公开(公告)号：US12123012B2

公开(公告)日：2024-10-22

申请号：US17104552

申请日：2020-11-25

Applicant: LIGANDAL, INC.

Inventor： Andre Ronald Watson ,  Christian Foster

IPC: A61K48/00 ,  A61K9/00 ,  A61K9/51 ,  A61K31/40 ,  A61K31/7105 ,  A61K47/34 ,  A61K47/42 ,  A61K47/54 ,  A61K47/64 ,  A61K47/68 ,  A61P3/10 ,  A61P35/00 ,  A61P37/00 ,  B82Y5/00 ,  C12N15/10 ,  C12N15/11 ,  C12N15/85 ,  C12N15/87 ,  C12N9/22

CPC classification number: C12N15/85 ,  A61K9/0019 ,  A61K9/0085 ,  A61K9/5146 ,  A61K31/40 ,  A61K31/7105 ,  A61K47/34 ,  A61K47/42 ,  A61K47/549 ,  A61K47/64 ,  A61K47/645 ,  A61K47/6455 ,  A61K47/6807 ,  A61K48/0016 ,  A61K48/0041 ,  A61K48/0075 ,  A61P3/10 ,  A61P35/00 ,  A61P37/00 ,  B82Y5/00 ,  C12N15/102 ,  C12N15/111 ,  C12N15/87 ,  C12N9/22 ,  C12N2310/14 ,  C12N2310/20 ,  C12N2310/3517 ,  C12N2320/32

Abstract: Provided are methods and compositions for delivering a nucleic acid, protein, and/or ribonucleoprotein payload to a cell. Also provided are delivery molecules that include a peptide targeting ligand conjugated to a protein or nucleic acid payload (e.g., an siRNA molecule), or conjugated to a charged polymer polypeptide domain (e.g., poly-arginine such as 9R or a poly-histidine such as 6H, and the like). The targeting ligand provides for (i) targeted binding to a cell surface protein, and (ii) engagement of a long endosomal recycling pathway. As such, when the targeting ligand engages the intended cell surface protein, the delivery molecule enters the cell (e.g., via endocytosis) but is preferentially directed away from the lysosomal degradation pathway.

公开(公告)号：US20240336937A1

公开(公告)日：2024-10-10

申请号：US18285057

申请日：2022-03-28

Applicant: CTCELLS, INC.

Inventor： Jung Min LEE

IPC: C12N15/87 ,  C12M3/06

CPC classification number: C12N15/87 ,  C12M23/16 ,  C12N2740/15043

Abstract: Provided are a cell transduction method and a microfluidic chip for cell transduction. According to the present disclosure, since traits are introduced using a microfluidic-based method, it is possible to avoid cell stress induced by strong centrifugal force and to achieve a high multiplicity of infection (MOI) even with a small volume, thereby being efficiently used for cell transformation.

公开(公告)号：US20240327799A1

公开(公告)日：2024-10-03

申请号：US18745761

申请日：2024-06-17

Applicant: ElevateBio Technologies, Inc.

Inventor： Melissa CARPENTER ,  Santosh NARAYAN ,  Austin THIEL ,  Yin Miranda YANG

IPC: C12N5/074 ,  A61K35/545 ,  C12N9/22 ,  C12N15/11 ,  C12N15/113 ,  C12N15/87

CPC classification number: C12N5/0696 ,  A61K35/545 ,  C12N9/22 ,  C12N15/111 ,  C12N15/113 ,  C12N15/87 ,  C12N2310/141 ,  C12N2310/20 ,  C12N2501/01 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2506/11 ,  C12N2510/00

Abstract: The present disclosure provides methods of producing iPSCs comprising contacting a cell (e.g., CD34+ cell or other blood cell) in suspension with one or more circular RNAs encoding one or more reprogramming factors and maintaining the cell under conditions under which a reprogrammed iPSC is obtained. In some embodiments, the circular RNA encodes a reprogramming factor (selected from Oct3/4, Klf4, Sox2, Nanog, Lin28, c-Myc, or L-Myc, or a fragment or variant thereof.

公开(公告)号：US20240327478A1

公开(公告)日：2024-10-03

申请号：US18490611

申请日：2023-10-19

Applicant: President and Fellows of Harvard College ,  The Board of Trustees of the Leland Stanford Junior University

Inventor： Quan Lu ,  Qiyu Wang ,  Stanley N. Cohen

IPC: C07K14/47 ,  C12N9/22 ,  C12N15/10 ,  C12N15/87

CPC classification number: C07K14/4702 ,  A61K47/6455 ,  C07K7/06 ,  C07K14/4718 ,  C12N9/16 ,  C12N9/22 ,  C12N9/93 ,  C12N15/102 ,  C12N15/87 ,  A61K9/1272 ,  C07K2319/00 ,  C07K2319/01 ,  C07K2319/03 ,  C07K2319/09 ,  C07K2319/70 ,  C07K2319/85 ,  C12N2310/20 ,  C12N2810/85

Abstract: Methods, systems, compositions and strategies for the delivery of WW domain-containing fusion proteins into cells in vivo, ex vivo, or in vitro via ARMMs are provided. Methods, systems, compositions and strategies for the delivery of Cas9 proteins and/or Cas9 variants into cells in vivo, ex vivo, or in vitro via fusion to ARMM associated proteins (e.g., ARRDC1 or TSG101) are also provided.

公开(公告)号：US20240325659A1

公开(公告)日：2024-10-03

申请号：US18575953

申请日：2022-07-06

Applicant: Daicel Corporation

Inventor： Naoki SAKAGUCHI ,  Kunihiko YAMASHITA ,  Kazuhiro TERAI

IPC: A61M5/48 ,  A61K9/00 ,  A61K48/00 ,  A61M5/20 ,  A61M5/30 ,  C12N15/87

CPC classification number: A61M5/482 ,  A61K9/0019 ,  A61K48/0016 ,  A61M5/2046 ,  A61M5/30 ,  C12N15/87

Abstract: Provided herein is a method of administering a naked nucleic acid molecule to a subject. The method may include injecting the naked nucleic acid molecule to the subject, wherein the injecting exhibits a bi-phasic injection profile comprising a first phase and a second phase, the second phase being after the first phase, and the bi-phasic injection profile has (i) at least two peaks within 15 msec from the injecting, (ii) the first peak of at least 2 MPa, or (iii) the highest peak of the second phase of the bi-phasic injection within 30 msec from the injecting.

公开(公告)号：US20240294939A1

公开(公告)日：2024-09-05

申请号：US18663233

申请日：2024-05-14

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Prashant G. Mali ,  Luhan Yang

IPC: C12N15/85 ,  C12N9/22 ,  C12N15/01 ,  C12N15/10 ,  C12N15/63 ,  C12N15/81 ,  C12N15/82 ,  C12N15/87 ,  C12N15/90

CPC classification number: C12N15/85 ,  C12N9/22 ,  C12N15/01 ,  C12N15/10 ,  C12N15/102 ,  C12N15/1024 ,  C12N15/63 ,  C12N15/81 ,  C12N15/8201 ,  C12N15/87 ,  C12N15/90 ,  C12N15/907 ,  C12N2310/20 ,  C12N2800/80 ,  C12N2810/55 ,  C12Y301/00

Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.

公开(公告)号：US12077774B2

公开(公告)日：2024-09-03

申请号：US17048239

申请日：2019-04-17

Applicant: UCL Business Ltd.

Inventor： Hans Stauss ,  Jenny L. McGovern

IPC: C12N15/87 ,  A61K35/12 ,  A61K35/17 ,  A61K38/00 ,  C07K14/725 ,  C07K14/74 ,  C12N5/0783

CPC classification number: C12N15/87 ,  A61K35/17 ,  C07K14/7051 ,  C07K14/70539 ,  C12N5/0637 ,  A61K2035/122 ,  A61K38/00 ,  C12N2510/00

Abstract: The present invention relates to a method for enhancing the ability of regulatory T cells (Tregs) to suppress immune responses comprising increasing FOXP3 expression in a population of Tregs.

公开(公告)号：US20240285774A1

公开(公告)日：2024-08-29

申请号：US18503905

申请日：2023-11-07

Applicant: Yale University

Inventor： Elias QUIJANO ,  Peter GLAZER

IPC: A61K47/54 ,  A61K39/00 ,  A61K47/68 ,  A61K48/00 ,  A61P35/00 ,  C07K14/47 ,  C07K14/52 ,  C07K16/44 ,  C12N15/11 ,  C12N15/113 ,  C12N15/87

CPC classification number: A61K47/549 ,  A61K47/6807 ,  A61K47/6851 ,  A61K48/0025 ,  A61K48/005 ,  A61P35/00 ,  C07K14/4747 ,  C07K14/52 ,  C07K16/44 ,  C12N15/111 ,  C12N15/113 ,  C12N15/1135 ,  C12N15/87 ,  A61K2039/505 ,  A61K48/00 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/565 ,  C07K2317/622 ,  C07K2317/77 ,  C07K2319/33 ,  C12N2310/11 ,  C12N2310/14 ,  C12N2310/3181 ,  C12N2310/3513 ,  C12N2320/32

Abstract: Compositions and methods of use thereof for delivering nucleic acid cargo into cells are provided. The compositions typically include (a) a 3E10 monoclonal antibody or an antigen binding, cell-penetrating fragment thereof; a monovalent, divalent, or multivalent single chain variable fragment (scFv); or a diabody; or humanized form or variant thereof, and (b) a nucleic acid cargo including, for example, a nucleic acid encoding a polypeptide, a functional nucleic acid, a nucleic acid encoding a functional nucleic acid, or a combination thereof. Elements (a) and (b) are typically non-covalently linked to form a complex.

公开(公告)号：US20240279677A1

公开(公告)日：2024-08-22

申请号：US18619354

申请日：2024-03-28

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Prashant G. Mali ,  Luhan Yang

IPC: C12N15/85 ,  C12N9/22 ,  C12N15/01 ,  C12N15/10 ,  C12N15/63 ,  C12N15/81 ,  C12N15/82 ,  C12N15/87 ,  C12N15/90

CPC classification number: C12N15/85 ,  C12N9/22 ,  C12N15/01 ,  C12N15/10 ,  C12N15/102 ,  C12N15/1024 ,  C12N15/63 ,  C12N15/81 ,  C12N15/8201 ,  C12N15/87 ,  C12N15/90 ,  C12N15/907 ,  C12N2310/20 ,  C12N2800/80 ,  C12N2810/55 ,  C12Y301/00

Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.

公开(公告)号：US12064484B2

公开(公告)日：2024-08-20

申请号：US14901467

申请日：2014-06-27

Applicant: ETHRIS GMBH

Inventor： Christian Dohmen ,  Christian Plank ,  Carsten Rudolph ,  Christian Koch

IPC: A61K47/00 ,  A61K9/19 ,  A61K31/7088 ,  A61K31/713 ,  A61K47/26 ,  A61K47/58 ,  A61K47/59 ,  A61K48/00 ,  C08F8/32 ,  C08G73/02 ,  C12N15/87

CPC classification number: A61K48/0041 ,  A61K9/19 ,  A61K31/713 ,  A61K47/26 ,  A61K47/58 ,  A61K47/59 ,  C08F8/32 ,  C08G73/0206 ,  C08G73/0213 ,  C12N15/87

Abstract: The present invention relates to oligomers, polymers and lipidoids comprising characteristic oligo(alkylene amine) moieties which are useful as vehicles for transfecting a cell with RNA. The present invention furthermore relates to a composition comprising at least a nucleic acid and an oligomer or polymer or a lipidoid comprising such oligo(alkylene amine) moieties and to a method of transfecting a cell using said composition. Furthermore, the present invention relates to pharmaceutical compositions and uses.