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1.发明公开公开(公告)号：US20240352481A1
公开(公告)日：2024-10-24
申请号：US18292590
申请日：2022-07-29
申请人： VOR BIOPHARMA INC.
IPC分类号： C12N15/86 , C12N5/0783 , C12Q1/6897 , G01N33/50
CPC分类号： C12N15/86 , C12N5/0636 , C12Q1/6897 , G01N33/505 , C12N2510/00 , C12N2740/16043 , C12N2830/001 , C12N2830/15 , C12N2840/20
摘要： Provided herein are IL-2 reporter systems comprising nucleic acid constructs comprising a nucleotide sequence encoding a reporter molecule operably linked to a minimal nuclear factor of activated T cells (NFAT)-responsive promoter. Also provided herein are vectors, cells, and cell lines comprising such nucleic acids. Also provided herein are methods of making and using such cells, for example to measure the ability of a chimeric antigen receptor (CAR) to induce nuclear factor of activated T cells (NFAT)-signaling in a cell.
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公开(公告)号：US12117402B2
公开(公告)日：2024-10-15
申请号：US17386917
申请日：2021-07-28
CPC分类号： G01N21/763 , C12N1/20 , C12Q1/02 , C12Q1/6897 , G01N1/28 , G01N33/24 , G01N33/5097 , C12R2001/39
摘要： Disclosed are isothiocyanate (ITC)-detecting biosensors that utilize recombinant host cells containing an ITC responsive genetic element such as a saxA promoter, operably linked with a reporter element, such as a luxCDABE operon or ilux operon. Such biosensors can detect the presence of diverse ITCs in samples such as plant extracts, biofumigated soils and seed meal amended soils.
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公开(公告)号：US20240309402A1
公开(公告)日：2024-09-19
申请号：US18418248
申请日：2024-01-20
发明人： Christopher Anthony Vakulskas , Nicole Mary Bode , Michael Allen Collingwood , Garrett Richard Rettig , Mark Aaron Behlke
CPC分类号： C12N15/87 , C12N9/22 , C12N15/102 , C12N15/88 , C12N15/90 , C12Q1/6897 , C12N2310/20 , C12N2810/50 , C12P19/34
摘要： This invention pertains to isolated mutant Cas9 nucleic acids and proteins for use in CRISPR/Cas endonuclease systems and their methods of use. In particular, the invention pertains to an isolated mutant Cas9 protein, wherein the isolated mutant Cas9 protein is active in a CRISPR/Cas endonuclease system, wherein the CRISPR/Cas endonuclease system displays a lower ratio of reduced off-target editing activity to on-target editing activity for at least one target site relative to the corresponding ratio of reduced off-site editing activity to on-target editing activity of a wild-type CRISPR/Cas endonuclease system having a WT-Cas9 protein of SEQ ID NO:5.
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公开(公告)号：US20240287626A1
公开(公告)日：2024-08-29
申请号：US18499563
申请日：2023-11-01
申请人： SyntheX, Inc.
发明人： Maria Soloveychik , Charly Chahwan
IPC分类号： C12Q1/6897 , C12N15/10 , C12N15/80 , C12N15/81
CPC分类号： C12Q1/6897 , C12N15/1055 , C12N15/1093 , C12N15/80 , C12N15/81 , C12N15/815 , C12Q2521/50 , C12Q2600/158
摘要： The present disclosure provides methods to identify peptides and small molecule moieties that are able to modulate protein-protein interactions (PPIs). Some moieties can disrupt specific PPIs within a complex, or disrupt variant-specific PPIs. Some moieties can alternatively bridge between two proteins in a protein-specific or a variant-specific manner. The methods described enable generation of compounds able to modulate PPI networks within cells with implications for drug development for pathological conditions.
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公开(公告)号：US20240271187A1
公开(公告)日：2024-08-15
申请号：US18022098
申请日：2021-08-18
发明人： William Jeremy Blake , Paul David Carlson , Elizabeth Anne Phillips , Michael Menglong Liu , Carl Wayne Brown, III , Mary Katherine Wilson , Brendan John Manning
IPC分类号： C12Q1/6825 , C12Q1/6897
CPC分类号： C12Q1/6825 , C12Q1/6897
摘要： The present disclosure provides compositions and methods that permit detection of nucleic acids in samples (e.g., biological and/or environmental samples). The present disclosure describes “split reporter” sensor system technologies.
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公开(公告)号：US20240229100A1
公开(公告)日：2024-07-11
申请号：US18411772
申请日：2024-01-12
IPC分类号： C12Q1/10 , A61K35/76 , C12N7/00 , C12N15/65 , C12N15/74 , C12N15/86 , C12Q1/02 , C12Q1/66 , C12Q1/6897 , C12Q1/70 , G01N33/50
CPC分类号： C12Q1/10 , A61K35/76 , C12N7/00 , C12N15/65 , C12N15/74 , C12N15/86 , C12Q1/025 , C12Q1/66 , C12Q1/6897 , C12Q1/70 , G01N33/50 , C12N2795/00021 , C12N2795/00031 , C12N2795/00051 , C12N2795/10131 , C12N2795/10143 , C12Q2304/60 , G01N2800/52
摘要： Disclosed herein are methods and systems for rapid detection of microorganisms in a sample. A genetically modified bacteriophage is also disclosed which comprises an indicator gene in the late gene region. The specificity of the bacteriophage, such as Salmonella-specific bacteriophage, allows detection of a specific microorganism, such as Salmonella spp. and an indicator signal may be amplified to optimize assay sensitivity.
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公开(公告)号：US11981954B2
公开(公告)日：2024-05-14
申请号：US17812380
申请日：2022-07-13
发明人： Kyle C. Cady , Brett Hanson , Paulino Abdon , Ryan Chan , Patrick Lin , Rochak Mehta , Troy Rabang
IPC分类号： C12P19/34 , C12Q1/18 , C12Q1/689 , C12Q1/6895 , C12Q1/6897 , C12Q1/6851 , C12Q1/686
CPC分类号： C12Q1/18 , C12Q1/689 , C12Q1/6895 , C12Q1/6897 , C12Q1/6851 , C12Q1/686 , C12Q2561/101 , C12Q2561/113 , C12Q2565/1015 , C12Q2600/136
摘要： The present invention relates to compositions and methods for the use of polymerase chain reaction (PCR) as a reporter assay for rapid and simultaneous bacterial identification and phenotype testing for antimicrobial susceptibility (AST). The current invention uses a strategy that has shown the ability for multiplexing and for handling polymicrobial samples for antimicrobial susceptibility testing.
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公开(公告)号：US20240124944A1
公开(公告)日：2024-04-18
申请号：US18525193
申请日：2023-11-30
IPC分类号： C12Q1/6897 , C12Q1/6883
CPC分类号： C12Q1/6897 , C12Q1/6883 , C12Q2600/136 , C12Q2600/158
摘要： The present invention relates to systems and methods for screening compounds and/or mutant ribosomal proteins in a eukaryotic cell that increase or decrease the translation of a target gene and thereby ameliorate or revert a defective and/or undesired translation of a target gene. Disclosed are compounds and proteins as identified with the methods and systems of the invention, pharmaceutical and cosmetic compositions thereof, their uses for the preparation of a medicament, methods of treatment of a disease or condition or cosmetic condition related to the defective translation of a gene, for example genetic diseases such as Epidermolysis bullosa, as well as diagnostic measures practical for the clinical evaluation of such diseases or conditions. Also, kits are provided which comprise the identified compounds and/or proteins in addition to suitable means for performing the methods of the invention.
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公开(公告)号：US11905521B2
公开(公告)日：2024-02-20
申请号：US15354329
申请日：2016-11-17
发明人： Bo Feng , Xiangjun He
IPC分类号： C12N15/00 , C12N15/85 , C12Q1/6897 , C12N9/22 , C12N15/10
CPC分类号： C12N15/85 , C12N9/22 , C12N15/102 , C12N15/1086 , C12Q1/6897
摘要： The present invention provides novel methods and systems, including cell lines, recombinant polynucleotide constructs, compositions, and kits, for targeted yet universal genomic manipulation.
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公开(公告)号：US11875298B2
公开(公告)日：2024-01-16
申请号：US16518544
申请日：2019-07-22
发明人： Solvej Siedler , Georg Schendzielorz , Stephan Binder , Lothar Eggeling , Stephanie Bringer-Meyer , Michael Bott
IPC分类号： C12Q1/6897 , G06Q10/0639 , G06N5/045 , A61K31/07 , C12N15/70 , G06N3/006 , G06Q10/04 , C12Q1/26 , C12Q1/68
CPC分类号： G06Q10/06395 , A61K31/07 , C12N15/70 , C12Q1/6897 , C12Y101/00 , G06N3/006 , G06N5/045 , G06Q10/04 , C12Q1/26 , C12Q1/68
摘要： An NADP(H) nanosensor has i) a nucleic acid sequence to which a regulator is capable of binding, wherein the oxidation state of the regulator depends on the NADP(H) availability; ii) a promoter sequence following the nucleic acid sequence i), to which an RNA polymerase is capable of binding, wherein the affinity of the RNA polymerase for the promoter sequence is influenced by the oxidation state of the regulator; iii) a nucleic acid sequence which is under the control of the promoter sequence ii) and which codes for an autofluorescent protein. The present invention also relates to a cell, a method for isolating genes which code for NADP(H)-dependent enzymes, and the use of an NADP(H) nanosensor.
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