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    Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites
    7.
    发明授权
    Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites 有权
    选择锌指蛋白靶向位点和设计锌指蛋白结合预选位点的方法

    公开(公告)号：US06453242B1

    公开(公告)日：2002-09-17

    申请号：US09229007

    申请日：1999-01-12

    申请人： Stephen P. Eisenberg ,  Casey C. Case ,  George N. Cox, III ,  Andrew Jamieson ,  Edward J. Rebar

    发明人： Stephen P. Eisenberg ,  Casey C. Case ,  George N. Cox, III ,  Andrew Jamieson ,  Edward J. Rebar

    IPC分类号： G06F1900

    CPC分类号： C12N15/1089 ,  C07K14/47 ,  C07K14/4702 ,  C12Q1/6811 ,  G01N33/5308 ,  G01N33/6875 ,  G06F19/18

    摘要： The invention provides criteria and methods for selecting optimum subsequence(s) from a target gene for targeting by a zinc finger protein. Some of the methods of target site selection seek to identify one or more target segments having a DNA motif containing one or more so-called D-able subsites having the sequence 5′NNGK3′. Other methods of the invention are directed to selection of target segments within target genes using a correspondence regime between different triplets of three bases and the three possible positions of a triplet within a nine-base site. In another aspect, the invention provides methods of designing zinc finger proteins that bind to a preselected target site. These methods can be used following the preselection of target sites according to the procedures and criteria described above. The methods of design use a database containing information about previously characterized zinc finger proteins.

    摘要翻译： 本发明提供了从靶基因中选择锌指蛋白靶向的最佳亚序列的标准和方法。 靶位点选择的一些方法寻求鉴定具有含有一个或多个具有序列5'NNGK3'的一个或多个所谓的D能子集的DNA基序的一个或多个靶区段。 本发明的其它方法涉及使用三个碱基的不同三联体之间的对应方式和九个碱基位点内三联体的三个可能位置来选择靶基因内的靶区段。 另一方面，本发明提供了设计结合预选靶位点的锌指蛋白的方法。 这些方法可以根据上述程序和标准在预选目标位点之后使用。 设计方法使用包含以前特征的锌指蛋白的信息的数据库。

    High Pressure Treatment of Aggregated Interferons
    10.
    发明申请
    High Pressure Treatment of Aggregated Interferons 审中-公开
    聚合干扰素的高压处理

    公开(公告)号：US20130058895A1

    公开(公告)日：2013-03-07

    申请号：US13560979

    申请日：2012-07-27

    申请人： Jeffrey L. Cleland ,  Stephen P. Eisenberg ,  Mary S. Rosendahl ,  Matthew B. Seefeldt

    发明人： Jeffrey L. Cleland ,  Stephen P. Eisenberg ,  Mary S. Rosendahl ,  Matthew B. Seefeldt

    IPC分类号： C07K14/555 ,  A61K38/21 ,  C07K14/57 ,  C07K14/56 ,  C07K14/565

    CPC分类号： C07K14/555 ,  A61K38/212 ,  A61K38/215 ,  A61K38/217 ,  C07K14/565

    摘要： Methods are provided using high pressure to treat aggregated interferons, to reduce the aggregate content of interferon material. In particular, recombinant human interferon-β may be so treated. Multiple strategies may be used in combination to make nonglycosylated IFN-β more amenable to high pressure treatment. When coupled with purification techniques, these strategies singly or in combination provide a low aggregate or substantially aggregate free, biologically active solution. In certain aspects, pharmaceutical compositions containing nonglycosylated interferon having less than about 5 weight percent of protein aggregation are provided.

    摘要翻译： 提供使用高压处理聚集的干扰素的方法，以减少干扰素材料的总含量。 特别是重组人干扰素 可能会如此对待 可以组合使用多种策略以制备非糖基化IFN-和bgr; 更适合高压处理。 当与纯化技术结合时，这些策略单独或组合提供了低聚集体或基本上聚集的游离的生物活性溶液。 在某些方面，提供了含有小于约5重量％蛋白质聚集的非糖基化干扰素的药物组合物。