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    GENETICALLY ENGINEERED MICROBES AND METHODS FOR PRODUCING CITRAMALATE
    2.
    发明申请
    GENETICALLY ENGINEERED MICROBES AND METHODS FOR PRODUCING CITRAMALATE 审中-公开

    公开(公告)号：US20190093135A1

    公开(公告)日：2019-03-28

    申请号：US16086883

    申请日：2017-03-21

    申请人： UNIVERSITY OF GEORGIA RESEARCH FOUNDATION, INC.

    发明人： Xianghao Wu ,  Mark Eiteman

    IPC分类号： C12P7/46 ,  C12N9/10

    CPC分类号： C12P7/46 ,  C12N9/10 ,  C12N9/1025 ,  C12N9/1029 ,  C12N15/70 ,  C12Y203/01182 ,  C12Y203/03001

    摘要： Provided herein is a genetically engineered microbe which accumulates citramalate. In one embodiment, the microbe includes an exogenous polynucleotide encoding a citramalate synthase which catalyzes the condensation of acetyl CoA and pyruvic acid. Optionally, the microbe also includes a second exogenous polynucleotide encoding a citrate synthase which catalyzes the condensation of acetyl CoA and oxaloacetate, and the citrate synthase activity in the microbe is reduced compared to a control microbe. In one embodiment, the citrate synthase includes at least one amino acid substitution in the acetyl-CoA binding pocket, the mobile loop, the NADH binding site, and the oxaloacetate binding site, or a combination thereof. Also provided herein are methods for using the genetically engineered microbe, including a method for producing citramalate. The method can further include isolating the citramalate.

    Branched-Chain Fatty Acids And Biological Production Thereof
    3.
    发明申请
    Branched-Chain Fatty Acids And Biological Production Thereof 审中-公开
    分支链脂肪酸及其生物生产

    公开(公告)号：US20110151526A1

    公开(公告)日：2011-06-23

    申请号：US12972822

    申请日：2010-12-20

    申请人： Charles Winston Saunders ,  Jun Xu ,  Phillip Richard Green ,  David Blair Cody ,  Zubin Saresh Khambatta

    发明人： Charles Winston Saunders ,  Jun Xu ,  Phillip Richard Green ,  David Blair Cody ,  Zubin Saresh Khambatta

    IPC分类号： C12P7/64 ,  C12N1/21

    CPC分类号： C12N9/1029 ,  C12N9/0008 ,  C12N9/88 ,  C12N15/70 ,  C12P7/6409 ,  C12Y102/04004 ,  C12Y203/0118 ,  C12Y203/01182 ,  C12Y403/01019

    摘要： A method for producing anteiso fatty acid is provided. The method comprises culturing a cell comprising at least one exogenous or overexpressed polynucleotide comprising a nucleic acid sequence encoding a polypeptide that catalyzes at least one of the following reactions: conversion of pyruvate to citramalate; conversion of citramalate to citraconate; conversion of citraconate to β-methyl-D-malate; conversion of β-methyl-D-malate to 2-oxobutanoate; or conversion of threonine to 2-oxobutanoate, under conditions allowing expression of the polynucleotide(s) and production of anteiso fatty acid. Optionally the cell further comprises at least one exogenous or overexpressed polynucleotide comprising a nucleic acid sequence encoding a polypeptide that catalyzes conversion of 2-oxobutanoate to 2-aceto-2-hydroxy-butyrate, conversion of 2-aceto-2-hydroxy-butyrate to 2,3-dihydroxy-3-methylvalerate, and/or conversion of 2,3-dihydroxy-3-methylvalerate to α-keto-3-methylvalerate. A cell that produces anteiso fatty acid and a method of using the cell to produce anteiso fatty acid also are provided.

    摘要翻译： 提供了制备异头物脂肪酸的方法。 该方法包括培养包含至少一种外源或过表达多核苷酸的细胞，所述多核苷酸包含编码多肽的核酸序列，所述核酸序列催化至少一个以下反应：将丙酮酸转化成柠檬酸盐; 柠檬酸盐向柠康酸盐的转化; 柠檬酸酯转化为β-马来酸甲酯; 将β-马来酸甲酯转化为2-氧代丁酸酯; 或在允许表达多核苷酸和生产异构脂肪酸的条件下将苏氨酸转化为2-氧代丁酸酯。 任选地，细胞还包含至少一种外源或过表达的多核苷酸，其包含编码催化2-氧代丁酸酯转化为2-乙酰-2-羟基丁酸的多肽的核酸序列，将2-乙酰-2-羟基 - 丁酸酯转化为 2,3-二羟基-3-甲基戊酸酯，和/或2,3-二羟基-3-甲基戊酸酯转化为α-酮-3-甲基戊酸酯。 还提供了产生异头物脂肪酸的细胞和使用该细胞产生异头物脂肪酸的方法。

    NOVEL METABOLIC PATHWAY FOR PRODUCING ITACONIC ACID AND METHOD FOR PRODUCING ITACONIC ACID USING SAME
    6.
    发明公开
    NOVEL METABOLIC PATHWAY FOR PRODUCING ITACONIC ACID AND METHOD FOR PRODUCING ITACONIC ACID USING SAME 审中-公开

    公开(公告)号：US20240287551A1

    公开(公告)日：2024-08-29

    申请号：US18572361

    申请日：2022-02-28

    申请人： POSTECH RESEARCH AND BUSINESS DEVELOPMENT FOUNDATION

    发明人： Gyoo Yeol JUNG ,  Dae Yeol YE ,  Myung Hyun NOH ,  Myeong Won MIN

    IPC分类号： C12P7/44 ,  C12N9/10 ,  C12N9/90 ,  C12N15/70

    CPC分类号： C12P7/44 ,  C12N9/1029 ,  C12N9/90 ,  C12N15/70 ,  C12Y203/01182 ,  C12Y503/03006

    摘要： The present invention relates to a novel metabolic pathway for producing itaconic acid and a recombinant microorganism, for producing itaconic acid, into which the metabolic pathway is introduced. It has been confirmed that the recombinant microorganism, for producing itaconic acid, into which the novel itaconic acid metabolic pathway is introduced, according to the present invention, significantly increases the production and yield of itaconic acid. In addition, as the recombinant microorganism for producing itaconic acid of the present invention uses an intermediate material of glycolysis rather than an intermediate material of the TCA cycle, the recombinant microorganism can further increase the production yield of itaconic acid through further improvement. Accordingly, the novel metabolic pathway for producing itaconic acid and the recombinant microorganism into which the metabolic pathway is introduced can increase the economic feasibility of itaconic acid, and thus can be variously used in industrial fields for synthetic resins, latexes, food additives, and the like in which itaconic acid is utilized.