公开(公告)号：US20230348866A1

公开(公告)日：2023-11-02

申请号：US17786913

申请日：2020-12-24

申请人： The Regents of the University of California

发明人： James U. Bowie ,  Tyler P. Korman ,  Meaghan Valliere

IPC分类号： C12N9/10 ,  C12P7/6427 ,  C12N15/52

CPC分类号： C12N9/1085 ,  C12Y205/01039 ,  C12P7/6427 ,  C12N15/52

摘要： Provided is an enzyme useful for prenylation and recombinant pathways for the production of cannabinoids, cannabinoid precursors and other prenylated chemicals in a cell free system as well and recombinant microorganisms that catalyze the reactions.

公开(公告)号：US20240093253A1

公开(公告)日：2024-03-21

申请号：US17767957

申请日：2020-10-12

申请人： National University of Singapore

发明人： Kevin Jie Han Lim ,  Maybelle Darlene Kho Go ,  Wen Shan Yew

IPC分类号： C12P17/06 ,  C12N9/00 ,  C12N9/10 ,  C12N9/88 ,  C12N15/52 ,  C12N15/81

CPC分类号： C12P17/06 ,  C12N9/1029 ,  C12N9/1085 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12N15/81 ,  C12Y203/01086 ,  C12Y203/01206 ,  C12Y205/01039 ,  C12Y404/01026 ,  C12Y602/01012 ,  C12Y602/01025 ,  C12Y602/0103 ,  C12N2800/102

摘要： A recombinant cell of Saccharomyces cerevisiae that includes in its genome nucleic acids encoding cannabinoid biosynthetic pathway genes. A cannabinoid is produced by the recombinant cell in the presence of a cannabinoid precursor substrate and at least one of the cannabinoid biosynthetic pathway genes is from an organism other than Cannabis sativa, wherein the at least one of the cannabinoid biosynthetic pathway genes encodes a prenyltransferase. In an embodiment, the prenyltransferase is NphB from Streptomyces sp. having the amino acid sequence of any one of SEQ ID NOs: 8-11. Also disclosed is a method for producing a cannabinoid with the recombinant cell and the cannabinoid precursor substrate.

公开(公告)号：US20230193221A1

公开(公告)日：2023-06-22

申请号：US18049221

申请日：2022-10-24

申请人： The Regents of the University of California

发明人： James U. Bowie ,  Meaghan Valliere ,  Tyler P. Korman ,  Nicholas Woodall

IPC分类号： C12N9/10 ,  C12P7/22 ,  C12P7/42 ,  C12P9/00 ,  C12P17/06

CPC分类号： C12N9/1085 ,  C12P7/22 ,  C12P7/42 ,  C12P9/00 ,  C12P17/06 ,  C12Y205/01039

摘要： Provided is an enzyme useful for prenylation and recombinant pathways for the production of cannabinoids, cannabinoid precursors and other prenylated chemicals in a cell free system as well and recombinant microorganisms that catalyze the reactions.

公开(公告)号：US20240318211A1

公开(公告)日：2024-09-26

申请号：US18587332

申请日：2024-02-26

申请人： 1000128725 Ontario Inc.

发明人： Hamed Hasheminejad ,  Mahdi Shahsavand

IPC分类号： C12P17/06 ,  C12N9/10 ,  C12N9/12 ,  C12N9/90 ,  C12P7/02

CPC分类号： C12P17/06 ,  C12N9/1085 ,  C12N9/1205 ,  C12N9/1229 ,  C12N9/90 ,  C12P7/02 ,  C12Y205/01001 ,  C12Y205/01039 ,  C12Y207/0105 ,  C12Y207/04026 ,  C12Y503/03002

摘要： Ways of making and using a recombinant organism configured to produce a cannabinoid are provided. The recombinant organism can include a eukaryotic microorganism expressing a recombinant construct including a geranyl diphosphate synthase (GPPS2), an isopentenyl diphosphate isomerase (IDI), an isopentenyl phosphate kinase (IPK), and a 5-(hydroxyethyl)-methyl thiazole kinase (ThiM). A cannabinoid can be produced by a process that includes growing the recombinant organism configured to produce the cannabinoid in a growth medium and separating the cannabinoid from the recombinant organism and the growth medium. A biosynthetic system for producing a cannabinoid is provided that includes a bioreactor, the recombinant organism configured to produce the cannabinoid, and a growth medium for the recombinant organism.

公开(公告)号：US20240084337A1

公开(公告)日：2024-03-14

申请号：US17767722

申请日：2020-10-12

申请人： National University of Singapore

发明人： Maybelle Darlene Kho Go ,  Kevin Jie Han Lim ,  Yan Ping Lim ,  Wen Shan Yew

IPC分类号： C12P7/42 ,  C12N9/10 ,  C12N15/81

CPC分类号： C12P7/42 ,  C12N9/1085 ,  C12N15/81 ,  C12Y205/01039

摘要： A method for producing a cannabinoid precursor by contacting a substrate and geranyl pyrophosphate or farnesyl pyrophosphate with an NphB orthologue. The NphB orthologue is from an organism other than Cannabis sativa, and the substrate can be 2,4-dihydroxy-6-pentylbenzoic acid or 2,4-dihydroxy-6-propylbenzoic acid. Also disclosed is a recombinant cell of Yarrowia lipolytica, carrying in its genome a nucleic acid encoding an NphB orthologue from an organism other than Cannabis sativa such that the NphB orthologue is expressed in the recombinant cell.

公开(公告)号：US20170275659A1

公开(公告)日：2017-09-28

申请号：US15466352

申请日：2017-03-22

申请人： REGENTS OF THE UNIVERSITY OF MINNESOTA

发明人： Michael Joseph Smanski ,  Szu-Yi Hsu

IPC分类号： C12P15/00 ,  C12N9/88 ,  C12N9/10 ,  C12N15/52

CPC分类号： C12P15/00 ,  C12N9/1085 ,  C12N9/88 ,  C12N15/52 ,  C12Y205/01039

摘要： This disclosure describes recombinant cells and methods for microbial biosynthesis of ent-atiserenoic acid. Thus, in one aspect, this disclosure describes a recombinant cell genetically modified to exhibit increased biosynthesis of ent-atiserenoic acid compared to a comparable control cell. In some cases, the recombinant cell can include a host cell modified to include at least one heterologous polynucleotide encoding at least one enzyme in a biosynthetic pathway that produces ent-atiserenoic acid. In some cases, the recombinant cell can include a host cell and at least one heterologous enzyme in a biosynthetic pathway that produces ent-atiserenoic acid.