公开(公告)号：US12233119B2

公开(公告)日：2025-02-25

申请号：US18467682

申请日：2023-09-14

Applicant: Prime Bio, Inc.

Inventor： Bal Ram Singh

IPC: A61K39/02 ,  A61K39/00 ,  A61K39/08 ,  A61K39/395 ,  A61P31/04 ,  C07K14/19 ,  C07K14/235 ,  C07K14/33 ,  C07K14/34 ,  C12N15/62 ,  C12N15/66 ,  C12N15/70

Abstract: The present invention describes a second-generation tetanus toxoid vaccine and a process for the preparation thereof, comprising the steps of: inducing an E. coli culture OD600=0.5 by adding 0.2 mM IPTG; growing the culture at 14-16° C. for 14 to 20 hours; suspending the culture in 25 mM phosphate buffer containing 200 mM sodium chloride; adding 1% of triton-X-100 to the phosphate buffer, and adding the buffer to the culture; sonicating the culture for a period of 3 minutes (at 5 sec on/off pulse) at 4° C. on cold beads; centrifuging the culture for 60 to 90 minutes; collecting and purifying a supernatant using Ni-NTA affinity column with an eluant; and combining the supernatant into a pool with contaminated bands and concentrating using Centriprep-30 centrifuge filters (30 kDa pores).

公开(公告)号：US10407692B2

公开(公告)日：2019-09-10

申请号：US15607498

申请日：2017-05-28

Applicant: Futuragene Israel Ltd.

Inventor： Hanan Stein ,  Dror Avisar

IPC: C12N15/82 ,  C07K14/19 ,  C07K14/195

Abstract: A nucleic acid expression vector comprising a nucleic acid sequence encoding a dominant negative T3SS protein is disclosed. The nucleic acid expression vector further comprising a cis acting regulatory element capable of driving transcription of the nucleic acid sequence in a plant cell. Moreover, the dominant negative T3SS protein mediates assembly of a dysfunctional needle complex.

公开(公告)号：US20190076518A1

公开(公告)日：2019-03-14

申请号：US15064651

申请日：2016-03-09

Applicant: Bal Ram Singh

Inventor： Bal Ram Singh

IPC: A61K39/02 ,  A61K39/08 ,  A61K39/00 ,  C12N15/70 ,  C12N15/62 ,  C12N15/66 ,  C07K14/19 ,  C07K14/34 ,  C07K14/33 ,  C07K14/235 ,  A61K39/395 ,  A61P31/04

Abstract: The present invention describes a neurotoxin associated protein from botulinum neurotoxin complex used as an oral or nasal delivery system for a vaccine. The vaccine is selected from tetanus, diphtheria and pertussis alone or in combination. Further the oral or nasal delivery of tetanus vaccine in combination with other drug molecules.

公开(公告)号：US6033668A

公开(公告)日：2000-03-07

申请号：US344639

申请日：1994-11-14

Applicant: Michel H. Klein ,  Run-Pan Du ,  Mary E. Ewasyshyn

Inventor： Michel H. Klein ,  Run-Pan Du ,  Mary E. Ewasyshyn

IPC: A61K39/00 ,  A61K39/155 ,  A61K39/295 ,  A61P11/00 ,  A61P31/12 ,  A61P31/16 ,  A61P37/04 ,  C07K1/22 ,  C07K14/005 ,  C07K14/11 ,  C07K14/115 ,  C07K14/135 ,  C07K14/19 ,  C07K14/195 ,  C07K14/41 ,  C07K19/00 ,  C12N5/10 ,  C12N15/09 ,  C12N15/10 ,  C12N15/33 ,  C12N15/45 ,  G01N33/53 ,  G01N33/569 ,  A61K39/12 ,  A61K39/385 ,  C07K1/00

CPC classification number: G01N33/56983 ,  C07K14/005 ,  A61K39/00 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/40 ,  C12N2760/18522 ,  C12N2760/18622 ,  G01N2333/115 ,  G01N2333/135

Abstract: Multimeric hybrid genes encoding the corresponding chimeric protein comprise a gene sequence coding for an antigenic region of a protein from a first pathogen linked to a gene sequence coding for an antigenic region of a protein from a second pathogen. The pathogens particularly are parainfluenza virus (PIV) and respiratory syncytial virus (RSV). A single recombinant immunogen is capable of protecting infants and similar susceptible individuals against diseases caused by both PIV and RSV.

Abstract translation: 编码相应嵌合蛋白质的多聚体杂交基因包含编码与编码来自第二病原体的蛋白质的抗原性区域的基因序列连接的第一病原体的蛋白质的抗原性区的基因序列。 病原体特别是副流感病毒（PIV）和呼吸道合胞病毒（RSV）。 单一的重组免疫原能够保护婴儿和类似的敏感个体免受由PIV和RSV引起的疾病。

公开(公告)号：US6017539A

公开(公告)日：2000-01-25

申请号：US852344

申请日：1997-05-07

Applicant: Michel H. Klein ,  Run-Pan Du ,  Mary E. Ewasyshyn

Inventor： Michel H. Klein ,  Run-Pan Du ,  Mary E. Ewasyshyn

IPC: A61K39/00 ,  A61K39/155 ,  A61K39/295 ,  A61P11/00 ,  A61P31/12 ,  A61P31/16 ,  A61P37/04 ,  C07K1/22 ,  C07K14/005 ,  C07K14/11 ,  C07K14/115 ,  C07K14/135 ,  C07K14/19 ,  C07K14/195 ,  C07K14/41 ,  C07K19/00 ,  C12N5/10 ,  C12N15/09 ,  C12N15/10 ,  C12N15/33 ,  C12N15/45 ,  G01N33/53 ,  G01N33/569 ,  A61K39/12 ,  A61K39/385 ,  C07K1/00

CPC classification number: G01N33/56983 ,  C07K14/005 ,  A61K39/00 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/40 ,  C12N2760/18522 ,  C12N2760/18622 ,  G01N2333/115 ,  G01N2333/135

Abstract: Multimeric hybrid genes encoding the corresponding chimeric protein comprise a gene sequence coding for an antigenic region of a protein from a first pathogen linked to a gene sequence coding for an antigenic region of a protein from a second pathogen. The pathogens particularly are parainfluenza virus (PIV) and respiratory syncytial virus (RSV). A single recombinant immunogen is capable of protecting infants and similar susceptible individuals against diseases caused by both PIV and RSV.

公开(公告)号：US09802989B2

公开(公告)日：2017-10-31

申请号：US13819774

申请日：2011-08-30

Applicant: Sammy Boussiba ,  Ehud Peled ,  Uri Pick ,  Aliza Zarka ,  Stefan Lou ,  Meira Weiss

Inventor： Sammy Boussiba ,  Ehud Peled ,  Uri Pick ,  Aliza Zarka ,  Stefan Lou ,  Meira Weiss

IPC: C07K14/40 ,  C07K14/19 ,  C07K14/405 ,  C07K14/195

CPC classification number: C07K14/405 ,  C07K14/195

Abstract: An isolated novel oil globule protein encoded by a polynucleotide sequence is provided together with a composition which includes the isolated protein. A transgenic organism transformed by a polynucleotide encoding a protein which at least partially comprises the amino acid sequence of the novel oil globule protein is also provided. The invention also provides a method for producing or enhancing the production of a carotenoid such as astaxanthin, which is an oil globule constituent.

公开(公告)号：US5556757A

公开(公告)日：1996-09-17

申请号：US482847

申请日：1995-06-07

Applicant: Diane V. Alstyne ,  Lawrence R. Sharma

Inventor： Diane V. Alstyne ,  Lawrence R. Sharma

IPC: A61K38/00 ,  A61K39/00 ,  C07K14/16 ,  C07K14/19 ,  C07K14/195 ,  C07K14/22 ,  C07K14/315 ,  C07K16/10 ,  C07K16/12 ,  C12N5/20 ,  G01N33/53 ,  C07K7/00 ,  G01N33/554 ,  G01N33/569

CPC classification number: C07K14/005 ,  C07K14/195 ,  C07K14/22 ,  C07K14/3156 ,  C07K16/1054 ,  C07K16/1063 ,  C07K16/1081 ,  C07K16/12 ,  A61K38/00 ,  A61K39/00 ,  C12N2740/16122 ,  C12N2740/16222 ,  C12N2770/36222

Abstract: Peptides comprising a Meningitis Related Homologous Antigenic Sequence (MRHAS) are provided. The MRHAS is found in meningitis-causing organisms and chemokines involved in cell chemotaxis. The peptides are useful as antigens and vaccines for detection, diagnosis and treatment of meningitis.

Abstract translation: 提供了包含脑膜炎相关同源抗原序列（MRHAS）的肽。 MRHAS被发现在引起脑膜炎的生物和涉及细胞趋化性的趋化因子。 该肽可用作用于脑膜炎检测，诊断和治疗的抗原和疫苗。

公开(公告)号：US5439814A

公开(公告)日：1995-08-08

申请号：US93453

申请日：1993-07-19

Applicant: Teryl K. Frey ,  Geraldina Dominguez ,  Chin-Yen Wang

Inventor： Teryl K. Frey ,  Geraldina Dominguez ,  Chin-Yen Wang

IPC: A61K39/00 ,  C07K14/19 ,  C12N15/40 ,  C12N15/00 ,  C12N7/00 ,  C12N15/63

CPC classification number: C07K14/005 ,  A61K39/00 ,  C12N2770/36222

Abstract: The entire rubella virus genomic RNA has been sequenced. An infectious cDNA clone has been constructed. Mutants of this clone that are rendered non-pathogenic are used as vaccines to vaccinate humans, including pregnant or older women, with decreased risk of causing fetal infection, autoimmune disease or neurological symptoms.

Abstract translation: 整个风疹病毒基因组RNA已被测序。 已经构建了感染性cDNA克隆。 使用非致病性的这种克隆的突变体用作接种人类（包括怀孕或老年妇女）的疫苗，具有引起胎儿感染，自身免疫疾病或神经症状的风险降低。

公开(公告)号：US5169784A

公开(公告)日：1992-12-08

申请号：US583392

申请日：1990-09-17

Applicant: Max D. Summers ,  Christian E. G. Oker-Blom

Inventor： Max D. Summers ,  Christian E. G. Oker-Blom

IPC: A61K39/00 ,  A61K39/20 ,  C07K14/18 ,  C07K14/19 ,  C12N7/00 ,  C12N15/09 ,  C12N15/40 ,  C12N15/86 ,  C12N15/866

CPC classification number: C12N15/86 ,  C07K14/005 ,  C12N7/00 ,  A61K39/00 ,  C12N2710/14121 ,  C12N2710/14143 ,  C12N2710/14144 ,  C12N2770/36122 ,  C12N2770/36143 ,  C12N2770/36222

Abstract: This invention relates to specifically designing and genetically engineering recombinant baculovirus for producing, in a compatible insect system, a desired protein, virus, protein hybrid, or virus hybrid. In particular aspects, this invention relates to the use of different baculovirus promoters for the ultimate purpose of constructing a recombinant baculovirus designed for the investigator's specific need. For example, the recombinant baculovirus of this invention can be designed to produce a viral pesticide.This invention also describes the construction of a genetically engineered virus or virus hybrid (e.g. animal or human pathogen) which is not capable of replicating itself but is essentially identical to the authentic pathogen in terms of structure and antigenicity. This baculovirus is constructed such that the non-structural viral genes are truncated, mutated or both and are located 3' and directly under the control of an early baculovirus gene promoter and the structural viral genes are located 3' and directly under the control of a late baculovirus gene promoter. This genetically engineered baculovirus is therefore capable of temporal regulation and successive synthesis of non-structural and structural proteins. The truncated or mutated non-structural viral genes creates the non-replicative aspect of this invention. Since the genetically produced virus or virus hybrid is essentially identical to the authentic pathogen, the product is thereby highly antigenic and potent in terms of efficacy and efficiency. This invention enables the design and constructure of a virus particle or virus hybrid with specific antigenic properties which further allows for the safe and inexpensive production of vaccines or diagnostics.

Abstract translation: 本发明涉及专门设计和遗传工程重组杆状病毒，用于在相容的昆虫系统中产生所需的蛋白质，病毒，蛋白质杂种或病毒杂交体。 在具体方面，本发明涉及不同杆状病毒启动子用于构建针对研究者特定需要而设计的重组杆状病毒的最终目的。 例如，本发明的重组杆状病毒可被设计成生产病毒性农药。 本发明还描述了在结构和抗原性方面不能复制自身但与真实病原体基本相同的遗传工程病毒或病毒杂交体（例如动物或人类病原体）的构建。 构建这种杆状病毒使得非结构病毒基因被截短，突变或两者并且位于3'并且直接在早期杆状病毒基因启动子的控制下，并且结构病毒基因位于3'并且直接在a 晚期杆状病毒基因启动子。 因此，这种基因工程的杆状病毒能够进行非结构和结构蛋白质的时间调节和连续合成。 截短或突变的非结构病毒基因产生本发明的非复制方面。 由于遗传产生的病毒或病毒杂合物与真实病原体基本上相同，因此产品在功效和效率方面具有高度的抗原性和有效性。 本发明使得具有特定抗原性质的病毒颗粒或病毒杂交体的设计和结构进一步允许安全和廉价地生产疫苗或诊断。

公开(公告)号：US5164481A

公开(公告)日：1992-11-17

申请号：US397767

申请日：1989-08-23

Applicant: Martial Lacroix ,  Maan Zrein ,  Gervais Dionne

Inventor： Martial Lacroix ,  Maan Zrein ,  Gervais Dionne

IPC: A61K39/00 ,  A61K38/00 ,  A61K39/20 ,  A61K39/395 ,  C07K7/06 ,  C07K7/08 ,  C07K14/00 ,  C07K14/19 ,  C07K16/00 ,  C07K16/10 ,  C12N15/40 ,  C12P21/08 ,  G01N33/569 ,  G01N33/577

CPC classification number: C07K14/005 ,  C07K16/10 ,  A61K38/00 ,  A61K39/00 ,  C12N2770/36222

Abstract: Linear and cyclic peptides of the E1 and C protein of the rubella virus are disclosed. These peptides and analogues, mixtures and combinations of them are useful in detecting and quantifying antibodies raised against the rubella virus. They are also useful in raising antibodies to the rubella virus for use in the diagnosis of and protection against rubella viral infections.

Abstract translation: 公开了风疹病毒的E1和C蛋白的线性和环肽。 这些肽及其类似物，它们的混合物和组合可用于检测和定量针对风疹病毒的抗体。 它们也可用于提高风疹病毒的抗体，以用于诊断和保护风疹病毒感染。