公开(公告)号：US20240277834A1

公开(公告)日：2024-08-22

申请号：US18417362

申请日：2024-01-19

申请人： ASTRAZENECA AB

发明人： Albert George THOM ,  Joseph Richard FRANCICA ,  Wade Stanton BLAIR ,  Jason Paul LALIBERTE ,  Julie Ann DOUTHWAITE ,  Philip A. NEWTON ,  Gareth John GRIFFIN ,  Colin Harold HARDMAN ,  Yueh-Ming LOO

IPC分类号： A61K39/215 ,  A61P31/14 ,  A61P37/04 ,  C07K14/47 ,  C12N9/10 ,  C12N9/12 ,  C12N9/42 ,  A61K39/00

CPC分类号： A61K39/215 ,  A61P31/14 ,  A61P37/04 ,  C07K14/47 ,  C12N9/1025 ,  C12N9/1096 ,  C12N9/12 ,  C12N9/2442 ,  C12Y203/03001 ,  C12Y206/01001 ,  C12Y207/11011 ,  C12Y302/01014 ,  A61K2039/6031 ,  A61K2039/6056 ,  C07K2319/30

摘要： The present disclosure relates to a nucleic acid molecule comprising 5′-UTR and/or 3′-UTR sequences that yield high translation levels. Aspects of the disclosure further relate to nucleic acid molecules suitable for use as a vaccine in the treatment and prevention of infectious diseases, including those caused by a coronavirus, compositions comprising said nucleic acid molecules and methods of treating or preventing infectious diseases.

公开(公告)号：US20240158737A1

公开(公告)日：2024-05-16

申请号：US18420028

申请日：2024-01-23

申请人： JIANGNAN UNIVERSITY

发明人： Zhiming RAO ,  Yanan HAO ,  Meijuan XU ,  Xuewei PAN ,  Jiajia YOU ,  Taowei YANG ,  Xian ZHANG ,  Mingling SHAO

IPC分类号： C12N1/20 ,  C12N9/10 ,  C12N9/88 ,  C12N15/70 ,  C12P13/08

CPC分类号： C12N1/205 ,  C12N9/1096 ,  C12N9/88 ,  C12N15/70 ,  C12P13/08 ,  C12Y206/01042 ,  C12Y402/01009 ,  C12Y402/01012 ,  C12R2001/19

摘要： The invention provides an Escherichia coli for synthesizing L-valine, a construction method and use thereof. The Escherichia coli of the invention is designated as Escherichia coli W3110 and was deposited in China Center for Type Culture Collection (Address: Bayi Road, Wuchang District, Wuhan City, Hubei Province) under the Accession No. CCTCC M 2022293 on Mar. 18, 2022. The recombinant Escherichia coli takes Escherichia coli as a starting strain, and a transcription regulation factor is overexpressed to obtain a recombinant Escherichia coli. The recombinant Escherichia coli for synthesizing L-valine of the invention is fermented in a 5 L fermentor with trace dissolved oxygen to test strains, the yield of L-valine reaches 112 g/L, and the OD of the bacterium is 104.

公开(公告)号：US20240141397A1

公开(公告)日：2024-05-02

申请号：US18242912

申请日：2023-09-06

申请人： Genomatica, Inc.

发明人： Robin E. Osterhout ,  Priti Pharkya

IPC分类号： C12P17/10 ,  C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C12N9/16 ,  C12N15/52 ,  C12P7/18 ,  C12P7/40 ,  C12P7/44 ,  C12P13/00 ,  C12P17/08

CPC分类号： C12P17/10 ,  C12N9/0004 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/0016 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/13 ,  C12N9/16 ,  C12N9/93 ,  C12N15/52 ,  C12P7/18 ,  C12P7/40 ,  C12P7/44 ,  C12P13/001 ,  C12P13/005 ,  C12P17/08 ,  C12Y101/01027 ,  C12Y101/0103 ,  C12Y101/01035 ,  C12Y101/01037 ,  C12Y101/01172 ,  C12Y103/01031 ,  C12Y104/01009 ,  C12Y203/01174 ,  C12Y206/01019 ,  C12Y208/03006 ,  C12Y301/02004 ,  C12Y603/03004

摘要： Provided herein are non-naturally occurring microbial organisms having biosynthetic pathways for production of target products and one or more genetic modifications that reduce a byproduct of the biosynthetic pathway. Compositions of target products from such cells and methods of using such cells are provided.

公开(公告)号：US20240124853A1

公开(公告)日：2024-04-18

申请号：US18264091

申请日：2021-07-06

申请人： ASYMCHEM LABORATORIES (TIANJIN) CO., LTD

发明人： Hao Hong ,  Gage James ,  Yi Xiao ,  Na Zhang ,  Xuecheng Jiao ,  Yulei Ma ,  Huiyan Mou ,  Zujian Wang ,  Kaihua Sun ,  Xiang Li ,  Tong Zhao ,  Shan Cao

IPC分类号： C12N9/10 ,  C12P13/00 ,  C12P17/12 ,  C12P17/14

CPC分类号： C12N9/1096 ,  C12P13/001 ,  C12P17/12 ,  C12P17/14 ,  C12Y206/01

摘要： Provided are transaminase mutants and uses thereof. The transaminase mutant is obtained by one or more amino acid mutations occurring in SEQ ID NO: 2 or is a mutant with a conserved amino acid mutation obtained by taking the sequence SEQ ID NO: 1 of a wild-type CvTA transaminase as a reference. Compared with wild-type transaminases, the catalytic activity of the mutant is improved to different degrees, so that the production efficiency of chiral amine compound synthesis may be improved.

公开(公告)号：US11939583B2

公开(公告)日：2024-03-26

申请号：US17047974

申请日：2019-04-15

申请人： Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.

发明人： Lennart Schada Von Borzyskowski ,  Tobias Jürgen Erb ,  Jan Zarzycki

IPC分类号： C12N15/52 ,  C12N1/21 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C12N9/16 ,  C12N9/88 ,  C12N15/74

CPC分类号： C12N15/52 ,  C12N9/0006 ,  C12N9/0022 ,  C12N9/1096 ,  C12N9/16 ,  C12N9/88 ,  C12N15/74 ,  C12Y101/01026 ,  C12Y104/03016 ,  C12Y206/01001 ,  C12Y301/03018 ,  C12Y401/03014 ,  C12Y403/0102

摘要： The present invention relates to autotrophic microorganisms with altered photorespiration and improved CO2 fixation as well as a method of producing said autotrophic microorganisms. Particularly, the autotrophic microorganisms show an improved growth rate, productivity and energy conversion efficiency.

公开(公告)号：US20240002816A1

公开(公告)日：2024-01-04

申请号：US18459797

申请日：2023-09-01

申请人： Codexis, Inc.

发明人： Scott J. Novick ,  Nikki Dellas

IPC分类号： C12N9/10

CPC分类号： C12N9/1096

摘要： The present disclosure provides engineered transaminase polypeptides useful for the synthesis of chiral amine compounds under industrially relevant conditions. The disclosure also provides polynucleotides encoding the engineered transaminase polypeptides, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases for the production of chiral amine compounds.

公开(公告)号：US20230392173A1

公开(公告)日：2023-12-07

申请号：US18021788

申请日：2021-08-19

申请人： Zymergen Inc.

发明人： Zhangying Hao ,  Shuchi Desai ,  Steven M. Edgar ,  Joseph Gallagher ,  James Chandler ,  Eyal Akiva ,  Rebecca Davidson ,  Matthew Davis

IPC分类号： C12P13/00 ,  C12N15/81 ,  C12N9/10 ,  C12N9/90 ,  C12N9/02 ,  C12N9/88 ,  C12N1/16

CPC分类号： C12P13/001 ,  C12N15/815 ,  C12N9/1096 ,  C12N9/90 ,  C12N9/001 ,  C12N9/88 ,  C12N1/16 ,  C12Y206/01085 ,  C12Y504/99 ,  C12Y103/01 ,  C12Y401/01

摘要： The present disclosure describes the engineering of microbial cells for fermentative production of 4-APEA and related products and provides novel engineered microbial cells and cultures, as well as related 4-APEA production methods.

公开(公告)号：US11814660B2

公开(公告)日：2023-11-14

申请号：US17120586

申请日：2020-12-14

申请人： Genomatica, Inc.

发明人： Stephen B. Del Cardayre ,  Louis G. Hom

IPC分类号： C12P13/00 ,  C12N9/02 ,  C12N9/10 ,  C12N9/16 ,  C12N9/00 ,  C12N9/06

CPC分类号： C12P13/001 ,  C12N9/0008 ,  C12N9/0014 ,  C12N9/1096 ,  C12N9/16 ,  C12N9/93 ,  C12Y102/01 ,  C12Y102/01042 ,  C12Y104/09001 ,  C12Y206/01019 ,  C12Y206/01082 ,  C12Y301/02 ,  C12Y602/01003

摘要： The disclosure relates to recombinant microorganisms for the production of fatty amines and derivatives thereof. Further contemplated are cultured recombinant host cells as well as methods of producing fatty amines by employing these host cells.

公开(公告)号：US20230348865A1

公开(公告)日：2023-11-02

申请号：US17996921

申请日：2021-04-23

申请人： Genomatica, Inc.

发明人： Amit Mahendra SHAH ,  Deqiang ZHANG ,  Joseph Roy WARNER ,  Benjamin Matthew GRIFFIN ,  Russell Scott KOMOR ,  Harish NAGARAJAN ,  Sankha GHATAK ,  Toby Howard RICHARDSON

IPC分类号： C12N9/02 ,  C12P13/00 ,  C12P7/04 ,  C12N15/52 ,  C12N9/10 ,  C12N9/88

CPC分类号： C12N9/0008 ,  C12Y102/0103 ,  C12P13/001 ,  C12P7/04 ,  C12N15/52 ,  C12N9/1029 ,  C12Y203/01174 ,  C12Y102/01003 ,  C12N9/88 ,  C12Y402/01017 ,  C12N9/1096

摘要： The invention provides an engineered carboxylic acid reductase (CAR) enzyme, a nucleic acid encoding the CAR enzyme, and a non-naturally occurring microbial organism comprising an exogenous nucleic acid encoding the CAR, an engineered transaminase (TA) enzyme, and/or a hexamethylenediamine (HMD) transaminase (TA2) enzyme. The invention provides a non-naturally occurring microbial organism that has a 1,6-hexanediol (HDO) pathway with a HDO pathway enzyme expressed in sufficient amounts to produce 6 aminocaproate semi aldehyde, HDO, or both. The invention further provides a non-naturally occurring microbial organism that has an HMD pathway with a HMD pathway enzyme expressed in sufficient amounts to produce 6-aminocaproate semialdehyde, HMD, or both. The invention additionally provides bioderived HMD, 6-aminocaproate semialdehyde, and/or HDO and methods for producing bioderived HMD, 6-aminocaproate semialdehyde, and/or HDO.

公开(公告)号：US20230332115A1

公开(公告)日：2023-10-19

申请号：US18344690

申请日：2023-06-29

申请人： Codexis, Inc.

发明人： Weng Lin Tang ,  Helen Hsieh ,  Son Pham ,  Derek Smith ,  Steven J. Collier

IPC分类号： C12N9/10 ,  C12P17/12 ,  C12P17/18 ,  C12P13/00 ,  C12P33/00 ,  C12P15/00

CPC分类号： C12N9/1096 ,  C12P17/12 ,  C12P17/188 ,  C12P13/001 ,  C12P33/00 ,  C12Y206/01 ,  C12P15/00

摘要： The present disclosure provides engineered transaminase polypeptides for the production of amines, polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases to prepare compounds useful in the production of active pharmaceutical agents.