公开(公告)号：US12123039B2

公开(公告)日：2024-10-22

申请号：US17520811

申请日：2021-11-08

申请人： Jiangnan University

发明人： Jing Wu ,  Wei Xia ,  Xinghao Xu ,  Yan Huang

IPC分类号： C12P19/14 ,  C12N9/42

CPC分类号： C12P19/14 ,  C12N9/2445 ,  C12Y302/01021

摘要： The disclosure discloses the preparation of thermophilic β-glucosidase and the application thereof, which belongs to the technical field of genetic engineering and fermentation engineering. The present disclosure heterologously expresses the β-glucosidase TpBgl3A derived from thermophilic fungus Talaromyces piceae by constructing a recombinant bacteria. The enzyme production of recombinant bacteria can reach 2324 U/mL in a 3.6 L fermenter. The resulting β-glucosidase TpBgl3A can produce gentioligosaccharides with a high conversion rate using glucose as a substrate at a lower enzyme amount added, which significantly reduces production costs. In the reaction system using glucose and cellobiose as substrates, the conversion rate of gentioligosaccharide reaches 26.2%, which has the potential for industrial production of gentioligosaccharide.