公开(公告)号：US20240318209A1

公开(公告)日：2024-09-26

申请号：US18627530

申请日：2024-04-05

申请人： Beijing University of Chemical Technology

发明人： Tianwei Tan ,  Yingyue Yu ,  Haowen Zhu ,  Jianyu Long

IPC分类号： C12P7/22 ,  C12N1/20 ,  C12N9/02 ,  C12N9/10 ,  C12N9/12 ,  C12N9/14 ,  C12R1/15

CPC分类号： C12P7/22 ,  C12N1/205 ,  C12N9/0008 ,  C12N9/1007 ,  C12N9/1022 ,  C12N9/1029 ,  C12N9/1085 ,  C12N9/1288 ,  C12N9/14 ,  C12R2001/15 ,  C12Y102/99006 ,  C12Y201/01014 ,  C12Y202/01001 ,  C12Y203/01031 ,  C12Y205/01006 ,  C12Y205/01054 ,  C12Y207/08007 ,  C12Y303/01001

摘要： The present invention discloses a genetically engineered bacterium using glucose as a substrate for de novo synthesis of vanillin and an application thereof, which belongs to the technical field of gene recombination and metabolic engineering. The genetically engineered bacterium using the glucose as the substrate for de novo synthesis of vanillin disclosed by the present invention is recombinant Corynebacterium glutamicum modified by chassis microorganisms and including a vanillin synthesis module and a methyl cyclic regeneration module. The genetically engineered bacteria constructed by the present invention are safe and non-toxic, can use the glucose for de novo synthesis of natural vanillin, and is low in production cost, high in yield, and promising in application prospect.

公开(公告)号：US20180237792A1

公开(公告)日：2018-08-23

申请号：US15902697

申请日：2018-02-22

申请人： The University of Massachusetts

发明人： Om Parkash Dhankher ,  Sudesh Chhikara

IPC分类号： C12N15/82 ,  C12N9/10 ,  C12N9/12 ,  C12N9/04

CPC分类号： C12N15/8247 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/1288 ,  C12N15/8218 ,  C12N15/8261 ,  C12N2830/65 ,  C12Y101/01008 ,  C12Y203/0102 ,  C12Y203/01022 ,  C12Y207/08002

摘要： Compositions and methods for producing plants with enhanced oil content and higher seed yield are disclosed. The transgenic plant comprises a polynucleotide encoding a monoacylglycerol O-acyltransferase 1 (MGAT1) operatively linked to a plant-expressible promoter; a polynucleotide encoding a phosphatidylcholine diacylglycerol cholinephosphotransferase 1 (PDCT1) operatively linked to a plant-expressible promoter; a polynucleotide encoding a suppressor of expression of Sugar Dependent 1 (SPD1) operatively linked to a plant-expressible promoter; a polynucleotide encoding a diacylglyerol acyltransferase (DGAT1) operatively linked to a plant-expressible promoter and a polynucleotide encoding a glycerol-3-phosphate dehydrogenase (GPD1) operatively linked to a plant-expressible promoter; or a combination thereof.

公开(公告)号：US09994828B2

公开(公告)日：2018-06-12

申请号：US14555326

申请日：2014-11-26

申请人： Dow AgroSciences LLC ,  DSM IP Assets B.V.

发明人： Terence A. Walsh ,  Daniel J. Gachotte ,  Cory M. Larsen ,  Scott Bevan ,  P. Ann Owens-Merlo ,  James G. Metz ,  Ross Zirkle

IPC分类号： C12N9/10 ,  C12N15/82 ,  A23D9/00 ,  C11B1/00 ,  C11B1/04 ,  C11B1/10 ,  A23K20/158 ,  C12N9/12

CPC分类号： C12N9/1029 ,  A23D9/00 ,  A23K20/158 ,  C11B1/00 ,  C11B1/04 ,  C11B1/10 ,  C12N9/1288 ,  C12N15/82 ,  C12N15/8247 ,  C12Y203/01085

摘要： This disclosure concerns recombinant host organisms genetically modified with a polyunsaturated fatty acid (PUFA) synthase system and one or more accessory proteins that allow for and/or improve the production of PUFAs in the host organism. The disclosure also concerns methods of making and using such organisms as well as products obtained from such organisms.

公开(公告)号：US09873886B2

公开(公告)日：2018-01-23

申请号：US14409711

申请日：2013-07-04

申请人： Bayer CropScience NV

发明人： Peter Denolf ,  Michel Van Thournout ,  Stephane Bourot

IPC分类号： A01H5/10 ,  C12N15/82 ,  A01H1/04 ,  C12Q1/68 ,  C12N9/12 ,  C11B1/10 ,  C11C3/00 ,  C11B1/00 ,  C07K14/415 ,  A23K10/30

CPC分类号： C12N15/8247 ,  A01H1/04 ,  A01H5/10 ,  A23K10/30 ,  C07K14/415 ,  C11B1/00 ,  C11B1/10 ,  C11C3/00 ,  C11C3/003 ,  C12N9/1288 ,  C12N15/8218 ,  C12Q1/6895 ,  C12Y207/08002

摘要： The present invention relates to Brassica juncea ROD1 nucleic acid sequences and proteins and the use thereof to create plants with increased levels of C18:1 and reduced levels of saturated fatty acids in the seeds.

公开(公告)号：US20170252418A1

公开(公告)日：2017-09-07

申请号：US15510933

申请日：2015-09-16

申请人： HUMBOLDT-UNIVERSITÄT ZU BERLIN ,  UNIVERSITEIT UTRECHT HOLDING B.V.

发明人： Nishith GUPTA ,  Ruben ARROYO-OLARTE ,  Richard LUCIUS ,  Jos BROUWERS ,  J. Bernd HELMS ,  Ildiko DUNAY

IPC分类号： A61K39/012 ,  C12N1/36 ,  C12N1/10 ,  C07F9/10 ,  C12N9/12

CPC分类号： A61K39/012 ,  A61K2039/522 ,  A61K2039/552 ,  C07F9/106 ,  C12N1/10 ,  C12N1/36 ,  C12N9/1288

摘要： A genetically modified coccidian parasites wherein expression of phosphatidylthreonine synthase (PTS) is disrupted, a polynucleotide including a nucleotide sequence encoding a phosphatidylthreonine synthase (PTS) enzyme, which catalyzes the production of a lipid, phosphatidylthreonine (PtdThr). PtdThr is an exclusive, major and physiologically important lipid in selected coccidian parasites, which is required for a normal growth and virulence of coccidian parasites. Coccidian parasites, having the expression of PTS disrupted as described herein, are useful as vaccines. The phosphatidylthreonine synthase enzyme and the nucleotide encoding sequences thereof as well as the phosphatidylthreonine phospholipid can find use in diagnostic methods and diagnostic kits or in vaccine and drug development applications.

公开(公告)号：US09580731B2

公开(公告)日：2017-02-28

申请号：US14138971

申请日：2013-12-23

申请人： INVISTA North America S.á r.l.

发明人： Adriana Leonora Botes ,  Alex Van Eck Conradie ,  Changlin Chen ,  Paul S. Pearlman

IPC分类号： C12P13/00 ,  C12P7/18 ,  C12P7/42 ,  C12P7/44 ,  C12N15/52

CPC分类号： C12N15/52 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1025 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/1288 ,  C12N9/16 ,  C12N9/80 ,  C12N9/88 ,  C12N9/93 ,  C12P7/18 ,  C12P7/24 ,  C12P7/42 ,  C12P7/44 ,  C12P13/001 ,  C12P13/005 ,  C12Y101/01 ,  C12Y101/01085 ,  C12Y101/01087 ,  C12Y101/01258 ,  C12Y101/01286 ,  C12Y102/01 ,  C12Y102/01003 ,  C12Y102/0101 ,  C12Y102/99006 ,  C12Y203/01032 ,  C12Y203/03013 ,  C12Y203/03014 ,  C12Y206/01 ,  C12Y207/08 ,  C12Y208/03012 ,  C12Y301/02 ,  C12Y305/01062 ,  C12Y401/01043 ,  C12Y402/01033 ,  C12Y402/01036 ,  C12Y402/01114 ,  C12Y602/01005

摘要： This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming one or two terminal functional groups, each comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the C1 elongation enzymes or homolog associated with coenzyme B biosynthesis.

摘要翻译： 本文件描述了通过在C7脂肪族中形成一个或两个由羧基，胺或羟基组成的末端官能团，生成庚二酸，7-氨基庚酸，7-羟基庚酸，七亚甲基二胺或1,7-庚二醇的生化途径 骨架底物。 本文描述的这些途径，代谢工程和培养策略依赖于C1延长酶或与辅酶B生物合成相关的同源物。

公开(公告)号：US09528127B2

公开(公告)日：2016-12-27

申请号：US14682354

申请日：2015-04-09

申请人： Joule Unlimited Technologies, Inc.

发明人： Frank Anthony Skraly ,  Ning Li

IPC分类号： C12N1/20 ,  C12N15/00 ,  C12N9/12 ,  C12N9/88 ,  C12N9/16 ,  C12N9/06 ,  C12P5/00 ,  C07H21/04 ,  C12P5/02 ,  C12N15/63 ,  C12N15/74 ,  C12N9/02 ,  C07C9/15 ,  C10L1/08

CPC分类号： C12P5/02 ,  C07C9/15 ,  C10L1/08 ,  C10L2200/04 ,  C10L2270/026 ,  C10L2270/04 ,  C12N9/0008 ,  C12N9/1288 ,  C12N9/16 ,  C12N9/88 ,  C12N15/63 ,  C12N15/74 ,  C12Y102/99006 ,  C12Y207/08007 ,  C12Y301/02014 ,  C12Y401/99005 ,  Y02P20/52

摘要： The present disclosure identifies methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently produce alkanes, and in particular the use of such organisms for the commercial production of alkanes and related molecules. Other materials, methods, and compositions are also described.

公开(公告)号：US09487813B2

公开(公告)日：2016-11-08

申请号：US13376172

申请日：2010-06-04

申请人： Jason David Oliver ,  Katharine Sarah Dobb ,  Sarah Jane Kaye ,  John Leslie Thain ,  Daniel Scott Tuckwell ,  Michael John Bromley

发明人： Jason David Oliver ,  Katharine Sarah Dobb ,  Sarah Jane Kaye ,  John Leslie Thain ,  Daniel Scott Tuckwell ,  Michael John Bromley

IPC分类号： C12Q1/18 ,  C12N9/12 ,  G01N21/64

CPC分类号： C12Q1/18 ,  C12N9/1288

摘要： A method of identifying an antifungal agent which targets a PPTB protein of a fungus comprising determining whether a candidate compound binds to or inhibits a PPTB protein, wherein binding or inhibition indicates that the candidate substance is an antifungal agent.

摘要翻译： 鉴定靶向真菌的PPTB蛋白的抗真菌剂的方法，包括确定候选化合物是否结合或抑制PPTB蛋白，其中结合或抑制表明候选物质是抗真菌剂。

公开(公告)号：US09340792B2

公开(公告)日：2016-05-17

申请号：US13805037

申请日：2011-06-16

申请人： Todd R. Klaenhammer ,  Erika Pfeiler ,  Mansour Mohamadzadeh

发明人： Todd R. Klaenhammer ,  Erika Pfeiler ,  Mansour Mohamadzadeh

IPC分类号： C12N15/74 ,  A61K35/744 ,  C12N1/20 ,  C12N9/12 ,  C12R1/23 ,  A61K35/00

CPC分类号： A61K35/747 ,  A61K35/744 ,  A61K2035/115 ,  C12N1/20 ,  C12N9/1288 ,  C12N15/746 ,  C12R1/23 ,  C12Y207/0802

摘要： Methods and compositions for treating or preventing inflammatory disorders are provided. The compositions of the invention comprise a recombinant bacterium genetically modified to decrease the display of lipoteichoic acid on the cell surface. Methods of the invention comprise administering to a subject a recombinant bacterium modified to decrease the display of lipoteichoic acid on the cell surface. Administration of the recombinant bacterium promotes a desired therapeutic response. The recombinant bacterium may be administered in a single dose or series of doses. Methods of the invention find use in treating or preventing a variety of inflammatory disorders including, for example, treating or preventing inflammatory bowel disease, colitis, or Crohn's disease.

摘要翻译： 提供了治疗或预防炎症性疾病的方法和组合物。 本发明的组合物包含经遗传修饰以减少细胞表面上脂磷壁酸显示的重组细菌。 本发明的方法包括向受试者施用经修饰以减少细胞表面上的脂磷壁酸显示的重组细菌。 重组细菌的施用促进期望的治疗反应。 重组细菌可以以单一剂量或一系列剂量施用。 本发明的方法可用于治疗或预防各种炎性疾病，包括例如治疗或预防炎症性肠病，结肠炎或克罗恩氏病。

公开(公告)号：US20160130568A1

公开(公告)日：2016-05-12

申请号：US14977349

申请日：2015-12-21

申请人： BATTELLE ENERGY ALLIANCE, LLC.

发明人： David N Thompson ,  William A Apel ,  Vicki S Thompson ,  David W Reed ,  Jeffrey A Lacey

IPC分类号： C12N9/10 ,  C12N9/12

CPC分类号： C12N9/1051 ,  C12N9/1048 ,  C12N9/1288 ,  C12P21/00 ,  C12P21/005 ,  C12Y204/00 ,  C12Y204/01083 ,  C12Y207/08

摘要： Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods for glycosylating and/or post-translationally modifying proteins using isolated and/or purified polypeptides and nucleic acid sequences from Alicyclobacillus acidocaldarius.

摘要翻译： 提供了分离和/或纯化的多肽和编码来自酸热环酸杆菌的多肽的核酸序列。 还提供了使用分离的和/或纯化的多肽和来自酸性脂环酸杆菌的核酸序列糖基化和/或翻译后修饰蛋白质的方法。