An amino acid sequence of an enzyme having galactooligosaccharide-producing activity, the amino acid sequence being the amino acid sequence of sequence number 1 in which one to ten amino acids have been replaced, removed, or inserted. (d) An amino acid sequence of an enzyme having galactooligosaccharide-producing activity, the amino acid sequence being the amino acid sequence of sequence number 2 in which one to ten amino acids have been replaced, removed, or inserted. (e) An amino acid sequence of an enzyme having galactooligosaccharide-producing activity, the amino acid sequence having a homology of 80% or higher but less than 100% to the amino acid sequence of sequence number 1. (f) An amino acid sequence of an enzyme having galactooligosaccharide-producing activity, the amino acid sequence having a homology of 80% or higher but less than 100% to the amino acid sequence of sequence number 2.

公开(公告)号：US20240158601A1

公开(公告)日：2024-05-16

申请号：US18530194

申请日：2023-12-05

Applicant: The Regents of the University of Colorado, a body corporate

Inventor： Andrew J. Hess ,  Ivan I. Smalyukh ,  Qingkun Liu ,  Joshua A. De La Cruz ,  Blaise Fleury ,  Eldho Abraham ,  Vladyslav Cherpak ,  Bohdan Senyuk

IPC: C08J9/28 ,  C08J9/00 ,  C12P19/04

CPC classification number: C08J9/28 ,  C08J9/0061 ,  C12P19/04 ,  B32B23/048 ,  B32B2262/062 ,  B32B2266/126 ,  C08J2201/0504 ,  C08J2201/0546 ,  C08J2205/022 ,  C08J2205/026 ,  C08J2301/02 ,  C08J2383/04

Abstract: Disclosed are cellulose-based flexible gels containing cellulose nanorods, ribbons, fibers, and the like, and cellulose-enabled inorganic or polymeric composites, wherein the gels have tunable optical, heat transfer, and stiffness properties. The disclosed gels are in the form of hydrogels, organogels, liquid-crystal (LC) gels, and aerogels. Further disclosed are highly transparent and flexible cellulose nanofiber-polysiloxane composite aerogels featuring enhanced mechanical robustness, tunable optical anisotropy, and low thermal conductivity. Further disclosed are gels comprising cellulosic material derived from bacteria and processes for preparing bacterial cellulose gels and methods of use.