公开(公告)号：US20240240214A1

公开(公告)日：2024-07-18

申请号：US18561867

申请日：2022-05-17

Applicant: MINT BIOTECHNOLOGIES CO., LTD.

Inventor： Kechun ZHANG ,  Jingyu WANG ,  Jilong WANG

IPC: C12P13/06 ,  C12N9/10 ,  C12N9/88 ,  C12R1/07 ,  C12R1/15 ,  C12R1/19 ,  C12R1/465

CPC classification number: C12P13/06 ,  C12N9/1022 ,  C12N9/88 ,  C12R2001/07 ,  C12R2001/15 ,  C12R2001/19 ,  C12R2001/465 ,  C12Y202/01006 ,  C12Y403/01019

Abstract: A method for producing L-isoleucine at a higher yield by fermentation includes the step of using a genetic engineering method to obtain a genetically engineered strain. The genetically engineered strain has a threonine deaminase gene substantially releasing the inhibition of L-isoleucine and/or an acetylated hydroxy acid synthetase III gene substantially releasing the inhibition of L-isoleucine; and performing fermentation culture on the genetically engineered strain, adding diketobutyric acid or a raw material capable of being converted into diketobutyric acid in a culture process, and separating L-isoleucine from a culture after the end of culturing. Further provided is a genetically engineered strain for realizing high yield of L-isoleucine.

公开(公告)号：US20240191053A1

公开(公告)日：2024-06-13

申请号：US18555230

申请日：2022-04-12

Applicant: WASHINGTON UNIVERSITY

Inventor： Tae Seok Moon ,  Jinjin Diao ,  Yifeng Hu ,  Kirsten Davis

IPC: C08J11/10 ,  C12N9/10 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52

CPC classification number: C08J11/105 ,  C12N9/1022 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12Y202/01007 ,  C12Y401/01063 ,  C12Y503/03002

Abstract: Among the various aspects of the present disclosure is the provision of methods, systems, and Rhodococcus sp. strains for the upcycling of polyethylene terephthalate) (PET). An aspect of the present disclosure provides for a system for waste PET valorization comprising: a microorganism capable of growing on PET hydrolysis products, such as PET hydrolysate. PET products from chemical hydrolysis, or alkaline hydrolysis products of PET as a carbon source.

公开(公告)号：US20240124899A1

公开(公告)日：2024-04-18

申请号：US18458762

申请日：2023-08-30

Applicant: Board of Trustees of Michigan State University

Inventor： Björn Hamberger ,  Sean Johnson ,  Wajid Waheed Bhat

IPC: C12P5/00 ,  C12N9/10 ,  C12N15/82 ,  C12P17/02 ,  C12P17/06

CPC classification number: C12P5/002 ,  C12N9/1022 ,  C12N9/1085 ,  C12N15/8243 ,  C12P17/02 ,  C12P17/06 ,  C12Y202/01007 ,  C12Y205/01029

Abstract: Enzymes and methods are described herein for manufacturing terpenes, including terpenes.

公开(公告)号：US11859223B2

公开(公告)日：2024-01-02

申请号：US16091624

申请日：2017-04-07

Applicant: The Trustees of Princeton University

Inventor： Jose L. Avalos ,  Jared E. Toettcher ,  Evan M. Zhao

IPC: C12P7/56 ,  C12N9/04 ,  C12N9/88 ,  C12N9/10 ,  C12N13/00 ,  C12P7/16 ,  C12N15/81 ,  C12N15/63

CPC classification number: C12N9/88 ,  C12N9/0006 ,  C12N9/1022 ,  C12N13/00 ,  C12N15/63 ,  C12N15/635 ,  C12N15/81 ,  C12P7/16 ,  C12P7/56 ,  C12Y101/01027 ,  C12Y202/01006 ,  C12Y401/01001

Abstract: A system and method for controlling metabolic enzymes or pathways in cells to produce a chemical above the levels of a wild-type strain is disclosed. The system utilizes cells, including yeasts, bacteria, and molds, having at least two genes capable of being controlled bi-directionally with light, where one gene is turned from off to on when exposed to light and another gene is turned from on to off when exposed to light, the two genes reversing when the light is turned off. Cells may utilize any number of sequences that benefit chemical production, including sequences that: encode for constitutive transcription of light-activated transcription factor fusions; encode for a metabolic enzyme; encode for a repressor; induce expression of metabolic enzymes; and an endogenous or exogenous activator expressed by a constitutive promoter, inducible promoter, or gene circuit. These systems may be coupled to biosensors or protein cascade systems, enabling the monitoring or automation of the fermentation process to optimize production of a desired product. These systems may also allow for optimization and periodic operation of a bioreactor using light pulses.

公开(公告)号：US20230313241A1

公开(公告)日：2023-10-05

申请号：US18059330

申请日：2022-11-28

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Ja Kyong KO ,  Youngyun JO ,  Gyeongtaek GONG ,  Jung Ho AHN ,  Sun Mi LEE ,  Youngsoon UM

IPC: C12P7/625 ,  C12N15/74 ,  C12N9/10

CPC classification number: C12P7/625 ,  C12N15/74 ,  C12N9/1022 ,  C12Y202/01006 ,  C12N9/1029 ,  C12Y203/01009 ,  C12Y203/01182

Abstract: The present disclosure provides a recombinant microorganism for producing PHBV, a method for preparing the same, and a method for producing PHBV using the microorganism. The present disclosure may provide a recombinant microorganism capable of producing PHBV, which is a biodegradable plastic material with superior physical properties, directly from an inexpensive single carbon source with high efficiency without supplementation of organic acid. The present disclosure can enhance the utilization of PHA, which is expensive and has limited physical properties, and can also provide a technology more effective for industrialization using an inexpensive single carbon source. The PHBV produced according to an exemplary embodiment of the present disclosure can be used not only for general-purpose inexpensive products such as ecofriendly packing materials but also as a high-value-added medical biopolymer.

公开(公告)号：US11739338B2

公开(公告)日：2023-08-29

申请号：US16648625

申请日：2018-09-18

Applicant: Lallemand Hungary Liquidity Management LLC

Inventor： Brooks Henningsen ,  Sean Covalla ,  Rintze Zelle ,  Allan Froehlich

IPC: C12N15/81 ,  C07K14/395 ,  C12N9/04 ,  C12N9/10 ,  C12N9/12 ,  C12N9/90 ,  C12N9/92 ,  C12N9/00 ,  C12P7/06

CPC classification number: C12N15/81 ,  C07K14/395 ,  C12N9/0006 ,  C12N9/1022 ,  C12N9/12 ,  C12N9/1205 ,  C12N9/90 ,  C12N9/92 ,  C12N9/93 ,  C12P7/06 ,  C12Y101/01001 ,  C12Y202/01002 ,  C12Y207/01017 ,  C12Y207/11001 ,  C12Y501/03001 ,  C12Y503/01006 ,  C12Y602/01001

Abstract: Acetate is a potent microbial inhibitor which can affect the performance of yeast in ethanolic fermentation. The present disclosure provides a recombinant microbial host cell having (i) a first genetic modification for increasing the activity of one or more proteins that function in a first metabolic pathway to convert acetate into an alcohol in the microbial host cell; (ii) a second genetic modification for increasing the activity of one or more proteins that function in a second metabolic pathway to import glycerol in the recombinant microbial host cell (iii) a third genetic modification for increasing the activity of one or more proteins that function in a third metabolic pathway to convert a C5 carbohydrate into ethanol in the microbial host cell. The recombinant microbial host cell comprises and natively expresses native proteins that function in a fourth native metabolic pathway to produce glycerol in the microbial host cell.

公开(公告)号：US20230212588A1

公开(公告)日：2023-07-06

申请号：US17806138

申请日：2022-06-09

Applicant: Bio Capital Holdings, LLC

Inventor： Raul CUERO RENGIFO

IPC: C12N15/52 ,  C12N9/10 ,  C12N9/12 ,  C12N9/88 ,  C12N9/90 ,  C12N15/82

CPC classification number: C12N15/52 ,  C12N9/88 ,  C12N9/90 ,  C12N9/1022 ,  C12N9/1051 ,  C12N9/1085 ,  C12N9/1205 ,  C12N15/8243 ,  C12Y202/01007 ,  C12Y204/01255 ,  C12Y205/01029 ,  C12Y207/01001 ,  C12Y207/01036 ,  C12Y401/01033 ,  C12Y503/03002

Abstract: Described herein are devices and methods for increasing the production of steviol glycosides, which have industrial and economic value. The steviol glycosides produced by the devices and methods disclosed herein do not require the ultra purification that is common in conventional or commercial methods and do not have a bitter aftertaste, making them better suited as flavor-enhancing additives to food, pharmaceutical, and nutritional supplement products.

公开(公告)号：US10059967B2

公开(公告)日：2018-08-28

申请号：US14373160

申请日：2013-01-18

Applicant: Genomatica, Inc.

Inventor： Robin E. Osterhout ,  Anthony P. Burgard ,  Mark J. Burk

IPC: C12P7/44 ,  C12N1/20 ,  C12N15/00 ,  C12N9/02 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C12N9/12 ,  C12N9/88 ,  C08G63/183 ,  C12P7/24 ,  C12P7/40 ,  C12P9/00 ,  C07F9/09 ,  C07C51/43 ,  C12P7/62

CPC classification number: C12P7/44 ,  C07C51/43 ,  C07F9/091 ,  C08G63/183 ,  C12N9/0004 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1007 ,  C12N9/1022 ,  C12N9/1085 ,  C12N9/1205 ,  C12N9/88 ,  C12P7/24 ,  C12P7/40 ,  C12P7/62 ,  C12P9/00 ,  C07C63/26 ,  C07C63/04

Abstract: The invention provides non-naturally occurring microbial organisms having a (2-hydroxy-3-methyl-4-oxobutoxy) phosphonate (2H3M40P) pathway, p-toluate pathway, and/or terephthalate pathway. The invention additionally provides methods of using such organisms to produce 2H3M40P, p-toluate or terephthalate. Also provided herein are processes for isolating bio-based aromatic carboxylic acid, in particular, p-toluic acid or terephthalic acid, from a culture medium, wherein the processes involve contacting the culture medium with sufficient carbon dioxide (CO2) to lower the pH of the culture medium to produce a precipitate comprised of the aromatic carboxylic acid.

公开(公告)号：US10000760B2

公开(公告)日：2018-06-19

申请号：US14976636

申请日：2015-12-21

Applicant: AJINOMOTO CO., INC.

Inventor： Misato Morita ,  Hiroaki Nishiuchi

IPC: C12N1/14 ,  C12N15/00 ,  C12N9/88 ,  C12N9/10 ,  C12N9/16 ,  C12P13/00 ,  C07H21/04 ,  C12N15/81 ,  C12N1/16 ,  C12P13/04 ,  C12P13/14 ,  A23L27/21 ,  A23L27/23 ,  A23L33/14 ,  A23L33/145

CPC classification number: C12N15/815 ,  A23L27/21 ,  A23L27/23 ,  A23L33/14 ,  A23L33/145 ,  A23V2002/00 ,  C12N1/16 ,  C12N9/1022 ,  C12N9/1025 ,  C12N9/88 ,  C12N15/81 ,  C12P13/04 ,  C12P13/14 ,  C12Y202/01006

Abstract: Yeast and a yeast extract with a high content of Abu, γ-Glu-Abu, and/or γ-Glu-Abu-Gly are provided. By modifying yeast so that intracellular acetolactate synthase activity is reduced, yeast with a high content of Abu, γ-Glu-Abu, and/or γ-Glu-Abu-Gly is obtained. An yeast extract is prepared by using the yeast obtained in such a manner as a raw material.

公开(公告)号：US09862975B2

公开(公告)日：2018-01-09

申请号：US14858571

申请日：2015-09-18

Applicant: CARGILL INCORPORATED

Inventor： Holly J. Jessen ,  Jian Yi ,  Joshua Lundorff ,  Hans Liao ,  Ana Negrete-Raymond ,  Pirkko Suominen ,  Aristos Aristidou

IPC: C12N1/00 ,  C12N1/19 ,  C12N1/22 ,  C12P7/06 ,  C12N9/12 ,  C12N9/90 ,  C12P7/10 ,  C12N15/81 ,  C07K14/39 ,  C12N9/04 ,  C12N9/10 ,  C12N9/92 ,  C12P7/54

CPC classification number: C12P7/10 ,  C07K14/39 ,  C12N9/0006 ,  C12N9/1022 ,  C12N9/1205 ,  C12N9/90 ,  C12N9/92 ,  C12N15/815 ,  C12P7/06 ,  C12P7/54 ,  C12Y101/01 ,  C12Y101/01009 ,  C12Y101/0101 ,  C12Y202/01001 ,  C12Y202/01002 ,  C12Y207/01016 ,  C12Y207/01017 ,  C12Y501/03001 ,  C12Y501/03004 ,  C12Y503/01003 ,  C12Y503/01005 ,  C12Y503/01006 ,  Y02E50/16 ,  Y02E50/17

Abstract: The present application discloses the identification of the novel K. marxianus xylose transporter genes KHT105 and RAG4, as well as the identification of a novel set of I. orientalis pentose phosphate pathway genes The present application further discloses a series of genetically modified yeast cells comprising various combinations of arabinose fermentation pathways, xylose fermentation pathways, pentose phosphate pathways, and/or xylose transporter genes, and methods of culturing these cells to produce ethanol in fermentation media containing xylose.