知搜-全球专利检索

  1. Login
  2. Sign Up

Search Results

58 records (took 0.076 seconds)

    Selection of host cells expressing protein at high levels
    6.
    发明申请
    Selection of host cells expressing protein at high levels 审中-公开
    选择高水平表达蛋白质的宿主细胞

    公开(公告)号：US20060195935A1

    公开(公告)日：2006-08-31

    申请号：US11416490

    申请日：2006-05-02

    Applicant: Arie Otte ,  Henricus Van Blokland ,  Theodorus Kwaks ,  Richard Bernardus Sewalt

    Inventor： Arie Otte ,  Henricus Van Blokland ,  Theodorus Kwaks ,  Richard Bernardus Sewalt

    IPC: A01H1/00 ,  C07H21/04 ,  C12N15/82 ,  C12N5/04

    CPC classification number: C07K14/505 ,  C12N9/1205 ,  C12N15/67 ,  C12N15/85 ,  C12N2830/46 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/206 ,  C12N2840/50 ,  C12Y207/01095

    Abstract: The invention provides a DNA molecule comprising an open reading frame sequence that encodes a selectable marker polypeptide, wherein said DNA molecule in the coding strand comprises a translation start sequence for the selectable marker polypeptide having a GTG startcodon or a TTG startcodon, and wherein the open reading frame sequence that encodes the selectable marker protein has been mutated to replace at least half of its CpG dinucleotides as compared to the native open reading frame sequence that encodes the selectable marker protein. The invention further provides such DNA molecules wherein the open reading frame sequence that encodes a selectable marker polypeptide is part of a multicistronic transcription unit that further comprises an open reading frame sequence encoding a polypeptide of interest. The invention also provides methods for obtaining host cells expressing a polypeptide of interest, said host cells comprising the DNA molecules of the invention. The invention further provides the production of polypeptides of interest, comprising culturing host cells comprising the DNA molecules according to the invention.

    Abstract translation: 本发明提供了包含编码选择性标记多肽的开放阅读框序列的DNA分子，其中编码链中的所述DNA分子包含具有GTG起始密码子或TTG起始密码子的可选择标记多肽的翻译起始序列，并且其中所述开放 与编码可选择标记蛋白的天然开放阅读框序列相比，编码可选择标记蛋白的阅读框序列已被突变以替代其至少一半的CpG二核苷酸。 本发明还提供了这样的DNA分子，其中编码可选择标记多肽的开放阅读框序列是多顺反子转录单位的一部分，其进一步包含编码目的多肽的开放阅读框序列。 本发明还提供了获得表达目的多肽的宿主细胞的方法，所述宿主细胞包含本发明的DNA分子。 本发明进一步提供感兴趣的多肽的生产，包括培养包含根据本发明的DNA分子的宿主细胞。

Patent Agency Ranking