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    Method for isolating and purifying bacteriocin against drug-resistant
    2.
    发明授权
    Method for isolating and purifying bacteriocin against drug-resistant 有权

    公开(公告)号：US11999769B2

    公开(公告)日：2024-06-04

    申请号：US18054546

    申请日：2022-11-11

    申请人： Zhejiang Gongshang University

    发明人： Qing Gu ,  Mengdi Dai ,  Qingqing Zhou ,  Ping Li ,  Danli Wu

    IPC分类号： C12P21/02 ,  C07K14/335

    CPC分类号： C07K14/335 ,  C12P21/02

    摘要： The present disclosure discloses a method for isolating and purifying Lactobacillus rhamnosus ZFM216 bacteriocin, the method including the following steps: preparing L. rhamnosus ZFM216 fermentation supernatant; treating the fermentation supernatant with macroporous adsorption resin method to obtain a crude protein extract; isolating the crude protein extract by gel chromatography to obtain a preliminary purified protein solution; and then performing purification by reversed-phase high performance liquid chromatography (HPLC) to obtain an L. rhamnosus ZFM216 bacteriocin solution. The present disclosure further provides use of the L. rhamnosus bacteriocin in anti-Staphylococcus aureus.

    MUCOADHESIVE MICROORGANISM
    7.
    发明申请
    MUCOADHESIVE MICROORGANISM 有权

    公开(公告)号：US20210070815A1

    公开(公告)日：2021-03-11

    申请号：US17026379

    申请日：2020-09-21

    申请人： INTREXON ACTOBIOTICS N.V.

    发明人： Lothar STEIDLER ,  Klaas VANDENBROUCKE

    IPC分类号： C07K14/335 ,  A61K35/741 ,  C07K14/575 ,  A61P3/10 ,  A61K35/74 ,  C07K14/435 ,  C12N9/88

    摘要： The present disclosure provides genetically modified microorganisms (e.g., bacteria or yeast) with enhanced mucin-binding and/or cell-adhesion properties. For example, the present disclosure provides bacteria exhibiting increased in vitro binding to Caco-2 cells, and increased in vitro binding to mucins. Such microorganisms (e.g., bacteria) can be used, e.g., to deliver bioactive polypeptides to the gastrointestinal tract of a mammalian subject. Modifying the microorganism in the described manner allows for the modulation of gastrointestinal retention and transit times for the microorganism (e.g., bacterium). Exemplary microorganisms (e.g., lactic acid bacteria, such as Lactococcus lactis) contain an exogenous nucleic acid encoding a fusion protein containing a cell-adherence polypeptide, such as CmbA, and a mucin-binding polypeptide, such as a trefoil factor (TFF), e.g., human TFF3. The current disclosure further provides method for making and using the described microorganisms (e.g., bacteria).

    Mucoadhesive microorganism
    9.
    发明授权
    Mucoadhesive microorganism 有权

    公开(公告)号：US10808014B2

    公开(公告)日：2020-10-20

    申请号：US16331660

    申请日：2017-09-11

    申请人： INTREXON ACTOBIOTICS N.V.

    发明人： Lothar Steidler ,  Klaas Vandenbroucke

    IPC分类号： A61K35/74 ,  C07K14/575 ,  C07K14/435 ,  A61K39/00 ,  A61P3/10 ,  C07K14/335 ,  A61K35/741 ,  C12N9/88 ,  A61K35/00

    摘要： The present disclosure provides genetically modified microorganisms (e.g., bacteria or yeast) with enhanced mucin-binding and/or cell-adhesion properties. For example, the present disclosure provides bacteria exhibiting increased in vitro binding to Caco-2 cells, and increased in vitro binding to mucins. Such microorganisms (e.g., bacteria) can be used, e.g., to deliver bioactive polypeptides to the gastrointestinal tract of a mammalian subject. Modifying the microorganism in the described manner allows for the modulation of gastrointestinal retention and transit times for the microorganism (e.g., bacterium). Exemplary microorganisms (e.g., lactic acid bacteria, such as Lactococcus lactis) contain an exogenous nucleic acid encoding a fusion protein containing a cell-adherence polypeptide, such as CmbA, and a mucin-binding polypeptide, such as a trefoil factor (TFF), e.g., human TFF3. The current disclosure further provides method for making and using the described microorganisms (e.g., bacteria).