公开(公告)号：US12123040B2

公开(公告)日：2024-10-22

申请号：US17814337

申请日：2022-07-22

申请人： Chr. Hansen HMO GmbH

发明人： Stefan Jennewein

IPC分类号： C12P19/26 ,  C07H1/00 ,  C07H1/06 ,  C12N1/20 ,  C12N15/72 ,  C12P19/00 ,  C12P19/12 ,  C12P19/14 ,  C12R1/19

CPC分类号： C12P19/26 ,  C07H1/00 ,  C07H1/06 ,  C12N1/205 ,  C12N15/72 ,  C12P19/00 ,  C12P19/12 ,  C12P19/14 ,  C12Y302/01 ,  C12Y302/01023 ,  C12N2800/101 ,  C12R2001/19

摘要： The present invention relates to the use of one or more glycosidases in the process for the production and/or purification of a produced desired oligosaccharide. The process is preferably a microbial fermentation process using a host microorganism, which may also comprise nucleic acids expressing sugar catabolic pathway proteins suitable for the degradation of saccharides otherwise hindering the purification of the desired oligosaccharide.

公开(公告)号：US20240344241A1

公开(公告)日：2024-10-17

申请号：US18701094

申请日：2022-10-14

申请人： THE UNIVERSITY OF QUEENSLAND ,  The University of Tokyo

发明人： David James CRAIK ,  Simon John DE VEER ,  Yen-Hua HUANG ,  Joakim Erik SWEDBERG ,  Hiroaki SUGA ,  Wenyu LIU ,  Toby PASSIOURA

IPC分类号： C40B30/04 ,  C12N15/10 ,  C12Q1/02 ,  C12R1/19 ,  G01N33/68

CPC分类号： C40B30/04 ,  C12N15/1062 ,  C12Q1/02 ,  G01N33/6845 ,  C12R2001/19 ,  G01N2500/04

摘要： Disclosed are proteinaceous coagulation factor XIIa (FXIIa) inhibitors and their use for treating or inhibiting the development of a condition in which inhibiting FXIIa stimulates or effects treatment or inhibition of the development of the condition. Suitable conditions include thromboembolism-associated conditions such as acute coronary syndrome, stroke, deep vein thrombosis and pulmonary embolism, a thrombosis, a thrombosis-associated hematologic disorder such as sickle cell disease or thrombophilia, and an inflammatory condition or a condition related to the kallikrein-kinin system such as hereditary angioedema, multiple sclerosis, rheumatoid arthritis or lupus. The proteinaceous FXIIa inhibitors are also useful for treating or inhibiting thrombus and/or embolus formation. In vitro methods for identifying a disulfide rich peptide which binds to a target substance are also disclosed.

公开(公告)号：US20240327886A1

公开(公告)日：2024-10-03

申请号：US18561233

申请日：2022-05-17

申请人： DSM IP Assets B.V.

发明人： Manos PAPADAKIS ,  Ted JOHANSON ,  Peter BECKER

IPC分类号： C12P19/04 ,  C07K14/245 ,  C12N1/20 ,  C12N9/10 ,  C12N15/52 ,  C12P19/18 ,  C12R1/19

CPC分类号： C12P19/04 ,  C07K14/245 ,  C12N1/20 ,  C12N9/1051 ,  C12N15/52 ,  C12P19/18 ,  C12R2001/19 ,  C12Y204/01062 ,  C12Y204/01069 ,  C12Y204/01222

摘要： This invention relates to a method of producing mixtures of various human milk oligosaccharides (HMOs) with unique HMO blend profiles, consisting predominantly of LNFP-I and LNT and of other HMOs in less significant amounts. The less abundant HMOs might be 2′-FL, LNT-II or DFL. The strategies for achieving specific HMO blends include strain engineering and fermentation methods.

公开(公告)号：US20240271119A1

公开(公告)日：2024-08-15

申请号：US18292421

申请日：2022-07-27

申请人： The Broad Institute, Inc. ,  President and Fellows of Harvard College

发明人： David R. Liu ,  Tina Wang ,  Mary S. Morrison

IPC分类号： C12N15/10 ,  C12M1/00 ,  C12M1/34 ,  C12N1/20 ,  C12N7/00 ,  C12N15/70 ,  C12R1/19

CPC分类号： C12N15/1024 ,  C12M23/58 ,  C12M29/18 ,  C12M41/36 ,  C12N1/205 ,  C12N7/00 ,  C12N15/1058 ,  C12N15/70 ,  C12R2001/19

摘要： Aspects of the disclosure relate to compositions, systems, and methods for evolving nucleic acids and proteins utilizing continuous directed evolution in the periplasm of a host cell. In some embodiments, the methods comprise passing a nucleic acid from cell-to-cell in a desired, function dependent manner. The linkage of the desired function and passage of the nucleic acid from cell-to-cell allows for continuous selection and mutation of the nucleic acid.

公开(公告)号：US12037621B2

公开(公告)日：2024-07-16

申请号：US17476162

申请日：2021-09-15

申请人： Archer Daniels Midland Company

发明人： Benjamin Mason ,  Jason Helvey

IPC分类号： C12P13/08 ,  C07K14/245 ,  C12N1/20 ,  C12N9/00 ,  C12N9/80 ,  C12N9/88 ,  C12R1/19

CPC分类号： C12P13/08 ,  C07K14/245 ,  C12N1/205 ,  C12N9/80 ,  C12N9/88 ,  C12N9/93 ,  C12R2001/19 ,  C12Y305/01003 ,  C12Y403/01019 ,  C12Y604/01001

摘要： Improved production of threonine from E. coli by fermentation is accomplished by attenuation but not elimination of the expression of either or both of the yajV gene encoding omega-amidase (a.k.a. 2-oxoglutaramate amidase) and the ilvA gene encoding threonine dehydratase (a.k.a threonine deaminase). In cases where there is attenuated expression of the ilvA gene, there is no need to express an exogenous cimA gene. In examples of both cases, attenuation is accomplished by engineering these genes to contain a weaker ribosome site. Further improvements in threonine production are made by expression of a heterologous pyruvate carboxylase gene exemplified by expression of the Corynebacterium glutamicum pyc gene under control of an E. coli promoter, to provide expression of pyruvate carboxylase that is not naturally expressed in E. coli.

公开(公告)号：US12031122B2

公开(公告)日：2024-07-09

申请号：US18319442

申请日：2023-05-17

申请人： GENECIS BIOINDUSTRIES INC.

发明人： Adam William Westbrook

IPC分类号： C12N1/20 ,  C07K14/21 ,  C12N1/21 ,  C12N9/04 ,  C12N9/10 ,  C12N9/90 ,  C12N15/52 ,  C12P7/52 ,  C12P7/625 ,  C12R1/19

CPC分类号： C12N1/20 ,  C07K14/21 ,  C12N1/205 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/13 ,  C12N9/90 ,  C12N15/52 ,  C12P7/52 ,  C12P7/625 ,  C12Y101/01036 ,  C12Y203/01009 ,  C12Y203/01016 ,  C12Y208/03001 ,  C12Y504/99002 ,  C12R2001/19

摘要： The disclosure provides recombinant bacterial host cells that metabolize and convert glycerol or volatile fatty acids (VFAs) to poly(3-hydroxybutyrate-co-3-hydroxyvalerate) or PHBV. The disclosure further provides methods of producing PHBV using the recombinant bacteria disclosed herein.

公开(公告)号：US20240209037A1

公开(公告)日：2024-06-27

申请号：US18557282

申请日：2022-04-28

申请人： CJ CHEILJEDANG CORPORATION

发明人： Heejung KIM ,  Moo Young JUNG ,  Hye Mi KIM ,  Hyun Ah KIM

IPC分类号： C07K14/245 ,  C12N15/70 ,  C12P13/22 ,  C12R1/19

CPC分类号： C07K14/245 ,  C12N15/70 ,  C12P13/227 ,  C12R2001/19

摘要： Provided are a variant SpoT protein and a method of producing an L-amino acid using the same.

公开(公告)号：US12006526B2

公开(公告)日：2024-06-11

申请号：US16796417

申请日：2020-02-20

申请人： Braskem S.A.

发明人： Daniel Johannes Koch ,  Lucas Pedersen Parizzi ,  Felipe Galzerani

IPC分类号： C12N1/20 ,  C12N15/52 ,  C12P5/02 ,  C12P7/18 ,  C12P7/28 ,  C12P17/02 ,  C12R1/19

CPC分类号： C12P7/18 ,  C12N1/20 ,  C12N15/52 ,  C12P5/026 ,  C12P7/28 ,  C12P17/02 ,  C12N1/205 ,  C12R2001/19 ,  C12Y101/01043 ,  C12Y101/01301 ,  C12Y102/01009 ,  C12Y203/01008 ,  C12Y207/01011 ,  C12Y207/02003 ,  C12Y301/01031 ,  C12Y401/02022 ,  C12Y501/03001 ,  C12Y503/01006 ,  C12Y504/02

摘要： The present application relates to recombinant microorganisms useful in the biosynthesis of monoethylene glycol (MEG), or optionally MEG and one or more co-product, from one or more hexose feedstock. The present application also relates to recombinant microorganisms useful in the biosynthesis of glycolic acid (GA), or optionally GA and one or more co-product, from one or more hexose feedstock. The present application relates to recombinant microorganisms useful in the biosynthesis of xylitol, or optionally xylitol and one or more co-product, from one or more hexose feedstock. Also provided are methods of producing MEG (or GA or xylitol), or optionally MEG (or GA or xylitol) and one or more co-product, from one or more hexose feedstock using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or the products MEG (or GA or xylitol), or optionally MEG (or GA or xylitol) and one or more co-product.

公开(公告)号：US11993795B2

公开(公告)日：2024-05-28

申请号：US17463628

申请日：2021-09-01

申请人： Jiangnan University

发明人： Bo Jiang ,  Tao Zhang ,  Qing Meng ,  Jingjing Chen

IPC分类号： C12N9/88 ,  C12N1/20 ,  C12R1/19

CPC分类号： C12N9/88 ,  C12N1/205 ,  C12R2001/19 ,  C12Y402/02003

摘要： The disclosure discloses an alginate lyase and application thereof, and belongs to the technical field of biology. The alginate lyase provided by the disclosure has high degradation activity, and the enzyme activity reaches 65 U/mg; the alginate lyase is stable in nature, and the enzyme activity remains 98% or higher of the initial enzyme activity after storage at 4° C. for 18 months; and the alginate lyase has high product specificity. The disclosure uses E. coli as a host to express the alginate lyase derived from V. natriegens, the obtained recombinant E. coli can produce the alginate lyase secreted extracellularly in a conventional LB medium without adding an induction substrate sodium alginate, so the downstream processing technology of protein is simplified, and the disclosure has great industrial application potential.

公开(公告)号：US11859173B2

公开(公告)日：2024-01-02

申请号：US17366757

申请日：2021-07-02

申请人： Northeastern University ,  President and Fellows of Harvard College

发明人： Avinash M. Basavanna ,  Anna Duraj-Thatte ,  Neel S. Joshi

IPC分类号： C12N1/20 ,  C12N1/18 ,  C12R1/19

CPC分类号： C12N1/20 ,  C12N1/18 ,  C12R2001/19

摘要： Disclosed are biomaterials, including engineered living materials (ELMs), comprising a plurality of microbial cells, wherein the material has a Young's Modulus of at least 5 Gpa; and methods of fabricating said biomaterials.