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公开(公告)号：US20120309918A1
公开(公告)日：2012-12-06
申请号：US13393496
申请日：2010-09-02
申请人： Harald Johan Ruijssenaars , Nick Johannes Petrus Wierckx , Frank Wouter Koopman , Adrianus Johannes Jozef Straathof , Johannes Hendrik De Winde
发明人： Harald Johan Ruijssenaars , Nick Johannes Petrus Wierckx , Frank Wouter Koopman , Adrianus Johannes Jozef Straathof , Johannes Hendrik De Winde
CPC分类号： C12P17/04 , C08F124/00 , C12N9/0006 , C12N9/0008 , Y02P20/52
摘要： The invention relates to a polypeptide having oxidoreductase activity which comprises the amino acid sequence set out in SEQ ID NO: 3 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 4, or a variant polypeptide thereof having 45% or more sequence identity with the sequence of SEQ ID NO: 3. The invention also relates to a process for the production of 2,5-furan-dicarboxylic acid (FDCA) or production of 5-hydroxymethyl-2-furancarboxylic acid (HMF acid).
摘要翻译： 本发明涉及具有氧化还原酶活性的多肽，其包含SEQ ID NO：3所示的氨基酸序列或由SEQ ID NO：4的核苷酸序列编码的氨基酸序列，或其变体多肽具有45％以上 序列同一性。本发明还涉及生产2,5-呋喃二羧酸（FDCA）或生产5-羟甲基-2-呋喃甲酸（HMF酸）的方法。
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公开(公告)号：US20110086395A1
公开(公告)日：2011-04-14
申请号：US12874913
申请日：2010-09-02
申请人： Frank Wouter KOOPMAN , Harald Johan Ruijssenaars , Nick Johannes Petrus Wierckx , Johannes Hendrik De Winde
发明人： Frank Wouter KOOPMAN , Harald Johan Ruijssenaars , Nick Johannes Petrus Wierckx , Johannes Hendrik De Winde
IPC分类号： C12P7/06 , C12N1/20 , C12N9/06 , C07H21/04 , C07K14/195 , C12N9/88 , C12N9/04 , C12N9/02 , C12N9/00 , C12N9/16 , C12N15/63 , C12N5/10 , C12N1/21 , C12N1/19 , C12N1/15 , C12P19/32
CPC分类号： C12R1/01 , C07K14/195 , C12N9/0008 , C12N9/001 , C12N9/16 , C12N9/88 , C12N9/93 , C12N15/78 , C12P19/32 , Y02E50/17 , Y02E50/343
摘要： An isolated microorganism is provided which is Cupriavidus basilensis strain HMF14 Deposit number DSM 22875, and its use in a process for the in-situ detoxification of lignocelluloses hydrolysate.
摘要翻译： 提供了一种分离的微生物，其是巴西利亚瓦利酵母菌株HMF14保藏号DSM 22875，并且其用于木质纤维素水解产物的原位解毒的方法中。
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公开(公告)号：US09309546B2
公开(公告)日：2016-04-12
申请号：US14003386
申请日：2012-03-07
申请人： Nick Johannes Petrus Wierckx , Tom Daniel Elink Schuurman , Sipko Maarten Kuijper , Harald Johan Ruijssenaars
发明人： Nick Johannes Petrus Wierckx , Tom Daniel Elink Schuurman , Sipko Maarten Kuijper , Harald Johan Ruijssenaars
IPC分类号： C07K14/195 , C12P17/04 , C12N9/02
CPC分类号： C12P17/04 , C07K14/195 , C12N9/0004 , C12N9/0008 , C12Y102/01
摘要： The present invention relates to the field of biotransformation of furanic compounds. More particular the present invention relates to novel genetically modified cells with improved characteristics for biocatalytic transformation of furanic compounds and a vector suitable for the genetic modification of a host cell. Further aspects of the invention are aimed at processes for biotransformation of 5-(hydroxymethyl)furan-2-carboxylic acid (HMF-acid) and its precursors with the use of the cell according to the invention.
摘要翻译： 本发明涉及呋喃化合物的生物转化领域。 更具体地，本发明涉及具有改进的呋喃化合物的生物催化转化特性和适用于宿主细胞的遗传修饰的载体的新型遗传修饰细胞。 本发明的其它方面的目的在于使用本发明的细胞生物转化5-（羟甲基）呋喃-2-羧酸（HMF-酸）及其前体的方法。
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公开(公告)号：US09045787B2
公开(公告)日：2015-06-02
申请号：US13393496
申请日：2010-09-02
申请人： Harald Johan Ruijssenaars , Nick Johannes Petrus Wierckx , Frank Wouter Koopman , Adrianus Johannes Jozef Straathof , Johannes Hendrik De Winde
发明人： Harald Johan Ruijssenaars , Nick Johannes Petrus Wierckx , Frank Wouter Koopman , Adrianus Johannes Jozef Straathof , Johannes Hendrik De Winde
CPC分类号： C12P17/04 , C08F124/00 , C12N9/0006 , C12N9/0008 , Y02P20/52
摘要： The invention relates to a polypeptide having oxidoreductase activity which comprises the amino acid sequence set out in SEQ ID NO: 3 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 4, or a variant polypeptide thereof having 45% or more sequence identity with the sequence of SEQ ID NO: 3. The invention also relates to a process for the production of 2,5-furan-dicarboxylic acid (FDCA) or production of 5-hydroxymethyl-2-furancarboxylic acid (HMF acid).
摘要翻译： 本发明涉及具有氧化还原酶活性的多肽，其包含SEQ ID NO：3所示的氨基酸序列或由SEQ ID NO：4的核苷酸序列编码的氨基酸序列，或其变体多肽具有45％以上 序列同一性。本发明还涉及生产2,5-呋喃二羧酸（FDCA）或生产5-羟甲基-2-呋喃甲酸（HMF酸）的方法。
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