公开(公告)号：US20240318216A1

公开(公告)日：2024-09-26

申请号：US18733787

申请日：2024-06-04

Applicant: Chr. Hansen HMO GmbH

Inventor： Stefan Jennewein ,  Dirk Wartenberg

IPC: C12P19/18 ,  C12N9/10 ,  C12N15/70

CPC classification number: C12P19/18 ,  C12N9/1051 ,  C12N15/70 ,  C12Y204/01146

Abstract: The present invention relates to methods for the production of oligosaccharides in genetically modified bacterial host cells, as well as to the genetically modified host cells used in the methods. The genetically modified host cell comprises at least one recombinant glycosyltransferase, and at least one nucleic acid sequence coding for a protein enabling the export of the oligosaccharide.

公开(公告)号：US12037622B2

公开(公告)日：2024-07-16

申请号：US17323737

申请日：2021-05-18

Applicant: Chr. Hansen HMO GmbH

Inventor： Stefan Jennewein ,  Dirk Wartenberg

IPC: C12P19/18 ,  C12N1/20 ,  C12N9/10 ,  C12N15/70

CPC classification number: C12P19/18 ,  C12N9/1051 ,  C12N15/70 ,  C12Y204/01146

Abstract: The present invention relates to methods for the production of oligosaccharides in genetically modified bacterial host cells, as well as to the genetically modified host cells used in the methods. The genetically modified host cell comprises at least one recombinant glycosyltransferase, and at least one nucleic acid sequence coding for a protein enabling the export of the oligosaccharide.

公开(公告)号：US11981947B2

公开(公告)日：2024-05-14

申请号：US17814333

申请日：2022-07-22

Applicant: Chr. Hansen HMO GmbH

Inventor： Stefan Jennewein

IPC: C12P19/12 ,  C07H1/00 ,  C07H1/06 ,  C12N1/20 ,  C12N15/72 ,  C12P19/00 ,  C12P19/14 ,  C12P19/26 ,  C12R1/19

CPC classification number: C12P19/26 ,  C07H1/00 ,  C07H1/06 ,  C12N1/205 ,  C12N15/72 ,  C12P19/00 ,  C12P19/12 ,  C12P19/14 ,  C12Y302/01 ,  C12Y302/01023 ,  C12N2800/101 ,  C12R2001/19

Abstract: The present invention relates to the use of one or more glycosidases in the process for the production and/or purification of a produced desired oligosaccharide. The process is preferably a microbial fermentation process using a host microorganism, which may also comprise nucleic acids expressing sugar catabolic pathway proteins suitable for the degradation of saccharides otherwise hindering the purification of the desired oligosaccharide.

公开(公告)号：US20240110213A1

公开(公告)日：2024-04-04

申请号：US18267958

申请日：2021-12-22

Applicant: Chr. Hansen HMO GmbH

Inventor： Henning Frerigmann ,  Christian Trötschel ,  Dirk Wartenberg ,  Stefan Jennewein

IPC: C12P19/04 ,  C07K14/245 ,  C12N15/52

CPC classification number: C12P19/04 ,  C07K14/245 ,  C12N15/52

Abstract: Disclosed are genetically engineered microbial cells for the production of an oligosaccharide of interest, wherein said microbial cells possess a variant of the E. coli lactose permease LacY which exhibits a reduced transport activity for lactose, and methods for producing an oligosaccharide by using said genetically engineered microbial cells.

公开(公告)号：US20230399669A1

公开(公告)日：2023-12-14

申请号：US18207453

申请日：2023-06-08

Applicant: Chr. Hansen HMO GmbH

Inventor： Stefan Jennewein ,  Dirk Wartenberg ,  Katja Parschat

IPC: C12P19/12 ,  C12N1/20 ,  C12N9/10 ,  C12N9/12 ,  C12N9/16 ,  C12N9/90 ,  C12P19/04 ,  C12P19/18

CPC classification number: C12P19/12 ,  C12N1/20 ,  C12N9/1051 ,  C12N9/1205 ,  C12N9/16 ,  C12N9/90 ,  C12P19/04 ,  C12P19/18 ,  C12N2500/34 ,  C12Y204/01038 ,  C12Y207/01004 ,  C12Y207/01011 ,  C12Y207/01056 ,  C12Y207/07009 ,  C12Y301/03011 ,  C12Y501/03002 ,  C12Y504/02002

Abstract: Disclosed are genetically engineered microbial cells for the production of oligosaccharides comprising a galactose-β1,4-glucose moiety at their reducing end, wherein said microbial cells are able to produce said oligosaccharides in the absence of exogenously added lactose, and a method of producing said oligosaccharides using said microbial cells.

公开(公告)号：US20240200112A1

公开(公告)日：2024-06-20

申请号：US18393339

申请日：2023-12-21

Applicant: Chr. Hansen HMO GmbH

Inventor： Stefan Jennewein ,  Dirk Wartenberg ,  Katja Parschat

IPC: C12P19/18 ,  C07K14/24 ,  C12N9/04 ,  C12N9/10 ,  C12N9/12 ,  C12N9/16 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12N15/70

CPC classification number: C12P19/18 ,  C07K14/24 ,  C12N9/0006 ,  C12N9/1051 ,  C12N9/12 ,  C12N9/1205 ,  C12N9/1241 ,  C12N9/16 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12N15/70 ,  C12Y101/01271 ,  C12Y204/01065 ,  C12Y207/01011 ,  C12Y207/07013 ,  C12Y301/03011 ,  C12Y401/02013 ,  C12Y402/01047 ,  C12Y504/02008

Abstract: The present invention relates to a method for producing fucosylated oligosaccharides by using a recombinant prokaryotic host cell that is cultivated on a gluconeogenic substrate, as well as to the host cell and its use. The host cell is genetically modified in that the activity of a fructose-6-phosphate converting enzyme is abolished or lowered, and the transport of the produced fucosylated oligosaccharide through the cell membrane is facilitated by an exogenous transport protein.

公开(公告)号：US11920173B2

公开(公告)日：2024-03-05

申请号：US16756279

申请日：2018-10-17

Applicant: Chr. Hansen HMO GmbH

Inventor： Stefan Jennewein ,  Dirk Wartenberg

IPC: C12P19/28 ,  A23L33/135 ,  C12N15/52 ,  A23L33/00

CPC classification number: C12P19/28 ,  A23L33/135 ,  A23L33/40

Abstract: Disclosed are non-naturally-occurring microorganisms for the production of N-acetylneuraminic acid, a method for the production of N-acetylneuraminic acid by fermentation of the non-naturally-occurring microorganisms, and nutritional compositions containing N-acetylneuraminic acid which has been produced by fermentation of the non-naturally-occurring microorganisms.

公开(公告)号：US11912735B2

公开(公告)日：2024-02-27

申请号：US16642329

申请日：2018-08-29

Applicant: Chr. Hansen HMO GmbH

Inventor： Stefan Jennewein ,  Markus Helfrich ,  Benedikt Engels

IPC: C07H1/06 ,  A23L33/00 ,  A23L33/125 ,  A23L33/135 ,  C07H3/06

CPC classification number: C07H1/06 ,  A23L33/125 ,  A23L33/135 ,  A23L33/30 ,  C07H3/06

Abstract: Disclosed is a method for purifying sialylated oligosaccharides from a fermentation broth, cell-lysate or biocatalytic reaction mixture for obtaining high amounts of desired sialylated oligosaccharides in high purity. The method is particular suitable for the large-scale economic purification of sialylated human milk oligosaccharides (such as 3′-sialyllactose, 6′-sialyllactose or sialylated lacto-N-tetraose derivatives) from microbial fermentation, using recombinant bacterial cells or yeast cells. The obtained material is of high purity and can be used for food or medical application such like medical nutrition products, infant formula, dietary supplements, general nutrition products (e.g. dairy drinks).

公开(公告)号：US20220369677A1

公开(公告)日：2022-11-24

申请号：US17761249

申请日：2019-09-19

Applicant: Chr. Hansen HMO GmbH

Inventor： Stefan Jennewein

IPC: A23L7/117 ,  A23L19/00 ,  A23L33/15 ,  A23L33/00 ,  A23L33/125

Abstract: Disclosed is a cereal composition which can be prepared instantly with the addition of a liquid, containing at least one human milk oligosaccharide (HMO), and other components which are necessary for the healthy growth and development of a young 5 individual.

公开(公告)号：US12157754B2

公开(公告)日：2024-12-03

申请号：US18195502

申请日：2023-05-10

Applicant: Chr. Hansen HMO GmbH

Inventor： Stefan Jennewein ,  Markus Helfrich

IPC: C07H1/08 ,  C07H3/06 ,  C12N9/10

Abstract: Provided is a method for the purification of lacto-N-neotetraose from other carbohydrates, characterized in that the method comprises the steps of subjecting an aqueous solution containing lacto-N-neotetraose to two membrane filtration steps using different membranes or of subjecting an aqueous solution containing lacto-N-neotetraose to a membrane filtration step and a continuous chromatography.