公开(公告)号：US20240336965A1

公开(公告)日：2024-10-10

申请号：US18601772

申请日：2024-03-11

Applicant: THE J. DAVID GLADSTONE INSTITUTES, A TESTAMENTARY TRUST ESTABLISHED UNDER THE WI ,  The Regents of the University of California ,  The Board of Trustees of the Leland Stanford Junior University

Inventor： Vijay Ramani ,  Ke Wu ,  Hani Goodarzi ,  Arjun Scott Nanda ,  Sivakanthan Kasinathan

IPC: C12Q1/6869 ,  C12N15/10 ,  C12Q1/44 ,  C12Q1/48 ,  C12Q1/6806

CPC classification number: C12Q1/6869 ,  C12N15/1065 ,  C12Q1/44 ,  C12Q1/485 ,  C12Q1/6806 ,  C12Q2600/154 ,  G01N2333/91245 ,  G01N2333/922

Abstract: Methods are provided that implement tagmentation for single-molecule sequencing use 90-99% less input than current protocols: SMRT-Tag, which allows detection of genetic variation and CpG methylation, and SAMOSA-Tag, which uses exogenous adenine methylation to add a third channel for probing chromatin accessibility. SAMOSA-Tag of 30,000-50,000 nuclei resolved single-fiber chromatin structure, CTCF binding, and DNA methylation in patient-derived prostate cancer xenografts and uncovered metastasis-associated global epigenome disorganization.

公开(公告)号：US20240326048A1

公开(公告)日：2024-10-03

申请号：US18058122

申请日：2022-11-22

Applicant: MAMMOTH BIOSCIENCES, INC.

Inventor： Clare Louise FASCHING ,  Matthew VEROSLOFF ,  Jesus CHING ,  James Paul BROUGHTON ,  Maria-Nefeli TSALOGLOU ,  Phillip You Fai LEE ,  Timothy James PATNO ,  Janice Sha CHEN ,  Sarah Jane SHAPIRO ,  James Raymond CORDOVA ,  Brian J. EASTMAN ,  Donald Allan LOWMILLER ,  Lior KREINDLER ,  Daniel Thomas DRZAL ,  Devin SPRATT ,  Jacob LESINSKI ,  Joshua BAIK ,  Rachel Nouha DERANEY ,  Sonal JAIN ,  Sophia HUBBELL ,  Carley Gelenter HENDRIKS

IPC: B01L3/00 ,  B01L7/00 ,  C12N9/22 ,  C12N15/11 ,  C12Q1/48 ,  C12Q1/6806 ,  C12Q1/6823 ,  C12Q1/6834 ,  C12Q1/6844

CPC classification number: B01L3/502761 ,  B01L3/502715 ,  B01L7/52 ,  C12N9/22 ,  C12N15/111 ,  C12Q1/485 ,  C12Q1/6806 ,  C12Q1/6823 ,  C12Q1/6834 ,  C12Q1/6844 ,  B01L2200/16 ,  B01L2300/0681 ,  B01L2300/1877 ,  B01L2400/0487 ,  C12N2310/20

Abstract: The present disclosure provides various diagnostic devices. Devices can comprising a sample interface configured to receive a sample that may comprise at least one sequence of interest; a channel in fluid communication with the sample interface and a detection chamber, said channel comprising one or more movable mechanisms to separate the sample into a plurality of droplets, wherein said detection chamber is configured to receive and contact the plurality of droplets with at least one programmable nuclease probe disposed on a surface of said detection chamber, and wherein said at least one programmable nuclease probe may comprise a guide nucleic acid complexed with a programmable nuclease; and a plurality of sensors that determine a presence of said at least one sequence of interest by detecting a signal produced upon cleavage of a target nucleic acid region of said at least one sequence of interest by said at least one programmable nuclease probe.

公开(公告)号：US12098433B2

公开(公告)日：2024-09-24

申请号：US17265785

申请日：2019-08-05

Applicant: NORTHWESTERN UNIVERSITY

Inventor： Michael Christopher Jewett ,  Julius B. Lucks ,  Adam D. Silverman ,  Khalid K. Alam

IPC: C12Q1/6897 ,  C12Q1/48 ,  G01N33/18 ,  G01N33/52

CPC classification number: C12Q1/6897 ,  C12Q1/485 ,  G01N33/1826 ,  G01N33/521 ,  G01N33/184 ,  G01N2430/20

Abstract: Disclosed are methods, devices, kits, components, and compositions for detecting a target molecule in a test sample using a cell-free protein synthesis (CFPS) reaction. The methods, devices, kits, components, and compositions may be utilized for detecting target molecules which may include small molecules and/or metabolites of small molecules. The components and compositions used in the disclosed methods, devices, and kits may be dried or lyophilized and may be present or immobilized on a paper substrate, for example, as a paper test article.

公开(公告)号：US20240279723A1

公开(公告)日：2024-08-22

申请号：US18570190

申请日：2022-06-17

Applicant: NanoString Technologies, Inc.

Inventor： Lidan WU ,  Rustem KHAFIZOV ,  Evan Paul PERILLO ,  Dwayne DUNAWAY ,  Dae KIM ,  Joseph M. BEECHEM

IPC: C12Q1/6841 ,  C12Q1/48 ,  C12Q1/6806 ,  C12Q1/6874

CPC classification number: C12Q1/6841 ,  C12Q1/485 ,  C12Q1/6806 ,  C12Q1/6874 ,  G01N2333/9126

Abstract: The present disclosure is based in part on probes, compositions, methods, and kits for simultaneous, multiplexed spatial detection and quantification of protein and/or nucleic acid expression in a user-defined region of a tissue, user-defined cell, and/or user-defined subcellular structure within a cell.

公开(公告)号：US12054772B2

公开(公告)日：2024-08-06

申请号：US16979804

申请日：2019-03-13

Applicant: SARMAL, INC.

Inventor： Inanc Ortac

IPC: C12Q1/6869 ,  C12Q1/48 ,  C12Q1/66

CPC classification number: C12Q1/6869 ,  C12Q1/485 ,  C12Q1/66

Abstract: Provided herein are methods and systems for sequencing a single nucleic acid molecule utilizing a polymerase enzyme, a template nucleic acid, and a polymerase reagent solution, including components for a luminescence reaction.

公开(公告)号：US12006555B2

公开(公告)日：2024-06-11

申请号：US16854654

申请日：2020-04-21

Applicant: The General Hospital Corporation ,  Dana-Farber Cancer Institute, Inc.

Inventor： Daphne Winifred Bell ,  Daniel A. Haber ,  Pasi Antero Janne ,  Bruce E. Johnson ,  Thomas J. Lynch ,  Matthew Meyerson ,  Juan Guillermo Paez ,  William R. Sellers ,  Jeffrey E. Settleman ,  Raffaella Sordella

IPC: C12Q1/68 ,  C12Q1/48 ,  C12Q1/6886 ,  G01N33/574 ,  G01N33/74

CPC classification number: C12Q1/6886 ,  C12Q1/485 ,  G01N33/574 ,  G01N33/74 ,  C12Q2600/106 ,  C12Q2600/118 ,  C12Q2600/136 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16 ,  G01N2333/485 ,  G01N2800/52

Abstract: Disclosed herein are methods and reagents for determining the responsiveness of cancer to an epidermal growth factor receptor (EGFR) targeting treatment. The detection of these mutations will allow for the administration of gefitinib, erlotinib and other tyrosine kinase inhibitors to those patients most likely to respond to the drug.

公开(公告)号：US11963957B2

公开(公告)日：2024-04-23

申请号：US17114376

申请日：2020-12-07

Applicant: Buck Institute for Research on Aging ,  Unity Biotechnology, Inc. ,  Mayo Foundation for Medical Education and Research

Inventor： Remi-Martin Laberge ,  Judith Campisi ,  Albert Davalos ,  Marco Demaria ,  Nathaniel David ,  Jan M. A. van Deursen ,  Darren J. Baker ,  Bennett G. Childs

IPC: A61P9/10 ,  A61K9/00 ,  A61K31/404 ,  A61K31/4178 ,  A61K31/428 ,  A61K31/4375 ,  A61K31/495 ,  A61K31/496 ,  A61K31/5377 ,  A61K31/728 ,  A61K45/06 ,  A61K47/36 ,  A61K47/68 ,  A61P11/00 ,  A61P25/28 ,  A61P27/02 ,  C12N5/00 ,  C12N15/113 ,  C12Q1/48

CPC classification number: A61K31/496 ,  A61K9/0048 ,  A61K9/0073 ,  A61K31/404 ,  A61K31/4178 ,  A61K31/428 ,  A61K31/4375 ,  A61K31/495 ,  A61K31/5377 ,  A61K31/728 ,  A61K45/06 ,  A61K47/36 ,  A61K47/6807 ,  A61P9/10 ,  A61P11/00 ,  A61P25/28 ,  A61P27/02 ,  C12N5/0081 ,  C12N15/113 ,  C12N2320/30 ,  C12N2501/999 ,  C12N2503/02 ,  C12Q1/485

Abstract: Methods are provided herein for selectively killing senescent cells and for treating senescence-associated diseases and disorders by administering a senolytic agent. Senescence-associated diseases and disorders treatable by the methods using the senolytic agents described herein include cardiovascular diseases and disorders associated with or caused by arteriosclerosis, such as atherosclerosis; idiopathic pulmonary fibrosis; chronic obstructive pulmonary disease; osteoarthritis; senescence-associated ophthalmic diseases and disorders; and senescence-associated dermatological diseases and disorders.

公开(公告)号：US11952627B2

公开(公告)日：2024-04-09

申请号：US18448618

申请日：2023-08-11

Applicant: 10x Genomics, Inc.

Inventor： Marlon Stoeckius

IPC: C12Q1/68 ,  C12Q1/48 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q1/6876

CPC classification number: C12Q1/6874 ,  C12Q1/485 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q1/6876 ,  C12Y207/07007 ,  C12Y301/26004 ,  C12Y605/01001 ,  G01N2333/9015 ,  G01N2333/9126 ,  G01N2333/922

Abstract: Provided herein are methods of identifying a location of an RNA in a sample that include: (a) contacting the sample with an array comprising capture probes, where a capture probe comprises a capture domain and a spatial barcode; (b) releasing the RNA from the sample; (c) extending a 3′ end of the capture probe using the capture domain-bound RNA as a template; (d) generating nick(s) in the extended capture probe-hybridized RNA and performing random-primed DNA synthesis; (e) performing end repair on the second strand DNA molecule; (f) adding a single adenosine nucleotide to the 3′ end of the extended capture probe; (g) ligating a double-stranded sequencing adaptor to the double-stranded DNA product; and (h) determining all or a part of the sequence of the RNA, and the sequence of the spatial barcode, or complements thereof, and using the determined sequences to identify the location of the RNA in the sample.

公开(公告)号：US20240110188A1

公开(公告)日：2024-04-04

申请号：US18479246

申请日：2023-10-02

Applicant: Conopco, Inc., d/b/a UNILEVER

Inventor： Luba Milena PARDO CORTES ,  Rebecca Susan GINGER ,  David Andrew GUNN ,  Tamarius Edmond Christoffel NIJSTEN ,  Martijn Gerard Hendrik SANDERS ,  Adrian Michael SMITH

IPC: C12N15/113 ,  A61K8/04 ,  A61Q5/00 ,  A61Q5/02 ,  A61Q5/12 ,  C12Q1/48 ,  G01N33/68

CPC classification number: C12N15/1137 ,  A61K8/042 ,  A61Q5/006 ,  A61Q5/02 ,  A61Q5/12 ,  C12Q1/485 ,  C12Y207/11001 ,  G01N33/6881 ,  C12N2310/14 ,  G01N2500/04

Abstract: Disclosed is a substance which down-regulates the activity of a MAST gene, or the activity of a transcription or translation product of a MAST gene, for use in the prevent and/or treatment of an inflammatory skin condition in a mammalian subject.

公开(公告)号：US11866764B2

公开(公告)日：2024-01-09

申请号：US16989835

申请日：2020-08-10

Applicant: THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA

Inventor： Gideon Dreyfuss ,  Mumtaz Kasim

IPC: A61K31/496 ,  A61P25/28 ,  C12Q1/48 ,  C07D241/04

CPC classification number: C12Q1/485 ,  A61P25/28 ,  A61K31/496 ,  C07D241/04 ,  G01N2500/10

Abstract: This invention provides compounds that inhibit p38 mitogen-activated protein kinase. Moreover, the invention provides methods for testing a candidate compound for a p38 mitogen-activated protein kinase modifying activity by calculating the level of relocalization of an SMN complex component from the cytoplasm to the nucleus of a cell. Additionally, the invention provides a kit and a system for calculating the same.