公开(公告)号：US20240218403A1

公开(公告)日：2024-07-04

申请号：US18285138

申请日：2022-04-01

申请人： Ginkgo Bioworks, Inc.

发明人： Guillaume Beaudoin ,  Elena Brevnova ,  Alkiviadis Orfefs Chatzivasileiou ,  Alexandra Exner ,  Annapuma Kamineni ,  Matthew McMahon ,  Joshua Trueheart

IPC分类号： C12P7/42 ,  C12N1/16 ,  C12N1/20 ,  C12N9/04 ,  C12N9/10 ,  C12N9/12 ,  C12N9/88 ,  C12N9/90 ,  C12P17/02 ,  C12P33/00 ,  C12R1/19 ,  C12R1/865

CPC分类号： C12P7/42 ,  C12N1/165 ,  C12N1/205 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/1205 ,  C12N9/1229 ,  C12N9/88 ,  C12N9/90 ,  C12P17/02 ,  C12P33/00 ,  C12R2001/19 ,  C12R2001/865 ,  C12Y101/01034 ,  C12Y101/01088 ,  C12Y203/01009 ,  C12Y203/0301 ,  C12Y207/01036 ,  C12Y207/04002 ,  C12Y207/04026 ,  C12Y401/01033 ,  C12Y503/03002 ,  C12Y504/99007

摘要： Described in this application are proteins and host cells involved in methods of producing isoprenoid precursors and/or isoprenoids.

公开(公告)号：US20120202234A1

公开(公告)日：2012-08-09

申请号：US13384534

申请日：2010-07-19

申请人： Joseph P. Noel ,  Nikki M. Dellas

发明人： Joseph P. Noel ,  Nikki M. Dellas

IPC分类号： C12P9/00 ,  C12Q1/48 ,  C12N9/12

CPC分类号： C12N9/1229 ,  C12N9/1217 ,  C12P9/00 ,  C12Y207/04026

摘要： Provided herein are methods and compositions relating to the synthesis of isoprenoid diphosphates using a mutated isopentenyl phosphate kinase.

摘要翻译： 本文提供了使用突变的异戊烯磷酸激酶合成类异戊二烯二磷酸的方法和组合物。

公开(公告)号：US20160002672A1

公开(公告)日：2016-01-07

申请号：US14654424

申请日：2013-12-20

申请人： DANISCO US INC. ,  THE GOODYEAR TIRE & RUBBER COMPANY

发明人： Zachary Q. BECK ,  Jörg MAMPEL ,  Guido MEURER ,  Michael C. MILLER ,  Karl J. SANFORD ,  Dmitrii V. VAVILINE ,  Walter WEYLER ,  Gregory M. WHITED

IPC分类号： C12P5/00 ,  C07F9/09 ,  C12N9/12 ,  C12P9/00 ,  C12N9/88 ,  C07C11/18

CPC分类号： C12P5/007 ,  C07C11/18 ,  C07F9/09 ,  C12N9/1229 ,  C12N9/88 ,  C12P9/00 ,  C12Y207/04026 ,  C12Y401/01 ,  Y02E50/343

摘要： The invention provides for compositions and methods for the production of isoprene, isoprenoid precursor, and/or isoprenoids in cells via the expression (e.g., heterologous expression) of phosphomevalonate decarboxylases and/or isopentenyl kinases.

摘要翻译： 本发明提供了通过磷酸戊糖醛酸脱羧酶和/或异戊烯基激酶的表达（例如异源表达）在细胞中产生异戊二烯，类异戊二烯前体和/或类异戊二烯的组合物和方法。

公开(公告)号：US12084704B2

公开(公告)日：2024-09-10

申请号：US16612065

申请日：2018-04-19

申请人： Global Bioenergies

发明人： Romain Chayot ,  Mathieu Allard ,  Maria Anissimova

IPC分类号： C12P5/02

CPC分类号： C12P5/026 ,  C12Y205/01 ,  C12Y207/0105 ,  C12Y207/04026 ,  C12Y207/06002 ,  C12Y207/06003 ,  C12Y401/01 ,  C12Y503/03002

摘要： Described are methods for the production of isobutene comprising the enzymatic conversion of 3-methylcrotonic acid into isobutene wherein the enzymatic conversion of 3-methylcrotonic acid into isobutene is achieved by making use of an FMN-dependent decarboxylase associated with an FMN prenyl transferase, wherein said FMN prenyl transferase catalyzes the prenylation of a flavin cofactor (FMN or FAD) utilizing dimethylallyl phosphate (DMAP) into a flavin-derived cofactor, wherein said method further comprises providing said DMAP enzymatically by: (i) the enzymatic conversion of dimethylallyl pyrophosphate (DMAPP) into said DMAP; or (ii) a single enzymatic step in which prenol is directly enzymatically converted into said DMAP; or (iii) two enzymatic steps comprising: first enzymatically converting DMAPP into prenol; and then enzymatically converting the thus obtained prenol into said DMAP; or (iv) the enzymatic conversion of isopentenyl monophosphate (IMP) into said DMAP, or by a combination of any one of (i) to (iv). Moreover, described are methods for the production of isobutene comprising the enzymatic conversion of 3-methylcrotonic acid into isobutene wherein the enzymatic conversion of 3-methylcrotonic acid into isobutene is achieved by making use of an FMN-dependent decarboxylase associated with an FMN prenyl transferase, wherein said FMN prenyl transferase catalyzes the prenylation of a flavin cofactor (FMN or FAD) utilizing dimethylallyl pyrophosphate (DMAPP), wherein said method further comprises providing said DMAPP enzymatically by: (v) the enzymatic conversion of isopentenyl pyrophosphate (IPP) into said DMAPP; or (vi) the enzymatic conversion of dimethylallyl phosphate (DMAP) into said DMAPP; or (vii) the enzymatic conversion of prenol into said DMAPP; (viii) or by a combination of any one of (v) to (vii). Moreover, described are methods for providing said flavin cofactor enzymatically by the enzymatic conversion of riboflavin into flavin mononucleotide (FMN).

公开(公告)号：US09051555B2

公开(公告)日：2015-06-09

申请号：US13384534

申请日：2010-07-19

申请人： Joseph P. Noel ,  Nikki M. Dellas

发明人： Joseph P. Noel ,  Nikki M. Dellas

IPC分类号： C12N9/12 ,  C12P9/00

CPC分类号： C12N9/1229 ,  C12N9/1217 ,  C12P9/00 ,  C12Y207/04026

摘要： Provided herein are methods and compositions relating to the synthesis of isoprenoid diphosphates using a mutated isopentenyl phosphate kinase.

摘要翻译： 本文提供了使用突变的异戊烯磷酸激酶合成类异戊二烯二磷酸的方法和组合物。

公开(公告)号：US20240318211A1

公开(公告)日：2024-09-26

申请号：US18587332

申请日：2024-02-26

申请人： 1000128725 Ontario Inc.

发明人： Hamed Hasheminejad ,  Mahdi Shahsavand

IPC分类号： C12P17/06 ,  C12N9/10 ,  C12N9/12 ,  C12N9/90 ,  C12P7/02

CPC分类号： C12P17/06 ,  C12N9/1085 ,  C12N9/1205 ,  C12N9/1229 ,  C12N9/90 ,  C12P7/02 ,  C12Y205/01001 ,  C12Y205/01039 ,  C12Y207/0105 ,  C12Y207/04026 ,  C12Y503/03002

摘要： Ways of making and using a recombinant organism configured to produce a cannabinoid are provided. The recombinant organism can include a eukaryotic microorganism expressing a recombinant construct including a geranyl diphosphate synthase (GPPS2), an isopentenyl diphosphate isomerase (IDI), an isopentenyl phosphate kinase (IPK), and a 5-(hydroxyethyl)-methyl thiazole kinase (ThiM). A cannabinoid can be produced by a process that includes growing the recombinant organism configured to produce the cannabinoid in a growth medium and separating the cannabinoid from the recombinant organism and the growth medium. A biosynthetic system for producing a cannabinoid is provided that includes a bioreactor, the recombinant organism configured to produce the cannabinoid, and a growth medium for the recombinant organism.

公开(公告)号：US20150307858A1

公开(公告)日：2015-10-29

申请号：US14701389

申请日：2015-04-30

申请人： The Salk Institute for Biological Studies

发明人： Joseph P. Noel ,  Nikki M. Dellas

IPC分类号： C12N9/12

CPC分类号： C12N9/1229 ,  C12N9/1217 ,  C12P9/00 ,  C12Y207/04026

摘要： Provided herein are methods and compositions relating to the synthesis of isoprenoid diphosphates using a mutated isopentenyl phosphate kinase.

摘要翻译： 本文提供了使用突变的异戊烯磷酸激酶合成类异戊二烯二磷酸的方法和组合物。