公开(公告)号：US20240294899A1

公开(公告)日：2024-09-05

申请号：US18432527

申请日：2024-02-05

Applicant: Iontas Limited

Inventor： John MCCAFFERTY ,  Michael DYSON ,  Kothai PARTHIBAN

IPC: C12N15/10 ,  C07K16/00 ,  C12N15/90

CPC classification number: C12N15/102 ,  C07K16/005 ,  C12N15/1037 ,  C12N15/907 ,  C07K2317/24 ,  C07K2317/622 ,  C07K2317/64 ,  C07K2319/03 ,  C07K2319/035 ,  C07K2319/036 ,  C12N2310/20 ,  C12N2800/30 ,  C12N2800/80

Abstract: The invention relates to methods of producing eukaryotic cell libraries encoding a repertoire of binding molecules (“binders”), wherein the methods use a site-specific nuclease for targeted cleavage of cellular DNA to enhance site-specific integration of binder genes through endogenous cellular repair mechanisms. Populations of eukaryotic cells are produced in which a repertoire of genes encoding binders are integrated into a desired locus in cellular DNA (e.g., a genomic locus) allowing expression of the encoded binding molecule, thereby creating a population of cells expressing different binders.

公开(公告)号：US20240277766A1

公开(公告)日：2024-08-22

申请号：US18541684

申请日：2023-12-15

Applicant: Senti Biosciences, Inc.

Inventor： Marcela GUZMAN AYALA ,  Russell Morrison GORDLEY ,  Michelle Elizabeth HUNG ,  Gary LEE ,  Timothy Kuan-Ta LU

IPC: A61K35/17 ,  A61K9/00 ,  A61K39/00 ,  A61P35/00 ,  C07K14/54 ,  C07K16/30 ,  C12N5/0783

CPC classification number: A61K35/17 ,  A61K9/0019 ,  A61K39/4613 ,  A61K39/4631 ,  A61K39/464402 ,  A61P35/00 ,  C07K14/5434 ,  C07K14/5443 ,  C07K16/303 ,  C12N5/0646 ,  A61K2239/13 ,  A61K2239/21 ,  C07K2317/622 ,  C07K2319/02 ,  C07K2319/036 ,  C07K2319/50 ,  C12N2510/00

Abstract: Described herein are immunoresponsive cells engineered to express cytokines, chimeric receptors, and synthetic transcription factor systems. Also described herein are nucleic acids, cells, and methods directed to the same.

公开(公告)号：US20240190925A1

公开(公告)日：2024-06-13

申请号：US18286835

申请日：2022-04-05

Applicant: KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY

Inventor： Dae Hee LEE ,  Seung Goo LEE ,  Tae Hyun KIM ,  Seong Keun KIM ,  Min Jeong CHOI ,  Seung Gyun WOO ,  Hyun Seung LIM ,  Seokjin OH

IPC: C07K14/195 ,  C12N15/70 ,  C12N15/74

CPC classification number: C07K14/195 ,  C12N15/70 ,  C12N15/74 ,  C07K2319/02 ,  C07K2319/036

Abstract: The present invention relates to a novel signal sequence peptide and a vector comprising a polynucleotide encoding same. The signal sequence peptide can induce a protein linked thereto to be secreted to the outside of a cell, and thus, when the signal sequence peptide is used or a recombinant microorganism transformed with the vector is used, the signal sequence peptide expresses a target protein and then secretes the target protein to the outside of a cell, thus enabling the target protein to exhibit the activity and function thereof.

公开(公告)号：US11723932B2

公开(公告)日：2023-08-15

申请号：US16069220

申请日：2017-01-11

Applicant: Synlogic Operating Company, Inc.

Inventor： Dean Falb ,  Jonathan W. Kotula ,  Vincent M. Isabella ,  Paul F. Miller ,  Suman Machinani ,  Saurabh Saha ,  Adam B. Fisher ,  Yves Millet ,  Ning Li

IPC: A01N63/00 ,  A61K35/74 ,  C07K16/28 ,  A61P35/00 ,  C07K14/54 ,  C12N15/70 ,  C12P13/22 ,  A61K38/20 ,  C12N15/00 ,  C12N5/00 ,  C07K14/34 ,  A61K31/00 ,  C07K14/335 ,  C07K14/245 ,  A61K39/395 ,  C07K14/535 ,  C07K14/55 ,  A61K48/00 ,  A61K38/19 ,  C12P21/02 ,  A61K39/00

CPC classification number: A61K35/74 ,  A61K31/00 ,  A61K38/193 ,  A61K38/20 ,  A61K39/3955 ,  A61K39/39541 ,  A61K48/00 ,  A61P35/00 ,  C07K14/245 ,  C07K14/335 ,  C07K14/34 ,  C07K14/535 ,  C07K14/54 ,  C07K14/5434 ,  C07K14/5443 ,  C07K14/55 ,  C07K16/2803 ,  C07K16/2818 ,  C07K16/2827 ,  C12N5/00 ,  C12N15/00 ,  C12N15/70 ,  C12P13/227 ,  C12P21/02 ,  A61K2039/505 ,  C07K2317/622 ,  C07K2317/76 ,  C07K2319/03 ,  C07K2319/036 ,  C07K2319/40 ,  A61K39/39541 ,  A61K2300/00 ,  A61K39/3955 ,  A61K2300/00

Abstract: Genetically programmed microorganisms, such as bacteria or virus, pharmaceutical compositions thereof, and methods of modulating and treating cancers are disclosed.

公开(公告)号：US20190248851A1

公开(公告)日：2019-08-15

申请号：US16283289

申请日：2019-02-22

Applicant: The Regents of the University of California

Inventor： Kang TING ,  Chia SOO

IPC: C07K14/475 ,  C12N15/62 ,  C07K14/51

CPC classification number: C07K14/475 ,  C07K14/51 ,  C07K2319/02 ,  C07K2319/036 ,  C12N15/625

Abstract: The present invention provides a method and system for producing a NELL protein. The method and system comprise a CELL encoding a NELL protein or peptide and a non-insect secretory signal peptide.

公开(公告)号：US09957307B2

公开(公告)日：2018-05-01

申请号：US14448881

申请日：2014-07-31

Applicant: F. Hoffmann-La Roche AG

Inventor： Jeremy Beauchamp ,  Anita Dreyer ,  Hugues Matile

IPC: C07K14/445 ,  C12N15/85 ,  C07K16/20 ,  G01N33/569

CPC classification number: C07K14/445 ,  C07K16/205 ,  C07K2317/14 ,  C07K2319/02 ,  C07K2319/03 ,  C07K2319/036 ,  C07K2319/21 ,  C07K2319/40 ,  C07K2319/43 ,  C12N15/85 ,  C12N2810/855 ,  G01N33/56905

Abstract: The present invention provides expression vectors for cell-surface expression of polypeptides comprising a transmembrane domain of glycophorin A.

公开(公告)号：US09796990B2

公开(公告)日：2017-10-24

申请号：US14233878

申请日：2012-07-20

Applicant: Colin Scott ,  John Oakeshott ,  Robyn Russell ,  Nigel French ,  Steven Kotsonis ,  Kaiyan Liu

Inventor： Colin Scott ,  John Oakeshott ,  Robyn Russell ,  Nigel French ,  Steven Kotsonis ,  Kaiyan Liu

IPC: C12N9/16 ,  C12P9/00 ,  C12Q1/44 ,  C12Q1/00 ,  C12Q1/42 ,  A62D3/02 ,  A61K38/00 ,  A62D101/04 ,  A62D101/26

CPC classification number: C12P9/00 ,  A61K38/00 ,  A62D3/02 ,  A62D2101/04 ,  A62D2101/26 ,  C07K2319/00 ,  C07K2319/036 ,  C12N9/16 ,  C12Q1/005 ,  C12Q1/42 ,  C12Q1/44 ,  C12Y301/08001 ,  C12Y301/08002 ,  G01N2430/10

Abstract: The present invention relates to enzymes capable of hydrolysing organophosphate (OP) molecules. In particular, the invention relates to variants of the OpdA enzyme from Agrobacterium that display improved activity when compared to the naturally occurring OpdA. The invention is also towards polypeptides that have organophosphate hydrolysing activity for the organophosphates chlorpyrifos methyl, diazinon and parathion ethyl.

公开(公告)号：US09598480B2

公开(公告)日：2017-03-21

申请号：US13769222

申请日：2013-02-15

Applicant: The Regents of the University of California

Inventor： Kang Ting ,  Chia Soo

IPC: C12N15/06 ,  C12N15/07 ,  C12N15/12 ,  C12N15/19 ,  C12N15/63 ,  C12N15/85 ,  C07K14/475 ,  C07K14/51 ,  C12N15/62

CPC classification number: C07K14/475 ,  C07K14/51 ,  C07K2319/02 ,  C07K2319/036 ,  C12N15/625

Abstract: The present invention provides a method and system for producing a NELL protein. The method and system comprise a CELL encoding a NELL protein or peptide and a non-insect secretory signal peptide.

公开(公告)号：US20170058018A2

公开(公告)日：2017-03-02

申请号：US14476559

申请日：2014-09-03

Applicant: Stephen C. Brown ,  Michael R. Simon

Inventor： Stephen C. Brown ,  Michael R. Simon

IPC: C07K16/00

CPC classification number: C07K16/00 ,  A61K2039/505 ,  B01D15/3804 ,  C07K1/14 ,  C07K14/70535 ,  C07K16/065 ,  C07K16/18 ,  C07K2317/21 ,  C07K2319/036 ,  C07K2319/10 ,  C07K2319/32

Abstract: A process for synthesizing and separating secretory IgA from a mixture of IgA monmer and IgA dimer is provided. The process includes covalently binding affinity tagged or epitope tagged recombinant secretory component to the IgA dimer in the mixture and binding the affinity tagged or an epitope tagged secretory IgA to immobilized moieties on the solid phase support resin to which the affinity tag or epitope tag binds and then eluting the affinity tagged or an epitope tagged secretory IgA with release buffer. A process for synthesizing and separating secretory IgM from a mixture of IgM and other plasma proteins is also provided. The process includes covalently binding affinity tagged or an epitope tagged recombinant secretory component to the IgM in the mixture and binding the affinity tagged or an epitope tagged secretory IgM to immobilized moieties on the solid phase support resin and then eluting the peptide tagged secretory IgM with a release buffer.

Abstract translation: 提供了从IgA单体和IgA二聚体的混合物合成和分离分泌型IgA的方法。 该方法包括将亲和标记或表位标记的重组分泌组分共价结合至混合物中的IgA二聚体，并将亲和标记的或表位标记的分泌型IgA与亲和标签或表位标签结合的固相支持树脂上的固定部分结合，以及 然后用释放缓冲液洗脱亲和标记或表位标记的分泌型IgA。 还提供了从IgM和其他血浆蛋白质的混合物中合成和分离分泌型IgM的方法。 该方法包括将亲和标记或表位标记的重组分泌组分共价结合到混合物中的IgM，并将亲和标记或表位标记的分泌型IgM结合到固相支持树脂上的固定化部分，然后用肽标记的分泌型IgM 释放缓冲液。

公开(公告)号：US20160370353A1

公开(公告)日：2016-12-22

申请号：US15159585

申请日：2016-05-19

Applicant: TECHNISCHE UNIVERSITÄT DRESDEN MEDIZINISCHE FAKULTÄT CARL GUSTAV CARUS

Inventor： Michele Solimena ,  Anna Ivanova

IPC: G01N33/50 ,  A01K67/027

CPC classification number: G01N33/5076 ,  A01K67/0275 ,  A01K2217/07 ,  A01K2227/105 ,  A01K2267/0393 ,  C07K2319/036 ,  C12N15/8509 ,  G01N33/5005 ,  G01N33/74

Abstract: The present invention relates to an in vitro method of detecting secretory granules (SGs) of different ages in a cell by differentially labelling said SGs according to their age comprising (a) contacting a cell capable of forming SGs and expressing 1. a (poly)peptide specific for SGs, wherein said (poly)peptide has a binding site common for at least three different substances A, B and C covalently binding thereto or 2. a fusion protein comprising (i) a (poly)peptide specific for SGs and (ii) a (poly)peptide having a binding site common for at least three different substances A, B and C covalently binding thereto, wherein said substances are capable of penetrating the cell membrane and wherein at least substances A and Care detectable by different means with a substance A targeting said binding site (b) saturating said binding sites by contacting the cell with a substance B which blocks the binding sites which were not bound by substance A, (c) allowing that unbound substance B is removed from the cell or removing unbound substance B from the cell, (d) contacting the cell with a substance C targeting said binding site on the (poly)peptide or the fusion protein becoming available for binding after step (b) and (e) detecting for the presence of both detectable substances A and C, wherein the simultaneous detection of both substances A and C is indicative of the presence of two populations of SGs having a different age. Further, the present invention relates to an in vitro method of investigating in a cell the effect of a stimulus on secretory granules (SGs) differentially labelled according to their age.

Abstract translation: 本发明涉及通过根据其年龄差异标记所述SGs来检测细胞中不同年龄的分泌颗粒（SG）的体外方法，其包括（a）使能够形成SG的细胞接触并表达1.a（聚） 肽的特异性肽，其中所述（多）肽具有与共价结合的至少三种不同物质A，B和C共有的结合位点或2.融合蛋白，其包含（i）特异于SG的（多）肽和（ ii）具有与至少三种与其共价结合的至少三种不同物质A，B和C的结合位点的（多）肽，其中所述物质能够穿透细胞膜，并且其中至少可以通过不同手段检测到的物质A和Care， 靶向所述结合位点的物质A（b）通过使细胞与阻断未被物质A结合的结合位点的物质B接触而饱和所述结合位点，（c）允许未结合物质B被去除 从细胞中除去未结合的物质B或从细胞中除去未结合的物质B，（d）使细胞与在步骤（b）和（e）之后可用于结合的（多）肽或融合蛋白上靶向所述结合位点的物质C接触， 检测两种可检测物质A和C的存在，其中同时检测物质A和C两者指示存在两个具有不同年龄的SG群体。 此外，本发明涉及一种在细胞中调查刺激对根据其年龄进行差异标记的分泌颗粒（SG）的影响的体外方法。