专利检索 ap:("UNIVERSITY OF GEORGIA RESEARCH FOUNDATION, INC.") AND inv:"Mark Eiteman" 第 1 页

1.

发明授权
Genetically engineered microbes and methods for producing citramalate 有权

公开(公告)号：US10731185B2

公开(公告)日：2020-08-04

申请号：US16086883

申请日：2017-03-21

申请人： UNIVERSITY OF GEORGIA RESEARCH FOUNDATION, INC.

发明人： Xianghao Wu , Mark Eiteman

IPC分类号： C12P7/46 , C12N9/10 , C12N15/70

摘要： Provided herein is a genetically engineered microbe which accumulates citramalate. In one embodiment, the microbe includes an exogenous polynucleotide encoding a citramalate synthase which catalyzes the condensation of acetyl CoA and pyruvic acid. Optionally, the microbe also includes a second exogenous polynucleotide encoding a citrate synthase which catalyzes the condensation of acetyl CoA and oxaloacetate, and the citrate synthase activity in the microbe is reduced compared to a control microbe. In one embodiment, the citrate synthase includes at least one amino acid substitution in the acetyl-CoA binding pocket, the mobile loop, the NADH binding site, and the oxaloacetate binding site, or a combination thereof. Also provided herein are methods for using the genetically engineered microbe, including a method for producing citramalate. The method can further include isolating the citramalate.

2.

发明申请
GENETICALLY ENGINEERED MICROBES AND METHODS FOR PRODUCING CITRAMALATE 审中-公开

公开(公告)号：US20190093135A1

公开(公告)日：2019-03-28

申请号：US16086883

申请日：2017-03-21

申请人： UNIVERSITY OF GEORGIA RESEARCH FOUNDATION, INC.

发明人： Xianghao Wu , Mark Eiteman

IPC分类号： C12P7/46 , C12N9/10

CPC分类号： C12P7/46 , C12N9/10 , C12N9/1025 , C12N9/1029 , C12N15/70 , C12Y203/01182 , C12Y203/03001

摘要： Provided herein is a genetically engineered microbe which accumulates citramalate. In one embodiment, the microbe includes an exogenous polynucleotide encoding a citramalate synthase which catalyzes the condensation of acetyl CoA and pyruvic acid. Optionally, the microbe also includes a second exogenous polynucleotide encoding a citrate synthase which catalyzes the condensation of acetyl CoA and oxaloacetate, and the citrate synthase activity in the microbe is reduced compared to a control microbe. In one embodiment, the citrate synthase includes at least one amino acid substitution in the acetyl-CoA binding pocket, the mobile loop, the NADH binding site, and the oxaloacetate binding site, or a combination thereof. Also provided herein are methods for using the genetically engineered microbe, including a method for producing citramalate. The method can further include isolating the citramalate.