公开(公告)号：US20170252418A1

公开(公告)日：2017-09-07

申请号：US15510933

申请日：2015-09-16

申请人： HUMBOLDT-UNIVERSITÄT ZU BERLIN ,  UNIVERSITEIT UTRECHT HOLDING B.V.

发明人： Nishith GUPTA ,  Ruben ARROYO-OLARTE ,  Richard LUCIUS ,  Jos BROUWERS ,  J. Bernd HELMS ,  Ildiko DUNAY

IPC分类号： A61K39/012 ,  C12N1/36 ,  C12N1/10 ,  C07F9/10 ,  C12N9/12

CPC分类号： A61K39/012 ,  A61K2039/522 ,  A61K2039/552 ,  C07F9/106 ,  C12N1/10 ,  C12N1/36 ,  C12N9/1288

摘要： A genetically modified coccidian parasites wherein expression of phosphatidylthreonine synthase (PTS) is disrupted, a polynucleotide including a nucleotide sequence encoding a phosphatidylthreonine synthase (PTS) enzyme, which catalyzes the production of a lipid, phosphatidylthreonine (PtdThr). PtdThr is an exclusive, major and physiologically important lipid in selected coccidian parasites, which is required for a normal growth and virulence of coccidian parasites. Coccidian parasites, having the expression of PTS disrupted as described herein, are useful as vaccines. The phosphatidylthreonine synthase enzyme and the nucleotide encoding sequences thereof as well as the phosphatidylthreonine phospholipid can find use in diagnostic methods and diagnostic kits or in vaccine and drug development applications.