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公开(公告)号：US20240318212A1
公开(公告)日：2024-09-26
申请号：US18599745
申请日：2024-03-08
发明人： Nan-Wei SU , Hsin-Ya TSAI , Chen HSU
CPC分类号： C12P17/181 , C12N9/1229 , C12N9/93 , C12P17/06 , C12Y207/04001 , C12Y602/01005
摘要： The present invention provides a coupled enzyme system, comprises: a first enzyme, comprising a polyphenol phosphorylation synthetase; a second enzyme, which is ATP regeneration enzyme; and a substrate, being phosphorylated by the first enzyme. The coupled enzyme system of the present invention integrates polyphenol phosphorylation synthetase with ATP regeneration enzyme so that the polyphenol phosphorylation synthetase is used to phosphorylate polyphenol and the ATP regeneration enzyme regenerate ATP from AMP. Therefore, the present invention not only improves the water-solubility and bioavailability of the phenolic phytochemicals but also significantly reduces ATP consumption, presenting the potential of enzymatic systems in the production of polyphenol monophosphates.
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公开(公告)号：US20240318211A1
公开(公告)日：2024-09-26
申请号：US18587332
申请日：2024-02-26
CPC分类号： C12P17/06 , C12N9/1085 , C12N9/1205 , C12N9/1229 , C12N9/90 , C12P7/02 , C12Y205/01001 , C12Y205/01039 , C12Y207/0105 , C12Y207/04026 , C12Y503/03002
摘要： Ways of making and using a recombinant organism configured to produce a cannabinoid are provided. The recombinant organism can include a eukaryotic microorganism expressing a recombinant construct including a geranyl diphosphate synthase (GPPS2), an isopentenyl diphosphate isomerase (IDI), an isopentenyl phosphate kinase (IPK), and a 5-(hydroxyethyl)-methyl thiazole kinase (ThiM). A cannabinoid can be produced by a process that includes growing the recombinant organism configured to produce the cannabinoid in a growth medium and separating the cannabinoid from the recombinant organism and the growth medium. A biosynthetic system for producing a cannabinoid is provided that includes a bioreactor, the recombinant organism configured to produce the cannabinoid, and a growth medium for the recombinant organism.
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公开(公告)号：US20240294957A1
公开(公告)日：2024-09-05
申请号：US18350985
申请日：2023-07-12
发明人： Tomás Pluskal , Jing-Ke Weng
CPC分类号： C12P17/06 , C12P7/26 , C12Y301/27005
摘要： Disclosed are methods, compositions, proteins, nucleic acids, cells, vectors, compounds, reagents, and systems for the preparation of kavalactones, flavokavains, and kavalactone and flavokavain biosynthetic intermediates using enzymes expressed in heterologous host cells, such as microorganisms or plants, or using in vitro enzymatic reactions. This invention also provides for the expression of the enzymes by recombinant cell lines and vectors. Furthermore, the enzymes can be components of constructs such as fusion proteins. The kavalactones produced can be utilized to treat anxiety disorder, insomnia, and other psychological and neurological disorders. The flavokavains produced can be utilized to treat various cancers including colon, bladder, and breast cancers.
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公开(公告)号：US20240240160A1
公开(公告)日：2024-07-18
申请号：US18564967
申请日：2022-05-27
申请人： GENOMATICA, INC.
CPC分类号： C12N9/1029 , C12N15/52 , C12N15/70 , C12P7/22 , C12P7/42 , C12P17/06 , C12P19/32 , C12Y203/01206
摘要： The present disclosure provides a poly nucleotide comprising: (a) a nucleic acid sequence encoding an olivetol synthase (OLS): and (b) a heterologous regulatory element operably linked to the nucleic acid sequence. The present disclosure further relates to an engineered cell comprising an olivetol synthase (OLS). Also provided are a cell extract or cell culture medium or a composition comprising 3.5.7- trioxododecanoyl-CoA, olivetol, olivetolic acid, a cannabinoid, and/or an isomer, analog, or derivative thereof, and a method of making 3.5.7-trioxododecanoyl-CoA, olivetol, olivetolic acid, a cannabinoid, and/or an isomer, analog, or derivative thereof. Also provided is a cannabinoid produced by the engineered cell, isolated from the cell extract or cell culture medium, and/or made by the method described herein. The present disclosure also provides a non-natural olivetol synthase (OLS).
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公开(公告)号：US11965197B2
公开(公告)日：2024-04-23
申请号：US17197468
申请日：2021-03-10
发明人： Mattheos Koffas , John Andrew Jones
IPC分类号： C12N9/90 , C12N9/00 , C12N9/02 , C12N9/04 , C12N9/10 , C12N9/88 , C12N15/52 , C12P7/42 , C12P7/52 , C12P17/06 , C12P19/58
CPC分类号： C12P19/58 , C12N9/0006 , C12N9/0071 , C12N9/0093 , C12N9/1037 , C12N9/88 , C12N9/90 , C12N9/93 , C12N15/52 , C12P7/42 , C12P7/52 , C12P17/06 , C12Y101/01219 , C12Y114/11009 , C12Y117/01003 , C12Y203/01074 , C12Y403/01023 , C12Y505/01006 , C12Y602/01012 , C12Y602/01014
摘要： Disclosed herein are novel microbial polycultures of two or more cell strains, capable of producing flavanones, flavonoids, and anthocyanidin-3-O-glucosides, and methods of use thereof. Also disclosed is a microbial cell capable of producing phenylpropanoic acids, and methods of use thereof.
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公开(公告)号：US20240124905A1
公开(公告)日：2024-04-18
申请号：US18274445
申请日：2022-01-26
CPC分类号： C12P7/42 , C12N9/1085 , C12N15/81 , C12P17/06 , C12Y205/01029 , C12N2800/102
摘要： Provided is a nucleic acid comprising a recombinant bacterial or archaeal geranyl pyrophosphate synthase (GPPS) gene, codon optimized for production in yeast. Also provided is a yeast cell comprising an expression cassette comprising the above nucleic acid. Additionally provided is a method of producing a terpene or cannabinoid in a yeast, the method comprising incubating the above yeast cell in a manner sufficient to produce the terpene or cannabinoid.
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公开(公告)号：US20240052387A1
公开(公告)日：2024-02-15
申请号：US17766552
申请日：2020-10-08
申请人： JOHANN WOKFGANG GOETHE-UNIVERSITAT FRANKFURT AM MAIN , ROSALIND FRANKLIN UNIVERSITY OF MEDICINE AND SCIENCE
发明人： SIMON HARTH , MISLAV OREB , JUN-YONG CHOE
CPC分类号： C12P17/06 , C12P7/58 , C12N9/0006 , C12Y101/01067 , C12Y101/01 , C12N15/81 , C12N2800/102
摘要： The present invention relates to polypeptides which are galacturonate (GalA) reductase variants comprising at least one amino acid substitution at a position corresponding to K261 and/or R267. The present invention further relates to nucleic acid molecules encoding the polypeptides and to host cells containing said nucleic acid molecules. The present invention further relates to a method for the production of L-galactonate (GalOA) and/or other bio-based compounds, comprising the expression of said nucleic acid molecules, preferably in said host cells. The present invention also relates to the use of the polypeptides, nucleic acids molecule or host cells for the production of L-galactonate (GalOA) and/or other bio-based compounds, and/or for the recombinant fermentation of biomaterial containing D-galacturonate (GalA).
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公开(公告)号：US20230323414A1
公开(公告)日：2023-10-12
申请号：US18023946
申请日：2021-08-26
申请人： DAICEL CORPORATION
摘要： An object of the present disclosure is at least to provide an enzyme that dehydroxylates a hydroxyl group at a 9-position of urolithins having a hydroxyl group at the 9-position, and the object is solved by an enzyme having properties (1) and (2) below: (1) The enzyme dehydroxylates a hydroxyl group at a 9-position of urolithins; and (2) the enzyme is activated by reduced nicotinamide adenine dinucleotide (NADH) and/or reduced nicotinamide adenine dinucleotide phosphate (NADPH).
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9.发明公开公开(公告)号：US20230313246A1
公开(公告)日：2023-10-05
申请号：US18132514
申请日：2023-04-10
发明人： Yanju Li , Zhengli Yang , Yongni Wu , Aiping Zhang
CPC分类号： C12P17/06 , C12N1/145 , C12R2001/77
摘要： The present invention discloses application of a strain CH18 in preparation of anthocyanins and other flavonoid compounds. The preservation number of the strain CH18 is CGMCC NO.14784. Flavonoid compounds have the functions of resisting oxidation, tumors and fatigue, delaying senescence, preventing cardiovascular and cerebrovascular diseases, reducing blood pressure and blood lipid, regulating neuroendocrine, resisting osteoporosis, removing freckles, resisting wrinkles, and improving eyesight. The present invention uses microorganisms to produce anthocyanins and other flavonoids, which has the advantages of low price, easy control, short production cycle and year-round production, and can meet the needs of industrial production. Therefore, the present invention opens up a new biological pathway for producing a variety of anthocyanins and other flavonoid compounds with high efficiency and low cost.
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公开(公告)号：US11739354B2
公开(公告)日：2023-08-29
申请号：US17190872
申请日：2021-03-03
发明人： Tomás Pluskal , Jing-Ke Weng
CPC分类号： C12P17/06 , C12P7/26 , C12Y301/27005
摘要： Disclosed are methods, compositions, proteins, nucleic acids, cells, vectors, compounds, reagents, and systems for the preparation of kavalactones, flavokavains, and kavalactone and flavokavain biosynthetic intermediates using enzymes expressed in heterologous host cells, such as microorganisms or plants, or using in vitro enzymatic reactions. This invention also provides for the expression of the enzymes by recombinant cell lines and vectors. Furthermore, the enzymes can be components of constructs such as fusion proteins. The kavalactones produced can be utilized to treat anxiety disorder, insomnia, and other psychological and neurological disorders. The flavokavains produced can be utilized to treat various cancers including colon, bladder, and breast cancers.
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