公开(公告)号：US20240318211A1

公开(公告)日：2024-09-26

申请号：US18587332

申请日：2024-02-26

申请人： 1000128725 Ontario Inc.

发明人： Hamed Hasheminejad ,  Mahdi Shahsavand

IPC分类号： C12P17/06 ,  C12N9/10 ,  C12N9/12 ,  C12N9/90 ,  C12P7/02

CPC分类号： C12P17/06 ,  C12N9/1085 ,  C12N9/1205 ,  C12N9/1229 ,  C12N9/90 ,  C12P7/02 ,  C12Y205/01001 ,  C12Y205/01039 ,  C12Y207/0105 ,  C12Y207/04026 ,  C12Y503/03002

摘要： Ways of making and using a recombinant organism configured to produce a cannabinoid are provided. The recombinant organism can include a eukaryotic microorganism expressing a recombinant construct including a geranyl diphosphate synthase (GPPS2), an isopentenyl diphosphate isomerase (IDI), an isopentenyl phosphate kinase (IPK), and a 5-(hydroxyethyl)-methyl thiazole kinase (ThiM). A cannabinoid can be produced by a process that includes growing the recombinant organism configured to produce the cannabinoid in a growth medium and separating the cannabinoid from the recombinant organism and the growth medium. A biosynthetic system for producing a cannabinoid is provided that includes a bioreactor, the recombinant organism configured to produce the cannabinoid, and a growth medium for the recombinant organism.