公开(公告)号：US12077793B2

公开(公告)日：2024-09-03

申请号：US17445708

申请日：2021-08-23

申请人： University of Maryland, Baltimore ,  UNIVERSITY OF WASHINGTON

发明人： Robert K. Ernst ,  Mark Pelletier ,  Adeline Hajjar

IPC分类号： C07H11/04 ,  A61K31/739 ,  A61K39/02 ,  A61K39/39 ,  C12P19/12 ,  C12P19/26 ,  C12P19/44 ,  A61K39/00

CPC分类号： C12P19/12 ,  A61K31/739 ,  A61K39/0291 ,  A61K39/099 ,  A61K39/39 ,  C07H11/04 ,  C12P19/26 ,  C12P19/44 ,  A61K2039/55572

摘要： Embodiments of the disclosure provide for unique lipooligosaccharide/lipid A-based mimetics for use as adjuvants. Methods of generating lipooligosaccharide/lipid A-based mimetics are provided that utilize recombinantly engineered bacteria to produce the mimetics, including, for example, addition of one or more particular enzymes such as acyltransferases, deacylases, phosphatases, or glycosyltransferases.

公开(公告)号：US20240287565A1

公开(公告)日：2024-08-29

申请号：US18546949

申请日：2022-02-22

申请人： Chr. Hansen A/S

发明人： Vojislav VOJINOVIC ,  Kim Ib SOERENSEN ,  Laurent GUINOT

IPC分类号： C12P19/12 ,  A23C9/123 ,  C12N1/20 ,  C12N9/12 ,  C12R1/46

CPC分类号： C12P19/12 ,  A23C9/1238 ,  C12N1/20 ,  C12N9/1205 ,  C12R2001/46 ,  C12Y207/01002 ,  C12Y207/01006

摘要： The invention relates to a method of obtaining a composition comprising allolactose by a applying one or more glucose-deficient lactic acid bacteria strains. The invention also relates to the use of said strains for the preparation of a food product comprising allolactose and to the use of said strains for increasing the content of allolactose in a food product. The invention also relates to a food product comprising allolactose and one or more glucose-deficient lactic acid bacteria strains.

公开(公告)号：US20240209405A1

公开(公告)日：2024-06-27

申请号：US18040356

申请日：2021-08-10

申请人： INBIOSE N.V.

发明人： Sofie Aesaert ,  Joeri Beauprez ,  Pieter Coussement ,  Thomas Decoene ,  Nausicaa Lannoo ,  Gert Peters ,  Kristof Vandewalle ,  Annelies Vercauteren

IPC分类号： C12P19/12 ,  C12N1/20 ,  C12N9/10 ,  C12P19/18

CPC分类号： C12P19/12 ,  C12N1/205 ,  C12N9/1051 ,  C12N9/1081 ,  C12P19/18 ,  C12N2500/34 ,  C12Y204/01038 ,  C12Y204/99001 ,  C12Y204/99004 ,  C12Y204/99007

摘要： This disclosure is in the technical field of synthetic biology and metabolic engineering. More particularly, this disclosure is in the technical field of cultivation or fermentation of metabolically engineered cells. This disclosure describes a cell metabolically engineered for production of a mixture of at least three different sialylated oligosaccharides. Furthermore, this disclosure provides a method for the production of a mixture of at least three different sialylated oligosaccharides by a cell as well as the purification of at least one of the sialylated oligosaccharides from the cultivation.

公开(公告)号：US20240200021A1

公开(公告)日：2024-06-20

申请号：US18555663

申请日：2022-04-15

申请人： INBIOSE N.V.

发明人： Joeri Beauprez ,  Pieter Coussement ,  Thomas Decoene

IPC分类号： C12N1/20 ,  C12N1/18 ,  C12N9/10 ,  C12P9/00 ,  C12P19/04 ,  C12P19/12 ,  C12P19/26 ,  C12R1/19

CPC分类号： C12N1/205 ,  C12N1/185 ,  C12N9/1081 ,  C12P9/00 ,  C12P19/04 ,  C12P19/12 ,  C12P19/26 ,  C12N2500/34 ,  C12R2001/19

摘要： The present invention is in the technical field of synthetic biology and metabolic engineering. More particularly, the present invention is in the technical field of metabolically engineered cells of microorganisms and the use of said cells in a fermentation. The present invention describes a metabolically engineered cell of a microorganism and a method by fermentation with said cell for production of a sialylated di- and/or oligosaccharide. The metabolically engineered cell comprises a pathway for production of said sialylated di- and/or oligosaccharide, synthesizes sialic acid, expresses at least one sialyltransferase, preferably is modified in the expression or activity of at least one sialyltransferase, is modified to have a fully or partially knocked out or rendered less functional sialic acid catabolic pathway and is modified for overexpression of an endogenous sialic acid transporter and/or expression, preferably overexpression, of an exogenous, homologous and/or heterologous sialic acid transporter. Furthermore, the present invention provides for purification of said sialylated di- and/or oligosaccharide from the cultivation, preferably fermentation.

公开(公告)号：US11999764B2

公开(公告)日：2024-06-04

申请号：US17828457

申请日：2022-05-31

申请人： Julius-Maximilians-Universitaet Wuerzburg

发明人： Christian Possiel ,  Juergen Seibel

IPC分类号： C07H7/033 ,  C12P19/12 ,  C12P19/18

CPC分类号： C07H7/033 ,  C12P19/12 ,  C12P19/18

摘要： The present invention relates to a new class of sucrose esters and a method for their preparation.

公开(公告)号：US11981947B2

公开(公告)日：2024-05-14

申请号：US17814333

申请日：2022-07-22

申请人： Chr. Hansen HMO GmbH

发明人： Stefan Jennewein

IPC分类号： C12P19/12 ,  C07H1/00 ,  C07H1/06 ,  C12N1/20 ,  C12N15/72 ,  C12P19/00 ,  C12P19/14 ,  C12P19/26 ,  C12R1/19

CPC分类号： C12P19/26 ,  C07H1/00 ,  C07H1/06 ,  C12N1/205 ,  C12N15/72 ,  C12P19/00 ,  C12P19/12 ,  C12P19/14 ,  C12Y302/01 ,  C12Y302/01023 ,  C12N2800/101 ,  C12R2001/19

摘要： The present invention relates to the use of one or more glycosidases in the process for the production and/or purification of a produced desired oligosaccharide. The process is preferably a microbial fermentation process using a host microorganism, which may also comprise nucleic acids expressing sugar catabolic pathway proteins suitable for the degradation of saccharides otherwise hindering the purification of the desired oligosaccharide.

公开(公告)号：US20240084347A1

公开(公告)日：2024-03-14

申请号：US18261793

申请日：2022-01-17

申请人： INBIOSE N.V.

发明人： Joeri Beauprez ,  Sofie De Maeseneire ,  Yatti De Nijs ,  Tom Delmulle

IPC分类号： C12P19/12 ,  C12N9/10 ,  C12P19/18

CPC分类号： C12P19/12 ,  C12N9/1048 ,  C12N9/1051 ,  C12N9/1081 ,  C12N9/1096 ,  C12P19/18 ,  C12Y204/01222 ,  C12Y204/99003 ,  C12Y204/99004 ,  C12Y206/01016

摘要： This disclosure is in the technical field of synthetic biology and metabolic engineering. More particularly, this disclosure is in the technical field of cultivation of metabolically engineered cells. This disclosure describes a method for the production of a glycosylated product derived from UDP-GlcNAc and comprising a di- or oligosaccharide that is composed of at least two different monosaccharide subunits by a cell as well as the separation of the glycosylated product from the cultivation. Furthermore, this disclosure provides a metabolically engineered cell for production of a glycosylated product derived from UDP-GlcNAc and comprising a di- or oligosaccharide that is composed of at least two different monosaccharide subunits. This disclosure also provides a cell excreting a di- or oligosaccharide out of the cell.

公开(公告)号：US20240076704A1

公开(公告)日：2024-03-07

申请号：US17767400

申请日：2020-10-14

申请人： INBIOSE N.V.

发明人： Joeri Beauprez ,  Pieter Coussement ,  Nausicaä Lannoo ,  Gert Peters ,  Kristof Vandewalle ,  Annelies Vercauteren ,  Sofie Aesaert ,  Thomas Decoene

IPC分类号： C12P19/02 ,  C07K14/245 ,  C12N1/20 ,  C12N15/70 ,  C12P19/04 ,  C12P19/12 ,  C12P19/44

CPC分类号： C12P19/02 ,  C07K14/245 ,  C12N1/20 ,  C12N15/70 ,  C12P19/04 ,  C12P19/12 ,  C12P19/44 ,  C12R2001/19

摘要： Described is a method of producing bioproducts by fermentation with a genetically modified cell, as well as to the genetically modified cell used in the method. The cell is genetically modified to produce a bioproduct and is further genetically modified by reducing the expression of at least one endogenous membrane protein encoding gene and/or mutating the expression of the endogenous membrane protein.

公开(公告)号：US20240018560A1

公开(公告)日：2024-01-18

申请号：US18333776

申请日：2023-06-13

申请人： Novozymes, Inc.

发明人： Brett McBrayer ,  Elena Vasenko ,  Tarana Shaghasi

IPC分类号： C12P19/14 ,  C12N1/22 ,  C12N9/42 ,  C12P19/02 ,  C12N9/24 ,  C12P19/12 ,  C12P21/02

CPC分类号： C12P19/14 ,  C12N1/22 ,  C12N9/2445 ,  C12P19/02 ,  C12Y302/01004 ,  C12Y302/01021 ,  C12Y302/01091 ,  C12N9/2437 ,  C12N9/248 ,  C12P19/12 ,  C12Y302/01008 ,  C12N9/2402 ,  C12P21/02 ,  C12Y302/01 ,  Y02P20/52 ,  C12Y302/01037 ,  C12P2203/00

摘要： The present invention relates to enzyme compositions for high temperature saccharification of cellulosic material and to uses thereof.

公开(公告)号：US20230399669A1

公开(公告)日：2023-12-14

申请号：US18207453

申请日：2023-06-08

申请人： Chr. Hansen HMO GmbH

发明人： Stefan Jennewein ,  Dirk Wartenberg ,  Katja Parschat

IPC分类号： C12P19/12 ,  C12N1/20 ,  C12N9/10 ,  C12N9/12 ,  C12N9/16 ,  C12N9/90 ,  C12P19/04 ,  C12P19/18

CPC分类号： C12P19/12 ,  C12N1/20 ,  C12N9/1051 ,  C12N9/1205 ,  C12N9/16 ,  C12N9/90 ,  C12P19/04 ,  C12P19/18 ,  C12N2500/34 ,  C12Y204/01038 ,  C12Y207/01004 ,  C12Y207/01011 ,  C12Y207/01056 ,  C12Y207/07009 ,  C12Y301/03011 ,  C12Y501/03002 ,  C12Y504/02002

摘要： Disclosed are genetically engineered microbial cells for the production of oligosaccharides comprising a galactose-β1,4-glucose moiety at their reducing end, wherein said microbial cells are able to produce said oligosaccharides in the absence of exogenously added lactose, and a method of producing said oligosaccharides using said microbial cells.