公开(公告)号：US20240263229A1

公开(公告)日：2024-08-08

申请号：US18566360

申请日：2022-12-15

Applicant: Illumina, Inc.

Inventor： Xiaoyu Ma ,  Mathieu Lessard-Viger ,  Jeffrey Fisher ,  Jonathan Boutell

IPC: C12Q1/6874 ,  C12N15/10 ,  C12Q1/42 ,  C12Q1/6834 ,  C12Q1/6844

CPC classification number: C12Q1/6874 ,  C12N15/1068 ,  C12Q1/42 ,  C12Q1/6834 ,  C12Q1/6844 ,  G01N2333/916

Abstract: The present disclosure is generally directed to strategies for template capture and amplification during sequencing.

公开(公告)号：US20240247301A1

公开(公告)日：2024-07-25

申请号：US18530750

申请日：2023-12-06

Applicant: Ohio State Innovation Foundation

Inventor： Venkat Gopalan ,  Eleanor Bashian ,  Pankajavalli Thirugnanasambantham

IPC: C12Q1/42 ,  C12N9/16 ,  C12Q1/54

CPC classification number: C12Q1/42 ,  C12N9/16 ,  C12Q1/54 ,  C12Y301/03023 ,  G01N2333/916 ,  G01N2800/042

Abstract: The present disclosure relates to a diagnostic assay to detect classic inborn galactosemia. The assay comprises detecting defective galactose metabolism using a substrate-specific recombinant phosphatase derived from Salmonella.

公开(公告)号：US20240026419A1

公开(公告)日：2024-01-25

申请号：US18347930

申请日：2023-07-06

Applicant: Astellas Institute for Regenerative Medicine

Inventor： Irina Klimanskaya ,  Roger Gay

IPC: C12Q1/42 ,  G01N33/569 ,  G01N33/50

CPC classification number: C12Q1/42 ,  G01N33/56966 ,  G01N33/5005

Abstract: Methods are provided for detection of a target cell type within a cell population, and compositions are provided comprising cells and an indicator that indicates the number of cells of the target cell type in the cell population. Examples are provided in which these methods are used to detect human embryonic stem cells within a differentiated cell population with exquisite sensitivity. Differentiated cells produced from embryonic stem cells can be characterized by these methods before transplantation into a recipient, thereby providing further assurance of safety.

公开(公告)号：US11795191B2

公开(公告)日：2023-10-24

申请号：US16371646

申请日：2019-04-01

Applicant: Jingyue Ju ,  Shiv Kumar ,  Chuanjuan Tao ,  Minchen Chien ,  James J. Russo ,  John J. Kasianowicz ,  Joseph W. F. Robertson

Inventor： Jingyue Ju ,  Shiv Kumar ,  Chuanjuan Tao ,  Minchen Chien ,  James J. Russo ,  John J. Kasianowicz ,  Joseph W. F. Robertson

IPC: C12Q1/68 ,  C07H19/00 ,  C12Q1/42 ,  C12Q1/48 ,  C12Q1/6869 ,  C07H19/06 ,  C07H19/16 ,  G01N33/487 ,  C12Q1/6883 ,  B82Y5/00

CPC classification number: C07H19/00 ,  C07H19/06 ,  C07H19/16 ,  C12Q1/42 ,  C12Q1/48 ,  C12Q1/6869 ,  C12Q1/6883 ,  G01N33/48721 ,  B82Y5/00 ,  C12Q2600/158 ,  G01N2333/9125 ,  C12Q1/6869 ,  C12Q2521/525 ,  C12Q2521/543 ,  C12Q2537/157 ,  C12Q2565/631 ,  C12Q1/6869 ,  C12Q2521/525 ,  C12Q2521/543 ,  C12Q2535/107 ,  C12Q2565/631 ,  C12Q1/6869 ,  C12Q2565/631

Abstract: This disclosure provides systems and methods for sequencing nucleic acids using nucleotide analogues and translocation of tags from incorporated nucleotide analogues through a nanopore. In aspects, this disclosure is related to composition, method, and system for sequencing a nucleic acid using tag molecules and detection of translocation through a nanopore of tags released from incorporation of the molecule.

公开(公告)号：US20230313264A1

公开(公告)日：2023-10-05

申请号：US18011545

申请日：2021-07-01

Applicant: VISION DIAGNOSTICS, INC.

Inventor： JERRY W. DENNEY

IPC: C12Q1/42

CPC classification number: C12Q1/42

Abstract: A kit comprising two reagents for conducting two different Assays to measure a zinc-activated enzyme in a bodily fluid. The assay results are utilized to obtain a relative zinc-activated enzyme activity value that can be correlated to tissue zinc levels. A more specific embodiment utilizes two paired reagents for conducting an A Assay and a B Assay that measure different alkaline phosphatase (ALP) activities in a urine sample to obtain a Relative Renal Alkaline Phosphatase Activity (RRAPA) value. The RRAPA value can be correlated to zinc levels in renal tissue.

公开(公告)号：US20230296627A1

公开(公告)日：2023-09-21

申请号：US17971081

申请日：2022-10-21

Applicant: CORNELL UNIVERSITY

Inventor： Alexander J.A. TRAVIS ,  Roy COHEN

IPC: G01N33/68 ,  C12Q1/25 ,  C12Q1/32 ,  C12Q1/42 ,  C12Q1/48 ,  C12Q1/66 ,  C12N11/18

CPC classification number: G01N33/6896 ,  C12Q1/25 ,  C12Q1/32 ,  C12Q1/42 ,  C12Q1/485 ,  C12Q1/66 ,  C12N11/18 ,  G01N2800/2871 ,  G01N2800/2814 ,  G01N2800/2821 ,  G01N2800/2835 ,  G01N2800/2857

Abstract: The present invention relates to methods of detecting a neural injury biomarker in a biological sample. The method includes subjecting a biological sample to an assay according to the present invention that produces a measurable signal and detecting the measurable signal. The presence or absence of the measurable signal indicates the presence or absence of the biomarker in the sample. The present invention also relates to methods of determining the state of a subject's neural injury. The present invention also relates to systems and devices useful in carrying out the methods of the present invention.

公开(公告)号：US20230250466A1

公开(公告)日：2023-08-10

申请号：US17920087

申请日：2021-04-22

Applicant: PAUL HARTMANN AG

Inventor： Hans SMOLA ,  Ana JORGE

IPC: C12Q1/42 ,  C12Q1/37 ,  C12Q1/26 ,  B01L3/00

CPC classification number: C12Q1/42 ,  C12Q1/37 ,  C12Q1/26 ,  B01L3/5023 ,  B01L2300/0825 ,  B01L2300/069

Abstract: The present invention relates to a method for assessing a status of a wound of a patient, comprising the steps of i. determining a concentration of one or more inflammatory markers in a sample from the wound, wherein the sample comprises or consists of wound fluid and the one or more inflammatory markers may indicate an inflammation within the wound, ii. determining a concentration of one or more bacterial markers in the sample, wherein the one or more bacterial markers may indicate a colonization of the wound with metabolically active bacteria, iii. assessing the status of the wound based on the concentrations of the one or more inflammatory markers and the one or more bacterial markers, wherein the status of the wound is assessed as being inflamed or not inflamed and as being colonized with metabolically active bacteria or not colonized with metabolically active bacteria. This diagnostic method allows a reliable, specific and detailed assessment of the status of the wound.

公开(公告)号：US20180312900A1

公开(公告)日：2018-11-01

申请号：US15569583

申请日：2016-05-20

Applicant: AGRIVIDA, INC.

Inventor： Xuemei LI ,  R. Michael RAAB

IPC: C12Q1/42

CPC classification number: C12Q1/34 ,  C12Q1/42

Abstract: Methods of increasing extraction of enzymes from animal feed and measuring enzyme activity are described herein.

公开(公告)号：US20180231564A1

公开(公告)日：2018-08-16

申请号：US15750030

申请日：2016-08-08

Applicant: NORTHWESTERN UNIVERSITY

Inventor： Milan Mrksich ,  Eric J. Berns ,  Maria D. Cebezas

IPC: G01N33/68 ,  C12Q1/37 ,  C12Q1/46

CPC classification number: G01N33/6851 ,  C12Q1/00 ,  C12Q1/26 ,  C12Q1/37 ,  C12Q1/42 ,  C12Q1/44 ,  C12Q1/46 ,  C12Q1/48 ,  C12Q1/485

Abstract: The disclosure provides a cell-based, label-free assay compatible with high-throughput screening (HTS) that can report quantitatively on enzyme activities by measuring mass changes of substrates with MALDI-mass spectrometry.

公开(公告)号：US09989531B2

公开(公告)日：2018-06-05

申请号：US14898904

申请日：2014-06-17

Applicant: Chu Sainte-Justine

Inventor： Alain Moreau ,  Marie-Yvonne Akoume Ndong ,  Mohamed Elbakry

IPC: G01N33/50 ,  G01N21/53 ,  C07H21/04 ,  C07K16/18 ,  G01N33/573 ,  G01N33/68 ,  C12N15/113 ,  C12Q1/68 ,  C12Q1/42 ,  C12Q1/48

CPC classification number: G01N33/573 ,  C12N15/1137 ,  C12N2310/14 ,  C12N2320/30 ,  C12Q1/42 ,  C12Q1/485 ,  C12Q1/6883 ,  C12Q2600/118 ,  C12Q2600/158 ,  C12Y207/01068 ,  C12Y301/03048 ,  G01N33/5047 ,  G01N33/5091 ,  G01N33/6893 ,  G01N2333/912 ,  G01N2333/91215 ,  G01N2333/916 ,  G01N2800/10 ,  G01N2800/50 ,  G01N2800/56

Abstract: Methods of stratifying a subject having or at risk for developing adolescent idiopathic scoliosis (AIS) into diagnostically or clinically useful subclasses are provided. The stratification is based on the subject's PTPμ expression and/or activity and/or PIPK1γ expression and/or activity. Also provided are methods of predicting the risk of developing a scoliosis also based on the subject's PTPμ expression and/or activity and/or PIPK1γ expression and/or activity; and methods of increasing GiPCR signaling in cells of a subject in need thereof comprising administering to the subject's cells an effective amount of an inhibitor of PIPK1γ tyrosine phosphorylation; an activator of PIPK1Y tyrosine dephosphorylation; and/or an inhibitor of PIPK1γ expression and/or activity.