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    Gradient elution moving boundary electrophoresis for use with complex samples and detection of toxins
    3.
    发明授权
    Gradient elution moving boundary electrophoresis for use with complex samples and detection of toxins 有权

    公开(公告)号：US11479804B1

    公开(公告)日：2022-10-25

    申请号：US16236697

    申请日：2018-12-31

    Applicant: Applied Research Associates, Inc. ,  Government of the United States of America, as represented by the Secretary of Commerce, The National Institute Of Standards And Technology

    Inventor： Alyssa Catharyn Henry ,  David Judson Ross

    IPC: C12Q1/46 ,  B01L3/00 ,  G01N27/447 ,  C12Q1/527 ,  B01D57/02 ,  G01N30/28

    Abstract: Methods of detecting the presence of toxins in a sample using electrophoretic separations and of performing electrophoretic separation of complex samples are provided. The method of detecting the presence of toxins includes reacting a sample and a substrate with a signaling enzyme which converts the substrate to the product in a reaction medium, introducing a run buffer into a separation channel having an inlet end, selectively introducing at least one of the substrate and the product of the reaction medium into the inlet end of the separation channel, electrophoretically separating the substrate and the product, and determining the rate of conversion of the substrate to the product, wherein a change in the rate of conversion is indicative of the presence of toxins. The method of performing electrophoretic separations of complex samples having charged particulates and oppositely charged analytes comprising introducing a run buffer into a separation channel having an inlet end, selectively introducing the oppositely charged analytes in the complex sample into the separation channel, and electrophoretically separating the charged particulates and the oppositely charged analytes. Additionally, a device for varying with respect to time the bulk flow of a fluid in a separation channel of an electrophoretic device having a buffer reservoir in fluid contact with the separation channel is provided. The device includes a pressure sensor in fluid contact with a buffer reservoir, a high pressure reservoir in selective fluidic communication with the buffer reservoir, a low pressure reservoir in selective fluidic communication with the buffer reservoir and in fluidic communication with the high pressure reservoir, and a pumping device for pumping a gas from the low pressure reservoir to the high pressure reservoir.

    SENSOR, DETECTION METHOD, REAGENT AND KIT
    4.
    发明申请
    SENSOR, DETECTION METHOD, REAGENT AND KIT 有权

    公开(公告)号：US20220081702A1

    公开(公告)日：2022-03-17

    申请号：US17199380

    申请日：2021-03-11

    Applicant: KABUSHIKI KAISHA TOSHIBA

    Inventor： Tatsuro SAITO ,  Yoshiaki SUGIZAKI

    IPC: C12Q1/46

    Abstract: According to one embodiment, a sensor for detecting a target substance in gas a sample includes a target substance uptake unit that brings an acetylcholine aqueous solution into contact with a gas sample to dissolve a target substance in the gas sample into the acetylcholine aqueous solution, a reaction unit that holds acetylcholinesterase and brings the solution delivered from the target substance uptake unit into contact with the acetylcholinesterase, and a detection unit that measures a change in an amount of acetylcholine decomposition product produced in the solution delivered from the reaction unit.

    Rapid tests for the detection of inhibitors of enzymes and human exposure to the same
    9.
    发明授权
    Rapid tests for the detection of inhibitors of enzymes and human exposure to the same 有权

    公开(公告)号：US10458916B2

    公开(公告)日：2019-10-29

    申请号：US14827674

    申请日：2015-08-17

    Applicant: ANP Technologies, Inc.

    Inventor： Yli Remo Vallejo, Jr. ,  David Milunic

    IPC: C12Q1/44 ,  G01N21/64 ,  C12Q1/46

    Abstract: A device and method for the rapid on-site detection of inhibitors of enzymes, such as, acetylcholinesterase is described where the device contains 2 reaction zones containing a reporter enzyme substrate. One reaction zone is for the test sample while the other is for an onboard negative control. Sample and control fluids are preincubated with the enzyme in separate reaction containers, then an aliquot of each reaction mixture is added to designated reaction zones on the test device. A purpose-built reader or an illuminating device, such as, containing an incandescent light source, a diode, a UV light source or any other illumination source that is suitable for the reporter or mere visualization is used to determine the level of reporter.

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