公开(公告)号：US12110347B2

公开(公告)日：2024-10-08

申请号：US17680581

申请日：2022-02-25

Applicant: NUTRITION & BIOSCIENCES USA 4, INC.

Inventor： Qiong Cheng ,  Robert DiCosimo ,  Arthur Ouwehand ,  Zheng You ,  Mark S. Payne ,  Jian Ping Lai ,  Kristin Ruebling-Jass ,  Steven Cary Rothman

IPC: C08B37/00 ,  A21D13/45 ,  A21D13/80 ,  A23C9/13 ,  A23G3/36 ,  A23G9/32 ,  A23L2/52 ,  A23L7/126 ,  A23L7/135 ,  A23L21/10 ,  A23L29/30 ,  A23L33/21 ,  A61K31/716 ,  C08L5/00 ,  C12N9/10 ,  C12N9/44 ,  C12P19/04 ,  C12P19/08 ,  C12P19/18

CPC classification number: C08B37/0009 ,  A21D13/45 ,  A21D13/80 ,  A23C9/13 ,  A23G3/36 ,  A23G9/32 ,  A23L2/52 ,  A23L7/126 ,  A23L7/135 ,  A23L21/10 ,  A23L29/37 ,  A23L33/21 ,  A61K31/716 ,  C08L5/00 ,  C12N9/1051 ,  C12N9/246 ,  C12P19/04 ,  C12P19/08 ,  C12P19/18 ,  C12Y204/00 ,  C12Y204/01002 ,  C12Y204/01005 ,  C12Y302/01 ,  C12Y302/01068 ,  A23V2002/00 ,  C08L2203/02 ,  C08L2203/12 ,  C08L2205/16 ,  A61K31/716 ,  A61K2300/00

Abstract: An enzymatically produced soluble α-glucan fiber composition is provided suitable for use as a digestion resistant fiber in food and feed applications. The soluble α-glucan fiber composition can be blended with one or more additional food ingredients to produce fiber-containing compositions. Methods for the production and use of compositions comprising the soluble α-glucan fiber are also provided.

公开(公告)号：US20240327886A1

公开(公告)日：2024-10-03

申请号：US18561233

申请日：2022-05-17

Applicant: DSM IP Assets B.V.

Inventor： Manos PAPADAKIS ,  Ted JOHANSON ,  Peter BECKER

IPC: C12P19/04 ,  C07K14/245 ,  C12N1/20 ,  C12N9/10 ,  C12N15/52 ,  C12P19/18 ,  C12R1/19

CPC classification number: C12P19/04 ,  C07K14/245 ,  C12N1/20 ,  C12N9/1051 ,  C12N15/52 ,  C12P19/18 ,  C12R2001/19 ,  C12Y204/01062 ,  C12Y204/01069 ,  C12Y204/01222

Abstract: This invention relates to a method of producing mixtures of various human milk oligosaccharides (HMOs) with unique HMO blend profiles, consisting predominantly of LNFP-I and LNT and of other HMOs in less significant amounts. The less abundant HMOs might be 2′-FL, LNT-II or DFL. The strategies for achieving specific HMO blends include strain engineering and fermentation methods.

公开(公告)号：US20240318213A1

公开(公告)日：2024-09-26

申请号：US18547082

申请日：2022-02-18

Applicant: KYOWA HAKKO BIO CO., LTD.

Inventor： Kenichiro TABATA ,  Nozomu KAMADA

IPC: C12P19/00 ,  C12N9/10 ,  C12P19/18

CPC classification number: C12P19/00 ,  C12N9/1051 ,  C12P19/18

Abstract: An object of the present invention is to provide a modified protein having an α1,2-fucosyltransferase activity and a method for producing a fucose-containing carbohydrate using the protein or a microorganism having an ability to produce the protein. According to the present invention, a fucose-containing carbohydrate such as 2′-fucosyllactose can be more efficiently produced by using a protein having an α1,2-fucosyltransferase activity and being modified to substitute a specific amino acid residue with another amino acid residue, or a microorganism having the protein or an ability to produce the protein, compared to the case of using a wild-type protein having an α1,2-fucosyltransferase activity, or a microorganism having the protein or an ability to produce the protein.

公开(公告)号：US20240287479A1

公开(公告)日：2024-08-29

申请号：US18574052

申请日：2022-07-29

Applicant: XINGHUA GL STEVIA CO., LTD

Inventor： Boya ZHOU ,  Honghua JIA ,  Jing WANG ,  Yan LI ,  Yehui TAO ,  Jie YU ,  Lei LIN ,  Ruiqi MA

IPC: C12N9/10 ,  C12N15/70 ,  C12P19/18

CPC classification number: C12N9/1051 ,  C12N9/1062 ,  C12N15/70 ,  C12P19/18 ,  C12Y204/01

Abstract: A glycosyltransferase mutant and a method for catalytic synthesis of rebaudioside M using the glycosyltransferase mutant are provided. The glycosyltransferase mutant is obtained through mutation based on a glycosyltransferase amino acid sequence shown in SEQ ID NO: 1, and can be produced by inducing expression in a mutant strain. The glycosyltransferase mutant can be used as a catalyst for enzymatic catalytic synthesis of 12.8 g/LRebM from 20 g/LRebE. Michaelis constants (a kinetic parameter) of the glycosyltransferase mutant S195Q for rebaudioside E and rebaudioside D are 56.34±2.02 μM and 214.48±14.54 μM, respectively, which are ⅓ and ⅖ of a Michaelis constant of a wild-type (WT) glycosyltransferase, respectively. The glycosyltransferase mutant can be coupled with sucrose synthase (SuSy) to allow the catalytic synthesis of rebaudioside M and a recombinant strain in which a glycosyltransferase UGT76G1 or a mutant thereof is co-expressed with SuSy is constructed to allow the catalytic synthesis of rebaudioside M.

公开(公告)号：US12065462B2

公开(公告)日：2024-08-20

申请号：US16626217

申请日：2018-06-27

Applicant: GLYCOM A/S

Inventor： Nikolay Khanzhin

IPC: C07H1/06 ,  A23L33/00 ,  B01D61/02 ,  B01D61/04 ,  B01D61/14 ,  B01D61/58 ,  B01D69/02 ,  B01D69/12 ,  B01D71/56 ,  B01D71/58 ,  C07H3/06 ,  C08B37/00 ,  C12P19/04 ,  C12P19/12 ,  C12P19/18

CPC classification number: C07H1/06 ,  A23L33/40 ,  B01D61/027 ,  B01D61/04 ,  B01D61/146 ,  B01D61/58 ,  B01D69/02 ,  B01D69/12 ,  B01D71/56 ,  B01D71/58 ,  C07H3/06 ,  C08B37/0003 ,  C12P19/04 ,  C12P19/12 ,  C12P19/18 ,  A23L33/00 ,  A23V2002/00 ,  B01D61/145 ,  B01D2315/16 ,  B01D2325/20

Abstract: The invention relates to a method for separating sialylated oligosaccharides, preferably sialylated human milk oligosaccharides (HMOs), from disaccharides, preferably lactose, produced by a fermentation or enzymatic process.

公开(公告)号：US12060593B2

公开(公告)日：2024-08-13

申请号：US16628516

申请日：2018-07-06

Applicant: Chr. Hansen HMO GmbH

Inventor： Stefan Jennewein ,  Katja Parschat

IPC: C12P19/18 ,  A23L33/00 ,  A23L33/125 ,  A23L33/21 ,  C12N1/21 ,  C12N9/10 ,  C12N15/63

CPC classification number: C12P19/18 ,  A23L33/125 ,  A23L33/21 ,  A23L33/40 ,  C12N9/1051 ,  C12N15/63 ,  C12Y204/01065

Abstract: Fucosyltransferases capable of transferring a fucose residue from a donor substrate to a lactotetraose, methods for producing fucosylated oligosaccharides utilizing the fucosyltransferases, and the use of the thus produced fucosylated oligosaccharides for manufacturing nutritional compositions are provided.

公开(公告)号：US12012626B2

公开(公告)日：2024-06-18

申请号：US17546758

申请日：2021-12-09

Applicant: Manus Bio Inc.

Inventor： Ajikumar Parayil Kumaran ,  Christine Nicole S. Santos ,  Jason Eric Donald ,  Mary Elizabeth Fowler ,  Ryan N. Philippe ,  Christopher Scott Frei ,  Aaron Love

IPC: C12P19/18

CPC classification number: C12P19/18

Abstract: In various aspects and embodiments, the invention provides microbial cells and methods for producing advanced glycosylation products from lower glycosylated intermediates. The microbial cell expresses one or more UDP-dependent glycosyl transferase enzymes in the cytoplasm, for glycosylation of the intermediates. When incubating the microbial strain with a plant extract or fraction thereof comprising the intermediates, these glycosylated intermediates are available for further glycosylation by the cell, and the advanced glycosylation products can be recovered from the media and/or microbial cells.

公开(公告)号：US20240148036A1

公开(公告)日：2024-05-09

申请号：US18544879

申请日：2023-12-19

Applicant: PURECIRCLE SDN BHD

Inventor： Avetik MARKOSYAN

IPC: A23L27/30 ,  A21D2/18 ,  A21D2/36 ,  A21D13/062 ,  A21D13/80 ,  A23C9/13 ,  A23L2/02 ,  A23L2/54 ,  A23L2/56 ,  A23L2/60 ,  A23L27/00 ,  A61K36/28 ,  C12P19/14 ,  C12P19/18 ,  C12P19/56

CPC classification number: A23L27/35 ,  A21D2/18 ,  A21D2/36 ,  A21D13/062 ,  A21D13/80 ,  A23C9/1307 ,  A23L2/02 ,  A23L2/54 ,  A23L2/56 ,  A23L2/60 ,  A23L27/33 ,  A23L27/36 ,  A23L27/88 ,  A61K36/28 ,  C12P19/14 ,  C12P19/18 ,  C12P19/56 ,  C12Y204/01019 ,  C12Y302/01001 ,  A23V2002/00 ,  Y02P20/52

Abstract: Glucosyl stevia compositions are prepared from steviol glycosides of Stevia rebaudiana Bertoni. The glucosylation was performed by cyclodextrin glucanotransferase using the starch as source of glucose residues. The glucosyl stevia compositions were purified to >95% content of total steviol glycosides. The compositions can be used as sweetness enhancers, flavors, flavor enhancers and sweeteners in foods, beverages, cosmetics and pharmaceuticals.

公开(公告)号：US20240141307A1

公开(公告)日：2024-05-02

申请号：US18177070

申请日：2023-03-01

Applicant: DSM IP Assets B.V.

Inventor： Manos PAPADAKIS

IPC: C12N9/10 ,  C07K14/24 ,  C12P19/04 ,  C12P19/18

CPC classification number: C12N9/1081 ,  C07K14/24 ,  C12P19/04 ,  C12P19/18 ,  C12Y204/99004

Abstract: The present disclosure relates to the production of sialylated Human Milk Oligosaccharides (HMOs), in particular to the biosynthetic production of 3′-sialyllactose (3′SL), and to genetically engineered cells and methods suitable for said production.

公开(公告)号：US20240132929A1

公开(公告)日：2024-04-25

申请号：US18302932

申请日：2023-04-18

Applicant: Conagen Inc.

Inventor： Guohong Mao ,  Jacob Edward Vick ,  Michael Batten ,  David Byun ,  Yang Luo ,  Yilin Wu ,  Beihua Zhang ,  Xiaodan Yu

IPC: C12P19/56 ,  A21D13/062 ,  A23G4/10 ,  A23L2/60 ,  A23L27/30 ,  C07H1/00 ,  C07H15/24 ,  C07H15/256 ,  C12N9/10 ,  C12N9/42 ,  C12N15/74 ,  C12N15/81 ,  C12N15/82 ,  C12P19/18 ,  C12P19/44

CPC classification number: C12P19/56 ,  A21D13/062 ,  A23G4/10 ,  A23L2/60 ,  A23L27/36 ,  C07H1/00 ,  C07H15/24 ,  C07H15/256 ,  C12N9/1062 ,  C12N9/1081 ,  C12N9/2445 ,  C12N15/74 ,  C12N15/81 ,  C12N15/8243 ,  C12N15/8245 ,  C12P19/18 ,  C12P19/44 ,  A23K20/163 ,  A23V2002/00

Abstract: The present invention relates to the production of steviol glycoside rebaudiosides D4, WB1 and WB2 and the production of rebaudioside M from Reb D4.