公开(公告)号：US11708589B2

公开(公告)日：2023-07-25

申请号：US16894600

申请日：2020-06-05

申请人： Genomatica, Inc.

发明人： Anthony P. Burgard ,  Mark J. Burk ,  Robin E. Osterhout ,  Jun Sun ,  Priti Pharkya

IPC分类号： C12P7/18 ,  C12N15/52

CPC分类号： C12P7/18 ,  C12N15/52

摘要： Provided herein is a non-naturally occurring microbial organism having a 1,3-butanediol (1,3-BDO) pathway and comprising at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO. In some embodiments, the pathway includes reducing equivalents from CO or hydrogen. In certain embodiments, a 1,3-BDO pathway proceeds by way of central metabolites pyruvate, succinate or alpha-ketoglutarate. Also provided herein is a method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO.

公开(公告)号：US20230136909A1

公开(公告)日：2023-05-04

申请号：US17833695

申请日：2022-06-06

申请人： Genomatica, Inc.

发明人： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC分类号： C12N15/52 ,  C12P7/42 ,  C08F120/06 ,  C08G63/664 ,  C12N15/63 ,  C12P7/24 ,  C12P7/40 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C12P7/62

摘要： Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 3-hydroxyisobutyrate or MAA. Also provided herein are methods for using such an organism to produce 3-hydroxyisobutyrate or MAA.

公开(公告)号：US11535874B2

公开(公告)日：2022-12-27

申请号：US14436722

申请日：2013-10-21

申请人： Genomatica, Inc.

发明人： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC分类号： C12N9/96 ,  C12P7/46 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C07C55/10 ,  C08G63/00 ,  C12N15/70 ,  C12N15/81 ,  C12P7/24 ,  C07C59/235 ,  C12N15/74 ,  C12N15/77

摘要： Provided herein is a non-naturally occurring microbial organism (NNOMO) having a methanol metabolic pathway (MMP) that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as succinate. Also provided herein are methods for using such an organism to produce succinate.

公开(公告)号：US20220348932A1

公开(公告)日：2022-11-03

申请号：US17516424

申请日：2021-11-01

申请人： Genomatica, Inc.

发明人： Robin E. Osterhout ,  Anthony P. Burgard ,  Priti Pharkya ,  Stefan Andrae

IPC分类号： C12N15/52 ,  C12N9/88 ,  C12N9/90 ,  C12N9/04 ,  C12N9/10 ,  C12P7/04

摘要： The invention provides non-naturally occurring microbial organisms having a formaldehyde fixation pathway, a formate assimilation pathway, and/or a methanol metabolic pathway in combination with a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway, wherein the microbial organisms selectively produce a fatty alcohol, fatty aldehyde or fatty acid of a specified length or isopropanol. The microbial organisms provided advantageously enhance the production of substrates and/or pathway intermediates for the production of chain length specific fatty alcohols, fatty aldehydes, fatty acids or isopropanol. In some aspects, the microbial organisms of the invention have select gene disruptions or enzyme attenuations that increase production of fatty alcohols, fatty aldehydes or fatty acids. The invention additionally provides methods of using the above microbial organisms to produce a fatty alcohol, a fatty aldehyde, a fatty acid or isopropanol.

公开(公告)号：US11447804B2

公开(公告)日：2022-09-20

申请号：US16178432

申请日：2018-11-01

申请人： Genomatica, Inc.

发明人： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC分类号： C12P17/10 ,  C12P7/44 ,  C12P7/24 ,  C12P13/00 ,  C12N9/04 ,  C08G63/78 ,  C08G73/02 ,  C08G69/16 ,  C08G69/08 ,  A23L29/00 ,  C12N15/52

摘要： Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as adipate, 6-aminocaproate, hexamethylenediamine or caprolactam. Also provided herein are methods for using such an organism to produce adipate, 6-aminocaproate, hexamethylenediamine or caprolactam.

公开(公告)号：US20210238609A1

公开(公告)日：2021-08-05

申请号：US17008243

申请日：2020-08-31

申请人： Genomatica, Inc.

发明人： Anthony P. Burgard ,  Robin E. Osterhout ,  Priti Pharkya ,  Stefan Andrae ,  Ewa Teresa Lis ,  Carla Risso ,  John Douglas Trawick

IPC分类号： C12N15/63 ,  C12P5/02 ,  C12P7/04 ,  C12P7/18 ,  C12N9/88 ,  C07K14/195 ,  C12N15/52 ,  C12N9/10 ,  C12N9/90 ,  C07C1/24 ,  C07C11/167 ,  C07C31/20 ,  C07C33/025 ,  C09D147/00 ,  C09J147/00

摘要： Provided herein are non-naturally occurring microbial organisms having a FaldFP, a FAP and/or metabolic modifications which can further include a MMP, a MOP, a hydrogenase and/or a CODH. These microbial organisms can further include a butadiene, 13BDO, CrotOH, MVC or 3-buten-1-ol pathway. Additionally provided are methods of using such microbial organisms to produce butadiene, 13BDO, CrotOH, MVC or 3-buten-1-ol.

公开(公告)号：US20190300919A1

公开(公告)日：2019-10-03

申请号：US16178432

申请日：2018-11-01

申请人： Genomatica, Inc.

发明人： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC分类号： C12P17/10 ,  C12P13/00 ,  A23L29/00 ,  C12N15/52 ,  C08G69/08 ,  C08G69/16 ,  C12P7/24 ,  C08G63/78 ,  C12P7/44 ,  C08G73/02 ,  C12N9/04

摘要： Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as adipate, 6-aminocaproate, hexamethylenediamine or caprolactam. Also provided herein are methods for using such an organism to produce adipate, 6-aminocaproate, hexamethylenediamine or caprolactam.

公开(公告)号：US10415042B2

公开(公告)日：2019-09-17

申请号：US15184940

申请日：2016-06-16

申请人： Genomatica, Inc.

发明人： Anthony P. Burgard ,  Priti Pharkya ,  Robin E. Osterhout

IPC分类号： C12N1/21 ,  C12N15/52 ,  C12N9/04 ,  C12N9/02 ,  C12N9/10 ,  C12N9/80 ,  C12N9/88 ,  C12P13/00 ,  C12P7/44 ,  C12P13/02 ,  C12P17/10 ,  C12P7/46 ,  C12P7/62

摘要： The invention provides a non-naturally occurring microbial organism having an adipate, 6-aminocaproic acid or caprolactam pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective adipate, 6-aminocaproic acid or caprolactam pathway. The invention additionally provides a method for producing adipate, 6-aminocaproic acid or caprolactam. The method can include culturing an adipate, 6-aminocaproic acid or caprolactam producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an adipate, 6-aminocaproic acid or caprolactam pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce adipate, 6-aminocaproic acid or caprolactam.

公开(公告)号：US20190152887A1

公开(公告)日：2019-05-23

申请号：US16110973

申请日：2018-08-23

申请人： Genomatica, Inc.

发明人： Mark J. Burk ,  Priti Pharkya ,  Stephen J. Van Dien ,  Anthony P. Burgard ,  Christophe H. Schilling

IPC分类号： C07C51/353 ,  C07C67/32 ,  C12P7/62 ,  C12P7/46 ,  C07C67/333 ,  C12P7/40 ,  C07C57/04 ,  C07C51/38 ,  C07C67/08

摘要： The invention provides a method of producing acrylic acid. The method includes contacting fumaric acid with a sufficient amount of ethylene in the presence of a cross-metathesis transformation catalyst to produce about two moles of acrylic acid per mole of fumaric acid. Also provided is an acrylate ester. The method includes contacting fumarate diester with a sufficient amount of ethylene in the presence of a cross-metathesis transformation catalyst to produce about two moles of acrylate ester per mole of fumarate diester. An integrated process for process for producing acrylic acid or acrylate ester is provided which couples bioproduction of fumaric acid with metathesis transformation. An acrylic acid and an acrylate ester production also is provided.

公开(公告)号：US20190085363A1

公开(公告)日：2019-03-21

申请号：US15960410

申请日：2018-04-23

申请人： Genomatica, Inc.

发明人： Anthony P. Burgard ,  Mark J. Burk ,  Robin E. Osterhout ,  Jun Sun ,  Priti Pharkya

IPC分类号： C12P7/18 ,  C12N15/52

摘要： Provided herein is a non-naturally occurring microbial organism having a 1,3-butanediol (1,3-BDO) pathway and comprising at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO. In some embodiments, the pathway includes reducing equivalents from CO or hydrogen. In certain embodiments, a 1,3-BDO pathway proceeds by way of central metabolites pyruvate, succinate or alpha-ketoglutarate. Also provided herein is a method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO.