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公开(公告)号：US20230348944A1
公开(公告)日：2023-11-02
申请号：US17785843
申请日：2020-12-16
申请人： INBIOSE N.V.
CPC分类号： C12P19/18 , C12Y204/01069 , C12N9/1051 , C12N1/205 , C12N15/70 , C12R2001/19
摘要： The present invention relates to methods for producing 2′ fucosyllactose (2′-FL), as well as newly identified fucosyltransferases, more specifically newly identified lactose binding α-1,2-fucosyltransferase polypeptides, and their applications. Furthermore, the present invention provides methods for producing 2-fucosyllactose (2′FL) using the newly identified α-1,2-fucosyltransferases.
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2.发明申请公开(公告)号：US20170081689A1
公开(公告)日：2017-03-23
申请号：US14708568
申请日：2015-05-11
申请人： Glycosyn LLC
CPC分类号： C12P19/18 , C12N9/1051 , C12N15/52 , C12N15/72 , C12Y204/01069 , C40B30/02 , G06F19/16
摘要： The invention provides compositions and methods for engineering E. coli or other host production bacterial strains to produce fucosylated oligosaccharides, and the use thereof in the prevention or treatment of infection.
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3.发明授权Alpha (1,2) fucosyltransferases suitable for use in the production of fucosylated oligosaccharides 有权适用于生产岩藻糖基化寡糖的α（1,2）岩藻糖基转移酶公开(公告)号：US09029136B2
公开(公告)日：2015-05-12
申请号：US13557655
申请日：2012-07-25
CPC分类号： C12P19/18 , C12N9/1051 , C12N15/52 , C12N15/72 , C12Y204/01069 , C40B30/02 , G06F19/16
摘要： The invention provides compositions and methods for engineering E. coli or other host production bacterial strains to produce fucosylated oligosaccharides, and the use thereof in the prevention or treatment of infection.
摘要翻译： 本发明提供用于制备大肠杆菌或其他宿主生产细菌菌株以产生岩藻糖基化寡糖的组合物和方法，以及其在预防或治疗感染中的用途。
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公开(公告)号：US20230193334A1
公开(公告)日：2023-06-22
申请号：US18000246
申请日：2022-11-01
发明人： Chul Soo SHIN , Jong Won YOON , Young Ha SONG , Jong-GIl YOO , Young Sun Yoo , Heon-Hak Lee
CPC分类号： C12P19/00 , C12N9/2402 , C12Y302/01023 , C12N9/1051 , C12Y204/01069
摘要： Disclosed is a method for improving the productivity of 2′-fucosyllactose (2′-FL) through enzymatic treatment. Lactose used as a substrate in the stationary phase during culture is degraded by treatment with a small amount of enzyme, the resulting glucose is consumed to produce guanosine diphosphate-L-fucose as a precursor of 2′-fucosyllactose, and the use of lactose left after culture can be maximally utilized for the production of 2′-fucosyllactose. As a result, it is possible to increase the productivity of 2′-fucosyllactose in an economically efficient manner because additional glucose is not required while minimizing by-products.
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公开(公告)号：US09701992B2
公开(公告)日：2017-07-11
申请号：US13809340
申请日：2011-07-12
申请人： Jo Maertens , Joeri Beauprez , Marjan De Mey
发明人： Jo Maertens , Joeri Beauprez , Marjan De Mey
IPC分类号： C12P19/14 , C12P19/18 , C12N15/70 , C12N15/81 , C12N1/21 , C12N1/19 , C12P19/30 , C12N15/52 , C12N9/10 , C12N9/26 , C12N9/90
CPC分类号： C12P19/14 , C12N9/1051 , C12N9/2431 , C12N9/90 , C12N15/52 , C12N15/70 , C12N15/81 , C12P19/18 , C12P19/305 , C12Y204/01007 , C12Y204/01008 , C12Y204/01022 , C12Y204/01069 , C12Y302/01026 , C12Y504/02002
摘要： The present invention relates to genetically engineered organisms, especially microorganisms such as bacteria and yeasts, for the production of added value bio-products such as specialty saccharide, activated saccharide, nucleoside, glycoside, glycolipid or glycoprotein. More specifically, the present invention relates to host cells that are metabolically engineered so that they can produce said valuable specialty products in large quantities and at a high rate by bypassing classical technical problems that occur in biocatalytical or fermentative production processes.
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6.发明申请公开(公告)号：US20170081353A1
公开(公告)日：2017-03-23
申请号：US15307914
申请日：2015-05-14
申请人： Glycosyn LLC
CPC分类号： C07H13/04 , C12N9/1051 , C12N15/70 , C12P19/00 , C12P19/04 , C12P19/18 , C12Y204/01069
摘要： The invention provides compositions and methods for engineering E. coli or other host production bacterial strains to produce fucosylated oligosaccharides, and the use thereof in the prevention or treatment of infection.
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公开(公告)号：US07214517B2
公开(公告)日：2007-05-08
申请号：US10487357
申请日：2002-08-21
申请人： Nozomu Kamada , Satoshi Koizumi
发明人： Nozomu Kamada , Satoshi Koizumi
CPC分类号： C12N9/1051 , C12P21/005 , C12Y204/01069
摘要： The present invention provides a protein having α1,2-fucosyltransferase activity, a DNA encoding the protein, a recombinant DNA comprising the DNA, a transformant comprising the recombinant DNA, a process for producing the protein having α1,2-fucosyltransferase activity using the transformant, and a process for producing a fucose-containing complex carbohydrate using the transformant.
摘要翻译： 本发明提供具有α2-岩藻糖基转移酶活性的蛋白质，编码该蛋白质的DNA，包含该DNA的重组DNA，包含该重组DNA的转化体，使用该转化体产生具有α2-岩藻糖基转移酶活性的蛋白质的方法 ，以及使用该转化体生产含岩藻糖的复合碳水化合物的方法。
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公开(公告)号：US20240327888A1
公开(公告)日：2024-10-03
申请号：US18579664
申请日：2022-07-15
申请人： INBIOSE N.V.
CPC分类号： C12P19/26 , C12N9/1051 , C12N15/63 , C12Y204/01069
摘要： The present invention is in the technical field of synthetic biology and metabolic engineering. More particularly, the present invention is in the technical field of metabolically engineered cells and use of said cells in a cultivation, preferably a fermentation. The present invention describes a cell and a method for production of a compound. The cell expresses an alpha-1,2-fucosyltransferase that has galactoside alpha-1,2-fucosyltransferase activity on the galactose residue of Gal-b1,3-GlcNAc (LNB, lacto-N-biose). Furthermore, the present invention provides for purification of said compound from the cultivation.
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公开(公告)号：US20240318214A1
公开(公告)日：2024-09-26
申请号：US18576607
申请日：2022-10-12
发明人： Yan WU , Jing TANG , Jin ZHAO , Shu WANG , Zhenhua TIAN , Fei YAO , Miao LI , Hong XU , Chenxi HUANG , Yurou LIU
CPC分类号： C12P19/04 , C12N1/20 , C12N9/1051 , C12N15/70 , C12P19/18 , C07K2319/24 , C07K2319/35 , C12R2001/19 , C12Y204/01069
摘要： A genetically engineered bacterium and a preparation method and use thereof are disclosed. The genetically engineered bacteria contain a gene encoding α-1,2-fucosyltransferase, and a gene encoding a protein tag is connected to the gene encoding α-1,2-fucosyltransferase; the protein tag is MBP, SUMO1, SUMO2 or TrxA, the amino acid sequence of the MBP is shown in SEQ ID NO: 2, the amino acid sequence of the SUMO1 is shown in SEQ ID NO: 3, the amino acid sequence of the SUMO2 is shown in SEQ ID NO: 4, the amino acid sequence of the TrxA is shown in SEQ ID NO: 5. Fermentation with the genetically engineered bacteria can greatly increase the yield of 2′-fucosyllactose compared to the genetically engineered bacteria that only expresses α-1,2-fucosyltransferase exogenously, and the yield can be more than doubled in a preferred case.
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公开(公告)号：US12060594B2
公开(公告)日：2024-08-13
申请号：US16895871
申请日：2020-06-08
申请人： Glycom A/S
发明人： Gyula Dekany , Györgyi Osztrovszky , Martin Matwiejuk , Pierre Chassagne , Markus Jondelius Hederos
CPC分类号： C12P19/18 , C07H1/06 , C07H3/06 , C12Y204/01069
摘要： The invention relates to a method for selective crystallization of 2′-fucosyllactose (2′-FL) from an aqueous solution comprising 2′-FL and one or more other fucosylated carbohydrates by adding acetic acid to the solution. The aqueous solution is separated from an aqueous culture medium prior to crystallization.
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