公开(公告)号：US12234259B2

公开(公告)日：2025-02-25

申请号：US18176631

申请日：2023-03-01

Applicant: Pfizer Inc.

Inventor： Mingming Han ,  Rajesh Kumar Kainthan ,  Jin-Hwan Kim ,  Avvari Krishna Prasad

IPC: C07K1/107 ,  A61K39/09 ,  A61K39/095 ,  A61K47/64 ,  C07H3/06 ,  C07K14/22 ,  C07K14/315 ,  C07K14/34 ,  A61K39/00

Abstract: The present disclosure relates generally to methods of preparing glycoconjugates containing a saccharide conjugated to a carrier protein by use of stable nitroxyl radical related agent/oxidant as an oxidizing agent, to immunogenic compositions comprising such glycoconjugates, and to methods for the use of such glycoconjugates and immunogenic compositions.

公开(公告)号：US12233119B2

公开(公告)日：2025-02-25

申请号：US18467682

申请日：2023-09-14

Applicant: Prime Bio, Inc.

Inventor： Bal Ram Singh

IPC: A61K39/02 ,  A61K39/00 ,  A61K39/08 ,  A61K39/395 ,  A61P31/04 ,  C07K14/19 ,  C07K14/235 ,  C07K14/33 ,  C07K14/34 ,  C12N15/62 ,  C12N15/66 ,  C12N15/70

Abstract: The present invention describes a second-generation tetanus toxoid vaccine and a process for the preparation thereof, comprising the steps of: inducing an E. coli culture OD600=0.5 by adding 0.2 mM IPTG; growing the culture at 14-16° C. for 14 to 20 hours; suspending the culture in 25 mM phosphate buffer containing 200 mM sodium chloride; adding 1% of triton-X-100 to the phosphate buffer, and adding the buffer to the culture; sonicating the culture for a period of 3 minutes (at 5 sec on/off pulse) at 4° C. on cold beads; centrifuging the culture for 60 to 90 minutes; collecting and purifying a supernatant using Ni-NTA affinity column with an eluant; and combining the supernatant into a pool with contaminated bands and concentrating using Centriprep-30 centrifuge filters (30 kDa pores).

公开(公告)号：US20240271169A1

公开(公告)日：2024-08-15

申请号：US18693613

申请日：2022-09-09

Applicant: Evonik Operations GmbH

Inventor： Silke Jerrentrup ,  Brigitte Bathe ,  Kornelia Voß ,  Frank Jankowitsch

IPC: C12P13/08 ,  C07K14/34 ,  C12N9/12

CPC classification number: C12P13/08 ,  C07K14/34 ,  C12N9/1217 ,  C12Y207/02004

Abstract: C. glutamicum strains express modified gluconate repressor proteins GntR1 and GntR2. The C. glutamicum strains have an increased ability to produce L-lysine compared with an ability of a wildtype strain. The gluconate repressor protein GntR1 includes an amino acid sequence of SEQ ID NO: 9, where the amino acid Arg in position 102 is replaced by the amino acid Glu. The gluconate repressor protein GntR2 includes SEQ ID NO: 10, and its activity is attenuated compared to the activity of the GntR2 repressor protein of the wild-type strain. A method is developed for the fermentative production of L-lysine using such C. glutamicum strains.

公开(公告)号：US11970725B2

公开(公告)日：2024-04-30

申请号：US18299479

申请日：2023-04-12

Applicant: SCARAB GENOMICS LLC

Inventor： Frederick R. Blattner ,  Robert E. Novy ,  David A. Frisch ,  Charles Landry ,  Hyunsic Choi ,  Eric A. Steffen ,  John Brandon

IPC: C12N1/21 ,  C07K14/21 ,  C07K14/285 ,  C07K14/34 ,  C12N1/20 ,  C12N9/18 ,  C12P21/06

CPC classification number: C12P21/06 ,  C07K14/21 ,  C07K14/285 ,  C07K14/34 ,  C12N1/20 ,  C12N9/18 ,  C07K2319/02

Abstract: Provided herein are E. coli host strains with improved capacity for producing recombinant proteins.

公开(公告)号：US20240052386A1

公开(公告)日：2024-02-15

申请号：US18260861

申请日：2022-01-11

Applicant: TIANJIN INSTITUTE OF INDUSTRIAL BIOTECHNOLOGY, CHINESE ACADEMY OF SCIENCES

Inventor： Jibin SUN ,  Jiao LIU ,  Ping ZHENG ,  Moshi LIU ,  Yu WANG ,  Wenjuan ZHOU ,  Guannan SUN

IPC: C12P13/24 ,  C07K14/34 ,  C12N1/20 ,  C12N9/12 ,  C12N9/02

CPC classification number: C12P13/24 ,  C07K14/34 ,  C12N1/205 ,  C12N9/1217 ,  C12Y207/02011 ,  C12N9/0071 ,  C12Y114/11002 ,  C12R2001/15

Abstract: A protein having an L-proline efflux function and the use thereof are provided. A method for producing L-proline or hydroxyproline by means of using a protein ThrE is used for producing L-proline by means of enhancing the activity of a polypeptide, having an L-proline efflux function, in an L-proline-producing strain. Alternatively, the method is used for producing hydroxyproline by means of weakening the activity of a polypeptide, having an L-proline efflux function, in L-proline-producing host cells and enhancing the activity of a proline hydroxylase.

公开(公告)号：US20230331788A1

公开(公告)日：2023-10-19

申请号：US18325199

申请日：2023-05-30

Applicant: NATIONAL RESEARCH COUNCIL OF CANADA

Inventor： Luke MASSON ,  Melanie ARBOUR ,  Marie-Anne GAURIAT

IPC: C07K14/34 ,  C07K1/22 ,  C12N15/70 ,  C12N15/90

CPC classification number: C07K14/34 ,  C07K1/22 ,  C12N15/70 ,  C12N15/902 ,  C07K2319/034 ,  C07K2319/55 ,  C12N2500/24 ,  C12N2500/34 ,  C12N2500/35 ,  C12N2500/74

Abstract: An expression system and process for the production of Diphtheria toxin polypeptides or mutated forms thereof, such as the toxoid CRM197 polypeptide, in genetically-modified E. coli with high yield is described. The system and process is based on the uncoupling of biomass growth from recombinant protein induction, i.e. using an inducer of protein production that cannot be used as a carbon source for growth by the bacteria. The use of specific components and conditions that improve protein yields are also described.

公开(公告)号：US20230303718A1

公开(公告)日：2023-09-28

申请号：US18163484

申请日：2023-02-02

Applicant: NYKODE THERAPEUTICS ASA

Inventor： Bjarne BOGEN ,  Agnete Brunsvik FREDRIKSEN ,  Inger SANDLIE

IPC: C07K16/44 ,  C07K16/28 ,  C07K14/005 ,  C07K14/34 ,  C07K14/705 ,  C07K14/715 ,  C12N9/12

CPC classification number: C07K16/44 ,  C07K16/2833 ,  C07K14/005 ,  C07K14/34 ,  C07K14/70578 ,  C07K14/7158 ,  C07K16/28 ,  C12N9/1276 ,  A61K2039/505

Abstract: Recombinant antibody-based molecules that trigger both T-cell and B-cell immune responses are disclosed. The recombinant molecules are comprised by at least one targeting unit and at least one antigenic unit connected through a dimerization motif. Also disclosed are nucleic acid molecules encoding the recombinant antibody-based molecule and methods of treating multiple myeloma or lymphoma in a patient using the recombinant antibody-based molecules or the nucleic acid molecules.

公开(公告)号：US11746130B2

公开(公告)日：2023-09-05

申请号：US17833539

申请日：2022-06-06

Applicant: CJ CHEILJEDANG CORPORATION

Inventor： Jin Ah Rho ,  Byoung Hoon Yoon ,  So-jung Park ,  Min Ji Baek ,  Ji Hye Lee

IPC: C07K14/34 ,  C12N1/20 ,  C12P19/32 ,  C12R1/15

CPC classification number: C07K14/34 ,  C12N1/20 ,  C12N1/205 ,  C12P19/32 ,  C12R2001/15

Abstract: The present disclosure relates to a novel protein variant having an activity of exporting 5′-inosine monophosphate, a microorganism comprising the protein variant, and a method for preparing 5′-inosine monophosphate using the microorganism.

公开(公告)号：US11723932B2

公开(公告)日：2023-08-15

申请号：US16069220

申请日：2017-01-11

Applicant: Synlogic Operating Company, Inc.

Inventor： Dean Falb ,  Jonathan W. Kotula ,  Vincent M. Isabella ,  Paul F. Miller ,  Suman Machinani ,  Saurabh Saha ,  Adam B. Fisher ,  Yves Millet ,  Ning Li

IPC: A01N63/00 ,  A61K35/74 ,  C07K16/28 ,  A61P35/00 ,  C07K14/54 ,  C12N15/70 ,  C12P13/22 ,  A61K38/20 ,  C12N15/00 ,  C12N5/00 ,  C07K14/34 ,  A61K31/00 ,  C07K14/335 ,  C07K14/245 ,  A61K39/395 ,  C07K14/535 ,  C07K14/55 ,  A61K48/00 ,  A61K38/19 ,  C12P21/02 ,  A61K39/00

CPC classification number: A61K35/74 ,  A61K31/00 ,  A61K38/193 ,  A61K38/20 ,  A61K39/3955 ,  A61K39/39541 ,  A61K48/00 ,  A61P35/00 ,  C07K14/245 ,  C07K14/335 ,  C07K14/34 ,  C07K14/535 ,  C07K14/54 ,  C07K14/5434 ,  C07K14/5443 ,  C07K14/55 ,  C07K16/2803 ,  C07K16/2818 ,  C07K16/2827 ,  C12N5/00 ,  C12N15/00 ,  C12N15/70 ,  C12P13/227 ,  C12P21/02 ,  A61K2039/505 ,  C07K2317/622 ,  C07K2317/76 ,  C07K2319/03 ,  C07K2319/036 ,  C07K2319/40 ,  A61K39/39541 ,  A61K2300/00 ,  A61K39/3955 ,  A61K2300/00

Abstract: Genetically programmed microorganisms, such as bacteria or virus, pharmaceutical compositions thereof, and methods of modulating and treating cancers are disclosed.

公开(公告)号：US20230203106A1

公开(公告)日：2023-06-29

申请号：US17778810

申请日：2020-06-10

Applicant: CJ CHEILJEDANG CORPORATION

Inventor： Kyungrim Kim ,  Tae Yeon Kim ,  Imsang Lee ,  Kwang Woo Lee ,  Heeyeong Kim ,  Kwang Soo Shin

IPC: C07K14/34 ,  C07K14/245 ,  C12N1/20

CPC classification number: C07K14/34 ,  C07K14/245 ,  C12N1/205

Abstract: The present disclosure relates to an acetohydroxy acid synthase variant, a polynucleotide encoding the variant, a microorganism including the variant, and a method of producing L-isoleucine using the microorganism.