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    Regulation of angiogenesis with zinc finger proteins
    7.
    发明授权
    Regulation of angiogenesis with zinc finger proteins 失效
    用锌指蛋白调节血管生成

    公开(公告)号：US08071564B2

    公开(公告)日：2011-12-06

    申请号：US12553826

    申请日：2009-09-03

    申请人： Edward Rebar ,  Andrew Jamieson ,  Qiang Liu ,  Pei-Qi Liu ,  Alan Wolffe ,  Stephen P. Eisenberg ,  Eric Jarvis

    发明人： Edward Rebar ,  Andrew Jamieson ,  Qiang Liu ,  Pei-Qi Liu ,  Alan Wolffe ,  Stephen P. Eisenberg ,  Eric Jarvis

    IPC分类号： A61K48/00 ,  C07H21/02

    CPC分类号： A61K48/005 ,  A61K38/00 ,  A61K48/00 ,  C07K14/47 ,  C07K14/4702 ,  C07K14/52 ,  C07K2319/00 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2740/13043 ,  C12N2799/021 ,  C12N2810/85 ,  C12N2830/008

    摘要： Provided herein are a variety of methods and compositions for regulating angiogenesis, such methods and compositions being useful in a variety of applications where modulation of vascular formation is useful, including, but not limited to, treatments for ischemia and wound healing. Certain of the methods and compositions accomplish this by using various zinc finger proteins that bind to particular target sites in one or more VEGF genes. Nucleic acids encoding the zinc finger proteins are also disclosed. Methods for modulating the expression of one or more VEGF genes with the zinc finger proteins and nucleic acids are also disclosed. Such methods can also be utilized in a variety of therapeutic applications that involve the regulation of endothelial cell growth. Pharmaceutical compositions including the zinc finger proteins or nucleic acids encoding them are also provided.

    摘要翻译： 本文提供了用于调节血管生成的各种方法和组合物，所述方法和组合物可用于血管形成调节是有用的多种应用，包括但不限于缺血和伤口愈合的治疗。 某些方法和组合物通过使用结合一个或多个VEGF基因中的特定靶位点的各种锌指蛋白来实现。 还公开了编码锌指蛋白的核酸。 还公开了用锌指蛋白和核酸调节一种或多种VEGF基因的表达的方法。 这些方法也可用于涉及内皮细胞生长调节的各种治疗应用中。 还提供了包括锌指蛋白或编码它们的核酸的药物组合物。

    Method and kit for expressing protein under regulation of the expression from repeated sequence formed by gene amplification, and transformant
    9.
    发明申请
    Method and kit for expressing protein under regulation of the expression from repeated sequence formed by gene amplification, and transformant 有权
    用于通过基因扩增形成的重复序列表达调控表达蛋白质的方法和试剂盒，以及转化体

    公开(公告)号：US20080032341A1

    公开(公告)日：2008-02-07

    申请号：US11889512

    申请日：2007-08-14

    申请人： Noriaki Shimizu

    发明人： Noriaki Shimizu

    IPC分类号： C12P21/00 ,  C12N5/10

    CPC分类号： C12N15/67 ,  C12N15/85 ,  C12N2810/85 ,  C12N2820/85 ,  C12P21/02

    摘要： A method is disclosed for releasing the transcriptional regulation caused by a repeated sequence in a gene, a kit therefor and so on to thereby establish a system capable of producing a protein in a large amount. At least one embodiment of the method can be achieved by any one or more of the following methods: (a) in the amplification of a gene encoding a target protein, co-amplifying a polynucleotide of 10 kbp or more such as a α-phage DNA or an insulator sequence; (b) selecting by culturing cells having undergone gene amplification in media containing a drug with a gradual increase in concentration; (c) elevating the promoter activity of inducing the expression of a gene encoding a target protein; (d) excising an amplified gene region from a chromosome with the use of Cre-LoxP System; (e) treating cells having undergone gene amplification with 5-aza-2′-deoxycytidine to thereby lower the methylation degree of DNA; and (f) selecting the mammalian cells having undergone gene amplification on double minute chromosomes.

    摘要翻译： 公开了一种用于释放由基因中的重复序列引起的转录调控的方法，其用于其的试剂盒等，从而建立能够大量产生蛋白质的系统。 该方法的至少一个实施方案可以通过以下任何一种或多种方法来实现：（a）扩增编码靶蛋白的基因，共扩增10kbp或更多的多核苷酸，例如α-噬菌体 DNA或绝缘子序列; （b）通过培养在具有逐渐增加浓度的药物的培养基中培养经历基因扩增的细胞进行选择; （c）提高诱导编码靶蛋白的基因表达的启动子活性; （d）使用Cre-LoxP系统从染色体切除扩增的基因区域; （e）处理已经用5-氮杂-2'-脱氧胞苷进行基因扩增的细胞，从而降低DNA的甲基化程度; 和（f）选择在双分子染色体上经历基因扩增的哺乳动物细胞。

    Viral vectors with improved properties
    10.
    发明申请
    Viral vectors with improved properties 审中-公开
    具有改善性质的病毒载体

    公开(公告)号：US20060286545A1

    公开(公告)日：2006-12-21

    申请号：US10558394

    申请日：2004-05-24

    申请人： Thomas Weber ,  Laure Gigout

    发明人： Thomas Weber ,  Laure Gigout

    IPC分类号： C40B30/06 ,  C40B40/08 ,  C12N15/86

    CPC分类号： C12N15/86 ,  A61K48/0091 ,  C12N7/00 ,  C12N2750/14123 ,  C12N2750/14143 ,  C12N2750/14145 ,  C12N2810/40 ,  C12N2810/405 ,  C12N2810/60 ,  C12N2810/6072 ,  C12N2810/80 ,  C12N2810/85 ,  C12N2810/851 ,  C12N2810/854 ,  C12N2810/859 ,  C12N2830/42 ,  C12N2840/44

    摘要： Methods to improve the tropism or other features of a virus is disclosed. Such methods can be used to prepare, e.g., DNA or plasmid libraries of variants of a gene encoding a viral capsid or envelope restriction site, libraries of viral clones with such variant genes with a randon-dy inserted restriction site or polypeptide sequence targeting a receptor expressed by a specific type of mammalian cells. Described are also methods to prepare mosaic viruses, i.e., viral particles wherein copies of one or more capsid or envelope proteins originate from different sources. These methods can be used to prepare mosaic viruses of a specific mixture of wild-type and mutant proteins, or of different types of mutant proteins.

    摘要翻译： 公开了改善病毒的向性或其他特征的方法。 这样的方法可以用于制备例如编码病毒衣壳或包膜限制性位点的基因的变体的DNA或质粒文库，具有这样的变体基因的病毒克隆的文库与插入限制性位点或靶向受体的多肽序列 由特定类型的哺乳动物细胞表达。 还描述了制备花叶病毒的方法，即病毒颗粒，其中一种或多种衣壳或包膜蛋白的拷贝来源于不同的来源。 这些方法可用于制备野生型和突变蛋白或不同类型的突变蛋白的特异性混合物的花叶病毒。