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公开(公告)号：US12089619B2
公开(公告)日：2024-09-17
申请号：US17720375
申请日：2022-04-14
发明人： Jian Mao , Shuangping Liu , Zhilei Zhou , Zhongwei Ji , Xiao Han
摘要： The disclosure discloses a Saccharopolyspora composition and its application in foods, and belongs to the technical field of food fermentation. The disclosure screens Saccharopolyspora hirsuta J2 and Saccharopolyspora jiangxiensis J3 that have an effect of reducing biogenic amine content from wheat koji, and the two strains are prepared into a Saccharopolyspora mixed preparation for use in a preparation process of a fermented alcoholic beverage, fermented food or fermented condiment, so that amino acid content and nutritional value of a fermented product can be improved while the biogenic amine content is reduced, thereby achieving effects of enhancing quality of the fermented food and improving safety of the fermented food, and therefore, the Saccharopolyspora composition has a broad application prospect.
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公开(公告)号：US20230193195A1
公开(公告)日：2023-06-22
申请号：US18050085
申请日：2022-10-27
发明人： Jian MAO , Shaungping LIU , Zhilei ZHOU , Zhongwei JI , Xiao HAN , Jing ZHANG
IPC分类号： C12N1/20 , A23L33/135 , A23L33/20 , A24B15/30
CPC分类号： C12N1/205 , A23L33/135 , A23L33/20 , A24B15/307
摘要： The present disclosure discloses Saccharopolyspora and application thereof in reducing biogenic amines, belonging to the technical field of food fermentation. Saccharopolyspora jiangxiensis J3 is screened from wheat koji and has been preserved in China Center for Type Culture Collection (CCTCC) on Apr. 30, 2020, with a preservation number of CCTCC NO: M 2020104. According to the present disclosure, the strain is applied to a food fermentation system, and results show that the strain does not affect normal fermentation of food, and has an effect of reducing the content of biogenic amines in fermented alcoholic drinks, fermented food and fermented condiments, so that the strain has broad application prospects in the field of food brewing.
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公开(公告)号：US20220322714A1
公开(公告)日：2022-10-13
申请号：US17720375
申请日：2022-04-14
发明人： Jian MAO , Shuangping LIU , Zhilei ZHOU , Zhongwei JI , Xiao HAN
摘要： The disclosure discloses a Saccharopolyspora composition and its application in foods, and belongs to the technical field of food fermentation. The disclosure screens Saccharopolyspora hirsuta J2 and Saccharopolyspora jiangxiensis J3 that have an effect of reducing biogenic amine content from wheat koji, and the two strains are prepared into a Saccharopolyspora mixed preparation for use in a preparation process of a fermented alcoholic beverage, fermented food or fermented condiment, so that amino acid content and nutritional value of a fermented product can be improved while the biogenic amine content is reduced, thereby achieving effects of enhancing quality of the fermented food and improving safety of the fermented food, and therefore, the Saccharopolyspora composition has a broad application prospect.
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公开(公告)号：US20220202949A1
公开(公告)日：2022-06-30
申请号：US17551412
申请日：2021-12-15
发明人： Zhaohui HUANG , Zhimeng WU , Liang GONG , Yanchun LI
IPC分类号： A61K47/69 , C12N5/0783 , A61K47/68 , A61P35/00
摘要： Non-natural sugars modified with a bioorthogonal reactive group are added into a culture medium of immune cells such as NK cells to obtain immune cells modified with the bioorthogonal reactive group; and then, under a physiological condition, a targeting ligand, for example, a nanobody, is modified to a surface of each of the immune cells through a bioorthogonal reaction, wherein the targeting ligand has one terminal with a bioorthogonal reactive pairing group which is capable of being matched and connected with the bioorthogonal reactive group to generate a connecting reaction, connection is implemented by a transpeptidase SrtA-mediated chemoenzymatic method. The targeting ligand highly specifically recognizes and binds to a highly expressed receptor on the surface of tumor cells. The immune cell modified with the targeting ligand can specifically bind in a targeted way to the tumor cells, therefore generating cytokines, killing and damaging tumor cells.
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公开(公告)号：US12123134B2
公开(公告)日：2024-10-22
申请号：US18106685
申请日：2023-02-07
申请人： Jiangnan University
发明人： Qiang Wang , Yimin Wei , Ping Wang , Yuanyuan Yu , Man Zhou , Zhe Jiang
CPC分类号： D06P3/023 , C09B49/04 , C09B67/0014 , C09B67/0082 , D06P1/928
摘要： The present disclosure discloses application of a deep eutectic solvent in textile dyeing, belonging to the technical field of ecological dyeing and finishing of textiles. A natural hydrophobic deep eutectic solvent system (HDES) is first used as a dyeing medium to perform reactive dyeing on cotton. Firstly, the prepared natural hydrophobic deep eutectic solvent is mixed with a small amount of dye aqueous solution at high speed to prepare dye liquor, and then cotton fabric soaked with alkali liquor is put into a dye bath for dyeing. The dyeing method doesn't require addition of inorganic salts and other dyeing auxiliaries, but uses a small amount of water and alkali agent to achieve the same dyeing effect as traditional water bath, and the dyeing medium is safe and environment-friendly. After dyeing, cyclic dyeing can be achieved by simply separating the dye bath, which truly realizes green dyeing of reactive dyes.
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公开(公告)号：US12123039B2
公开(公告)日：2024-10-22
申请号：US17520811
申请日：2021-11-08
申请人： Jiangnan University
发明人： Jing Wu , Wei Xia , Xinghao Xu , Yan Huang
CPC分类号： C12P19/14 , C12N9/2445 , C12Y302/01021
摘要： The disclosure discloses the preparation of thermophilic β-glucosidase and the application thereof, which belongs to the technical field of genetic engineering and fermentation engineering. The present disclosure heterologously expresses the β-glucosidase TpBgl3A derived from thermophilic fungus Talaromyces piceae by constructing a recombinant bacteria. The enzyme production of recombinant bacteria can reach 2324 U/mL in a 3.6 L fermenter. The resulting β-glucosidase TpBgl3A can produce gentioligosaccharides with a high conversion rate using glucose as a substrate at a lower enzyme amount added, which significantly reduces production costs. In the reaction system using glucose and cellobiose as substrates, the conversion rate of gentioligosaccharide reaches 26.2%, which has the potential for industrial production of gentioligosaccharide.
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公开(公告)号：US12123029B2
公开(公告)日：2024-10-22
申请号：US17296218
申请日：2020-07-30
申请人： JIANGNAN UNIVERSITY
发明人： Ye Ni , Zhe Dou , Guochao Xu
摘要： The present invention discloses a cyclohexenecarboxylate ester hydrolase, and a mutant, a coding gene, an expression vector, recombinant bacterium and use thereof. The cyclohexenecarboxylate ester hydrolase AcEst1 and its mutant of the present invention have the function of enantioselectively resolving methyl 3-cyclohexene-1-carboxylate with high efficiency to prepare optically active (S)-3-cyclohexene-1-carboxylic acid. When the substrate concentration is as high as 2000 mM (about 280 g/L), the optical purity of the product is higher than 99%, and the substrate/catalyst is as high as 3500 g/g. As compared with other preparation methods, the product prepared by the method of the present invention has high concentration and high optical purity, the catalytic efficiency is high, the reaction conditions are mild. Furthermore, the method is environmentally friendly, simple in operation and easy for industrial scale-up, thus has a good prospect of application in industry.
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公开(公告)号：US12118896B2
公开(公告)日：2024-10-15
申请号：US16121720
申请日：2018-09-05
申请人： Jiangnan University
发明人： Xiaobei Zhan , Zhitao Li , Minjie Gao , Wenlong Zhang , Zhiyong Zheng , Xingqiao Peng , Yun Jiang
CPC分类号： G09B23/32 , B01L3/5085 , B01L3/561 , B01L3/567 , G09B23/303 , B01L2200/06 , B01L2300/049 , B01L2300/08 , B01L2300/0832 , B01L2300/0874 , B01L2400/0666 , G01N33/02
摘要： The present invention discloses a visual bionic digestive system for a human gastrointestinal tract model, and belongs to the field of bionic digestive systems. The visual bionic digestive system for a human gastrointestinal tract model comprises a reaction system and a control system; the reaction system comprises a bionic stomach system, a bionic small intestine system, a bionic large intestine system and a filtering system; the control system comprises a PLC, peristaltic pumps, and a circulating water tank; the water inlet pipes of the bionic stomach system, the bionic small intestine system, and the bionic large intestine system are connected to a water outlet of the circulating water tank through water pipes; the water outlet pipes of the bionic stomach system, the bionic small intestine system, and the bionic large intestine system are connected to a water inlet of the circulating water tank through water pipes, solenoid valves are arranged on the water pipes, and the solenoid valves on the water pipes are controlled by the PLC. The bionic digestive system provided by the present invention has the advantages of strong visibility, high automation, device modularization, and simple and effective gastrointestinal peristalsis simulation way.
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公开(公告)号：US20240335483A1
公开(公告)日：2024-10-10
申请号：US18579644
申请日：2022-08-05
申请人： INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE , CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS) , UNIVERSITÉ PARIS CITÉ , JIANGNAN UNIVERSITY
发明人： Julien DIANA , Jia SUN
IPC分类号： A61K35/744 , A61K35/00 , A61P3/10 , A61P37/06 , C07K14/47
CPC分类号： A61K35/744 , A61P3/10 , A61P37/06 , C07K14/4723 , A61K2035/115
摘要： The present invention relates to the treatment of autoimmune diseases. The inventors determined that the cathelicidin related antimicrobial peptide (CRAMP) expression was defective in the colon of newborn NOD mice and that this defect was responsible for early dysbiosis. Dysbiosis stimulated the colonic epithelium to produce type I IFNs that pathologically imprinted the local immune system during the pre-weaning period. This miseducation of the immune system promoted the pancreatic autoimmune response and the development of diabetes. Increasing colonic CRAMP expression in newborn NOD mice, by local CRAMP treatment or by CRAMP-expressing probiotic, restored colonic homeostasis, and halted the diabetogenic response preventing autoimmune diabetes. Thus, they identified whether a defective colonic expression in the CRAMP antimicrobial peptide promotes autoimmunity in the pancreas. The use of CRAMP-expressing probiotic can be very helpful to treat autoimmune diseases and particularly autoimmune type 1 diabetes or obesity. Thus, the present invention relates to a recombinant CRAMP-expressing food-grade bacterium and its use in the treatment of autoimmune diseases.
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公开(公告)号：US20240317898A1
公开(公告)日：2024-09-26
申请号：US18678247
申请日：2024-05-30
发明人： Aiquan JIAO , Qing Liu , Zhengyu Jin
CPC分类号： C08B30/12 , C12P19/16 , C12Y302/01041
摘要： The present disclosure provides a method for preparing type III resistant starch in an “extrusion-debranching” manner and belongs to the technical field of functional food. The method for preparing type III resistant starch in an “extrusion-debranching” manner comprises the following steps: adding a starch raw material into a twin screw extruder for primary extrusion, collecting extruded, gelatinized and degraded starch, and then drying and crushing same; and fully mixing the crushed gelatinized starch with pullulanase, adding the mixture into the twin screw extruder for secondary extrusion, collecting the extruded debranched starch, then recrystallizing same, centrifuging and water washing same, and collecting the precipitate and drying same to obtain type III resistant starch. The method rapidly and efficiently debranches starch under high substrate starch concentration, increases the content of the type III resistant starch, and solves the problem of low yield efficiency of the type III resistant starch.
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