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    MICROBIOLOGICAL PRODUCTION OF SHORT FATTY ACIDS AND USES THEREOF
    6.
    发明申请
    MICROBIOLOGICAL PRODUCTION OF SHORT FATTY ACIDS AND USES THEREOF 审中-公开

    公开(公告)号：US20180066240A1

    公开(公告)日：2018-03-08

    申请号：US15561201

    申请日：2016-03-31

    Applicant: Johann Wolfgang Goethe-Universität Frankfurt am Main

    Inventor： Martin Grininger ,  Jan Gajewski ,  Eckhard Boles ,  Renata Pavlovic

    IPC: C12N9/10 ,  C12N15/82 ,  C12P7/04 ,  C12P7/64

    CPC classification number: C12N9/1029 ,  C12N15/82 ,  C12P7/04 ,  C12P7/6409 ,  C12P7/6436 ,  C12P7/649 ,  Y02E50/13

    Abstract: The present invention relates to proteins involved in fatty acid synthesis, such as fatty acid synthases (FAS) variants, comprising one or more polypeptide chains, wherein said polypeptide chain(s) comprise one or more subunits comprising a malonyl/palmitoyl transferase domain (MPT domain), acetyl transferase domain (AT domain), and ketoacyl synthase domain (KS domain), and at least one amino acid substitution in the MPT domain at a position corresponding to R130, in the AT domain at a position corresponding to I306, and/or in the KS domain, preferably in the acyl binding channel and/or at KS domain binding site to ACP, to modulate affinities of acyl intermediates, and optionally further amino acid substitution(s). The present invention relates to the respective polypeptide domains. The present invention further relates to nucleic acid molecules encoding the proteins (or the polypeptide domains) and to host cells containing said nucleic acid molecules. The present invention further relates to a method for the production of short fatty acids, CoA esters of short fatty acids, ethyl esters of short fatty acids, esters of short fatty acids with other metabolites, and/or enzyme bound short fatty acids (C6 to C12), comprising the expression of said nucleic acid molecules, preferably in said host cells. The present invention further relates to a method for the production of biofuels, flavoring compounds and/or fine chemicals, comprising the expression of said nucleic acid molecules, preferably in said host cells. The present invention also relates to the use of the proteins, nucleic acids molecule or host cells for the bulk production of short fatty acids (C6 to C12), the specific production of C6 fatty acids and/or C8 fatty acids, the bulk production of CoA esters of short fatty acids (C6 to C12), the specific production of C6-CoA esters and/or C8-CoA esters, the bulk production of ethyl esters of short fatty acids (C6 to C12), the specific production of C6 fatty acid ethyl esters and/or C8 fatty acid ethyl esters, the bulk production of esters of short fatty acids (C6 to C12) with other metabolites, the specific production of C6 fatty acid esters with other metabolites and/or C8 fatty acid esters with other metabolites, the bulk production of enzyme bound short fatty acids (C6 to C12), the specific production of enzyme bound C6 fatty acids and/or enzyme bound C8 fatty acids, the production of biofuels, fine chemicals and/or flavoring substances.

    Modified photosynthetic microorganisms for producing lipids
    10.
    发明授权
    Modified photosynthetic microorganisms for producing lipids 有权
    用于生产脂质的改良光合微生物

    公开(公告)号：US09523096B2

    公开(公告)日：2016-12-20

    申请号：US13995925

    申请日：2011-12-19

    Applicant: James Roberts ,  Fred Cross ,  Margaret Mary McCormick ,  Ernesto Javier Munoz ,  Brett K. Kaiser ,  Michael Carleton

    Inventor： James Roberts ,  Fred Cross ,  Margaret Mary McCormick ,  Ernesto Javier Munoz ,  Brett K. Kaiser ,  Michael Carleton

    IPC: C12N1/00 ,  C12N1/21 ,  C12N15/82 ,  C07K14/195 ,  C12N9/10 ,  C12N9/12 ,  C12N9/16 ,  C12N9/20 ,  C12N9/90 ,  C12N9/00 ,  C12P7/64

    CPC classification number: C12N15/82 ,  C07K14/195 ,  C12N9/1029 ,  C12N9/12 ,  C12N9/16 ,  C12N9/20 ,  C12N9/90 ,  C12N9/93 ,  C12P7/64 ,  Y02P20/52

    Abstract: This disclosure describes genetically modified photosynthetic microorganisms, e.g., Cyanobacteria, that overexpress an acyl carrier protein (ACP), an acyl-ACP synthase (Aas), or both, optionally in combination with one or more overexpressed or exogenous lipid biosynthesis proteins, and/or one or more overexpressed or exogenous glycogen breakdown proteins. Exemplary biosynthesis proteins include diacyglycerol acyltransferases, thioesterases, phosphatidate phosphatases, phospholipases, triacylglycerol (TAG) hydrolases, fatty acyl-CoA synthetases, and/or acetyl-CoA carboxylases, including combinations thereof. Also included are photosynthetic microorganisms comprising mutations or deletions in a glycogen biosynthesis or storage pathway, which accumulate a reduced amount of glycogen under reduced nitrogen conditions as compared to a wild type photosynthetic microorganism. The modified photosynthetic microorganisms provided herein are capable of producing increased amounts of lipids such as fatty acids and/or synthesizing triglycerides.

    Abstract translation: 本公开描述了任选与一种或多种过表达或外源脂质生物合成蛋白组合的转基因修饰的光合微生物，例如过度表达酰基载体蛋白（ACP），酰基-ACP合酶（Aas）或两者的蓝细菌，和/ 或一种或多种过表达或外源糖原分解蛋白。 示例性生物合成蛋白包括二酰甘油酰基转移酶，硫酯酶，磷脂酸磷酸酶，磷脂酶，三酰基甘油（TAG）水解酶，脂肪酰辅酶A合成酶和/或乙酰辅酶A羧化酶，包括其组合。 还包括光合微生物，其包含糖原生物合成或储存途径中的突变或缺失，与野生型光合微生物相比，其在降低的氮条件下积累减少量的糖原。 本文提供的修饰的光合微生物能够产生增加量的脂质，例如脂肪酸和/或合成甘油三酯。

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