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    Position dependent recognition of GNN nucleotide triplets by zinc fingers
    2.
    发明申请
    Position dependent recognition of GNN nucleotide triplets by zinc fingers 有权
    锌指的GNN核苷酸三联体的位置依赖性识别

    公开(公告)号：US20080242847A1

    公开(公告)日：2008-10-02

    申请号：US11893341

    申请日：2007-08-15

    Applicant: Qiang Liu ,  Edward Rebar ,  Andrew C. Jamieson

    Inventor： Qiang Liu ,  Edward Rebar ,  Andrew C. Jamieson

    IPC: C07K14/00

    CPC classification number: C07K14/00 ,  C07K1/00 ,  C07K14/47 ,  C07K14/4702 ,  C07K2299/00 ,  C12N15/102 ,  C12N15/62 ,  C12Q1/6886 ,  C12Q2600/156 ,  G01N33/5308 ,  G01N33/57484 ,  G01N33/6875 ,  G01N2500/04

    Abstract: The specificity of binding of a zinc finger to a triplet or quadruplet nucleotide target subsite depends upon the location of the zinc finger in a multifinger protein and, hence, upon the location of its target subsite within a larger target sequence. The present disclosure provides zinc finger amino acid sequences for recognition of triplet target subsites having the nucleotide G in the 5′-most position of the subsite, that have been optimized with respect to the location of the subsite within the target site. Accordingly, the disclosure provides finger position-specific amino acid sequences for the recognition of GNN target subsites. This allows the construction of multi-finger zinc finger proteins with improved affinity and specificity for their target sequences, as well as enhanced biological activity.

    Abstract translation: 锌指结合到三联体或四核苷酸靶位点的特异性取决于锌指在多角蛋白中的位置，并且因此取决于其目标亚位点在较大靶序列内的位置。 本公开提供了锌指氨基酸序列，用于识别具有子部位的5'-最多位置的核苷酸G的相对于靶位点内的亚位点的位置优化的三重态目标亚位点。 因此，本公开提供了用于识别GNN靶亚属的手指位置特异性氨基酸序列。 这允许构建具有改善其靶序列的亲和性和特异性的多指锌指蛋白，以及增强的生物活性。

    Position dependent recognition of GNN nucleotide triplets by zinc fingers
    6.
    发明授权
    Position dependent recognition of GNN nucleotide triplets by zinc fingers 有权
    锌指的GNN核苷酸三联体的位置依赖性识别

    公开(公告)号：US08383766B2

    公开(公告)日：2013-02-26

    申请号：US11893341

    申请日：2007-08-15

    Applicant: Qiang Liu ,  Edward Rebar ,  Andrew C. Jamieson

    Inventor： Qiang Liu ,  Edward Rebar ,  Andrew C. Jamieson

    IPC: C07K14/00

    CPC classification number: C07K14/00 ,  C07K1/00 ,  C07K14/47 ,  C07K14/4702 ,  C07K2299/00 ,  C12N15/102 ,  C12N15/62 ,  C12Q1/6886 ,  C12Q2600/156 ,  G01N33/5308 ,  G01N33/57484 ,  G01N33/6875 ,  G01N2500/04

    Abstract: The specificity of binding of a zinc finger to a triplet or quadruplet nucleotide target subsite depends upon the location of the zinc finger in a multifinger protein and, hence, upon the location of its target subsite within a larger target sequence. The present disclosure provides zinc finger amino acid sequences for recognition of triplet target subsites having the nucleotide G in the 5′-most position of the subsite, that have been optimized with respect to the location of the subsite within the target site. Accordingly, the disclosure provides finger position-specific amino acid sequences for the recognition of GNN target subsites. This allows the construction of multi-finger zinc finger proteins with improved affinity and specificity for their target sequences, as well as enhanced biological activity.

    Abstract translation: 锌指结合到三联体或四核苷酸靶位点的特异性取决于锌指在多角蛋白中的位置，并且因此取决于其目标亚位点在较大靶序列内的位置。 本公开提供了锌指氨基酸序列，用于识别具有子部位的5'-最多位置的核苷酸G的相对于靶位点内的亚位点的位置优化的三重态目标亚位点。 因此，本公开提供了用于识别GNN靶亚属的手指位置特异性氨基酸序列。 这允许构建具有改善其靶序列的亲和性和特异性的多指锌指蛋白，以及增强的生物活性。

    Regulation of angiogenesis with zinc finger proteins
    7.
    发明授权
    Regulation of angiogenesis with zinc finger proteins 失效
    用锌指蛋白调节血管生成

    公开(公告)号：US08071564B2

    公开(公告)日：2011-12-06

    申请号：US12553826

    申请日：2009-09-03

    Applicant: Edward Rebar ,  Andrew Jamieson ,  Qiang Liu ,  Pei-Qi Liu ,  Alan Wolffe ,  Stephen P. Eisenberg ,  Eric Jarvis

    Inventor： Edward Rebar ,  Andrew Jamieson ,  Qiang Liu ,  Pei-Qi Liu ,  Alan Wolffe ,  Stephen P. Eisenberg ,  Eric Jarvis

    IPC: A61K48/00 ,  C07H21/02

    CPC classification number: A61K48/005 ,  A61K38/00 ,  A61K48/00 ,  C07K14/47 ,  C07K14/4702 ,  C07K14/52 ,  C07K2319/00 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2740/13043 ,  C12N2799/021 ,  C12N2810/85 ,  C12N2830/008

    Abstract: Provided herein are a variety of methods and compositions for regulating angiogenesis, such methods and compositions being useful in a variety of applications where modulation of vascular formation is useful, including, but not limited to, treatments for ischemia and wound healing. Certain of the methods and compositions accomplish this by using various zinc finger proteins that bind to particular target sites in one or more VEGF genes. Nucleic acids encoding the zinc finger proteins are also disclosed. Methods for modulating the expression of one or more VEGF genes with the zinc finger proteins and nucleic acids are also disclosed. Such methods can also be utilized in a variety of therapeutic applications that involve the regulation of endothelial cell growth. Pharmaceutical compositions including the zinc finger proteins or nucleic acids encoding them are also provided.

    Abstract translation: 本文提供了用于调节血管生成的各种方法和组合物，所述方法和组合物可用于血管形成调节是有用的多种应用，包括但不限于缺血和伤口愈合的治疗。 某些方法和组合物通过使用结合一个或多个VEGF基因中的特定靶位点的各种锌指蛋白来实现。 还公开了编码锌指蛋白的核酸。 还公开了用锌指蛋白和核酸调节一种或多种VEGF基因的表达的方法。 这些方法也可用于涉及内皮细胞生长调节的各种治疗应用中。 还提供了包括锌指蛋白或编码它们的核酸的药物组合物。

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