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    Genetically Modified Cell and Process for Use of Said Cell
    3.
    发明申请
    Genetically Modified Cell and Process for Use of Said Cell 有权
    遗传修饰的细胞和所述细胞的使用方法

    公开(公告)号：US20160257978A1

    公开(公告)日：2016-09-08

    申请号：US15059857

    申请日：2016-03-03

    申请人： Purac Biochem B.V.

    发明人： Nick Johannes Petrus Wierckx ,  Tom Daniel Elink Schuurman ,  Sipko Maarten Kuijper ,  Harald Johan Ruijssenaars

    IPC分类号： C12P17/04 ,  C12N9/02 ,  C07K14/195

    CPC分类号： C12P17/04 ,  C07K14/195 ,  C12N9/0004 ,  C12N9/0008 ,  C12Y102/01

    摘要： The present invention relates to the field of biotransformation of furanic compounds. More particular the present invention relates to novel genetically modified cells with improved characteristics for biocatalytic transformation of furanic compounds and a vector suitable for the genetic modification of a host cell. Further aspects of the invention are aimed at processes for biotransformation of 5-(hydroxymethyl)furan-2-carboxylic acid (HMF-acid) and its precursors with the use of the cell according to the invention.

    摘要翻译： 本发明涉及呋喃化合物的生物转化领域。 更具体地，本发明涉及具有改进的呋喃化合物的生物催化转化特性和适用于宿主细胞的遗传修饰的载体的新型遗传修饰细胞。 本发明的其它方面的目的在于使用本发明的细胞生物转化5-（羟甲基）呋喃-2-羧酸（HMF-酸）及其前体的方法。

    ORGANISMS FOR THE PRODUCTION OF 1,3-BUTANEDIOL
    6.
    发明申请
    ORGANISMS FOR THE PRODUCTION OF 1,3-BUTANEDIOL 审中-公开
    生产1,3-丁二醇的有机体

    公开(公告)号：US20160053286A1

    公开(公告)日：2016-02-25

    申请号：US14673600

    申请日：2015-03-30

    申请人： Genomatica, Inc.

    发明人： Anthony P. Burgard ,  Mark J. Burk ,  Robin E. Osterhout ,  Priti Pharkya

    IPC分类号： C12P7/18 ,  C07C29/80 ,  C12N9/04 ,  C12N9/02 ,  C12N9/10 ,  C12N9/88

    CPC分类号： C12P7/18 ,  C07C29/80 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/88 ,  C12N15/52 ,  C12Y101/01 ,  C12Y101/01157 ,  C12Y102/01 ,  C12Y203/01 ,  C12Y206/01 ,  C12Y401/01 ,  C12Y402/01 ,  C12Y403/01

    摘要： A non-naturally occurring microbial organism includes a microbial organism having a 1,3-butanediol (1,3-BDO) pathway having at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO. The pathway includes an enzyme selected from a 2-amino-4-ketopentanoate (AKP) thiolase, an AKP dehydrogenase, a 2-amino-4-hydroxypentanoate aminotransferase, a 2-amino-4-hydroxypentanoate oxidoreductase (deaminating), a 2-oxo-4-hydroxypentanoate decarboxylase, a 3-hydroxybutyraldehyde reductase, an AKP aminotransferase, an AKP oxidoreductase (deaminating), a 2,4-dioxopentanoate decarboxylase, a 3-oxobutyraldehyde reductase (ketone reducing), a 3-oxobutyraldehyde reductase (aldehyde reducing), a 4-hydroxy-2-butanone reductase, an AKP decarboxylase, a 4-aminobutan-2-one aminotransferase, a 4-aminobutan-2-one oxidoreductase (deaminating), a 4-aminobutan-2-one ammonia-lyase, a butenone hydratase, an AKP ammonia-lyase, an acetylacrylate decarboxylase, an acetoacetyl-CoA reductase (CoA-dependent, aldehyde forming), an acetoacetyl-CoA reductase (CoA-dependent, alcohol forming), an acetoacetyl-CoA reductase (ketone reducing), a 3-hydroxybutyryl-CoA reductase (aldehyde forming), a 3-hydroxybutyryl-CoA reductase (alcohol forming), a 4-hydroxybutyryl-CoA dehydratase, and a crotonase. A method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO.

    摘要翻译： 非天然存在的微生物生物体包括具有1,3-丁二醇（1,3-BDO）途径的微生物生物，其具有至少一种编码1,3-BDO途径酶的外源核酸，所述外源核酸以足够的量表达，以产生1 ，3-BDO。 该途径包括选自2-氨基-4-酮戊酸酯（AKP）硫解酶，AKP脱氢酶，2-氨基-4-羟基戊酸转氨酶，2-氨基-4-羟基戊酸酯氧化还原酶（脱氨基），2- 4-羟基戊酸脱羧酶，3-羟基丁醛还原酶，AKP氨基转移酶，AKP氧化还原酶（脱氨），2,4-二氧代戊酸脱羧酶，3-氧代丁醛还原酶（还原酮），3-氧代丁醛还原酶 ），4-羟基-2-丁酮还原酶，AKP脱羧酶，4-氨基丁-2-酮氨基转移酶，4-氨基丁-2-酮氧化还原酶（脱氨基），4-氨基丁-2-酮氨裂解酶 乙酰乙酰辅酶A还原酶（CoA依赖性醛形成），乙酰乙酰辅酶A还原酶（CoA依赖性醇形成），乙酰乙酰辅酶A还原酶（酮），乙酰乙酰辅酶A还原酶 还原），3-羟基丁酰辅酶A还原酶（醛形成），3-羟基 Ryl-CoA还原酶（醇形成），4-羟基丁酰辅酶A脱水酶和巴豆酸酶。 一种生产1,3-BDO的方法包括在条件和足够的时间内培养这些微生物生产1,3-BDO。

    MICROORGANISMS FOR THE PRODUCTION OF METHACRYLIC ACID
    9.
    发明申请
    MICROORGANISMS FOR THE PRODUCTION OF METHACRYLIC ACID 有权
    用于生产甲基丙烯酸的微生物

    公开(公告)号：US20120276604A1

    公开(公告)日：2012-11-01

    申请号：US13531377

    申请日：2012-06-22

    申请人： Anthony P. Burgard ,  Mark J. Burk ,  Robin E. Osterhout ,  Priti Pharkya

    发明人： Anthony P. Burgard ,  Mark J. Burk ,  Robin E. Osterhout ,  Priti Pharkya

    IPC分类号： C12P7/42 ,  C12N1/21

    CPC分类号： C12P7/42 ,  C12N9/0008 ,  C12N9/1096 ,  C12N9/88 ,  C12N9/90 ,  C12P7/40 ,  C12Y102/01 ,  C12Y206/01022 ,  C12Y403/01 ,  C12Y504/99002

    摘要： The invention provides a non-naturally occurring microbial organism having a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The invention additionally provides a method for producing 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid. The method can include culturing a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid producing microbial organism expressing at least one exogenous nucleic acid encoding a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway enzyme in a sufficient amount and culturing under conditions and for a sufficient period of time to produce 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid.

    摘要翻译： 本发明提供了具有2-羟基异丁酸，3-羟基异丁酸或甲基丙烯酸途径的非天然存在的微生物生物。 微生物生物体含有编码2-羟基异丁酸，3-羟基异丁酸或甲基丙烯酸途径中的酶的至少一种外源核酸。 本发明还提供了2-羟基异丁酸，3-羟基异丁酸或甲基丙烯酸的制备方法。 该方法可以包括培养足够量的表达至少一种编码2-羟基异丁酸，3-羟基异丁酸或甲基丙烯酸途径酶的外源核酸的2-羟基异丁酸，3-羟基异丁酸或产生甲基丙烯酸的微生物，并培养 在条件下和足够的时间内产生2-羟基异丁酸，3-羟基异丁酸或甲基丙烯酸。